|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 Protein Forms an SCF Ubiquitin Ligase and Regulates Hypocotyl Elongation显示文摘由 ubiquitin proteasome 系统(不间断电源) 的蛋白质周转的规定是在优核质的主要 posttranslational 机制。UPS,的关键部件之一 COP9 signalosome (CSN ) ,调整 cullinring E3 ubiquitin ligases。在植物, CSN 参予多样的细胞、发展的进程,从到房间周期控制的轻发信号。在这个工作,我们孤立新植物特定的交往 CSN F 盒子蛋白质,我们它给 CFK1 (COP9 交往 F 盒子 KELCH 1 ) 取名。我们证明在 Arabidopsis thaliana, CFK1 是功能的 ubiquitin ligase 建筑群的一个部件。我们也证明那 CFK1 稳定性被 CSN 并且由 proteasome 依赖的解朊作用调整,并且那盏灯在胚轴导致 CFK1 抄本的累积。CFK1 的分析击倒,异种,和 overexpressing 幼苗显示 CFK1 由增加支持胚轴延伸房间尺寸。CSN 层次的减小提高弄空 CFK1 的幼苗的短胚轴显型,当 CSN 活动的完全的损失压制 CFK1-overexpressing 幼苗的长胚轴的显型时。我们建议那 CFK1 (并且它由 CSN 的规定) 细胞的机制的一个新奇部件正在控制胚轴延伸。 | Anna Franciosini Benedetta Lombardi Silvia lafrate Valeria.Pecce Giovanni Mele Leonardo Lupacchini Gianmarco Rinaldi Youichi Kondou Giuliana Gusmaroli Shiori Aki Tomohiko Tsuge Xing-Wang Deng Minami Matsui Paola Vittorioso Paolo Costantin Giovanna Serino | 2013 | Molecular Plant2013，6，5： | 3 |
| 2 | MsJ1, an alfalfa DnaJ-like gene, is tissue-specific and transcriptionally regulated during cell cycle显示文摘 | Giovanna Frugis Giovanni Mele Donato Giannino Domenico Mariotti | 1999 | Plant Molecular Biology1999，，3： | 1 |
| 3 | Transcriptome driven characterization of curly-and smooth-leafed endives reveals molecular differences in the sesquiterpenoid pathway显示文摘Endives(Cichorium endivia L.)are popular vegetables,diversified into curly/frisée-and smooth/broad-leafed(escaroles)cultivar types(cultigroups),and consumed as fresh and bagged salads.They are rich in sesquiterpene lactones(STL)that exert proven function on bitter taste and human health.The assembly of a reference transcriptome of 77,022 unigenes and RNA-sequencing experiments were carried out to characterize the differences between endives and escaroles at the gene structural and expression levels.A set of 3177 SNPs distinguished smooth from curly cultivars,and an SNP-supported phylogenetic tree separated the cultigroups into two distinct clades,consistently with the botanical varieties of origin(crispum and latifolium,respectively).A pool of 699 genes maintained differential expression pattern(core-DEGs)in pairwise comparisons between curly vs smooth cultivars grown in the same environment.Accurate annotation allowed the identification of 26 genes in the sesquiterpenoid biosynthesis pathway,which included several germacrene A synthase,germacrene A oxidase and costunolide synthase members(GAS/GAO/COS module),required for the synthesis of costunolide,a key precursor of lactucopicrin-and lactucin-like sesquiterpene lactones.The core-DEGs contained a GAS gene(contig83192)that was positively correlated with STL levels and recurrently more expressed in curly than smooth endives,suggesting a cultigroup-specific behavior.The significant positive correlation of GAS/GAO/COS transcription and STL abundance(2.4-fold higher in frisée endives)suggested that sesquiterpenoid pathway control occurs at the transcriptional level.Based on correlation analyses,five transcription factors(MYB,MYB-related and WRKY)were inferred to act on contig83192/GAS and specific STL,suggesting the occurrence of two distinct routes in STL biosynthesis. | Giulio Testone Giovanni Mele Elisabetta di Giacomo Gian Carlo Tenore Maria Gonnella Chiara Nicolodi Giovanna Frugis Maria Adelaide Iannelli Giuseppe Arnesi Alessandro Schiappa Tiziano Biancari Donato Giannino | 2019 | Horticulture Research2019，6，1： | 1 |
| 4 | Leaf nutrient content and transcriptomic analyses of endive (Cichorium endivia) stressed by downpour-induced waterlog reveal a gene network regulating kestose and inulin contents显示文摘Endive(Cichorium endivia L.),a vegetable consumed as fresh or packaged salads,is mostly cultivated outdoors and known to be sensitive to waterlogging in terms of yield and quality.Phenotypic,metabolic and transcriptomic analyses were used to study variations in curly-(‘Domari’,‘Myrna’)and smooth-leafed(‘Flester’,‘Confiance’)cultivars grown in short-term waterlog due to rainfall excess before harvest.After recording loss of head weights in all cultivars(6-35%),which was minimal in‘Flester’,NMR untargeted profiling revealed variations as influenced by genotype,environment and interactions,and included drop of total carbohydrates(6–50%)and polyols(3–37%),gain of organic acids(2–30%)and phenylpropanoids(98–560%),and cultivar-specific fluctuations of amino acids(−37 to+15%).The analysis of differentially expressed genes showed GO term enrichment consistent with waterlog stress and included the carbohydrate metabolic process.The loss of sucrose,kestose and inulin recurred in all cultivars and the sucrose-inulin route was investigated by covering over 50 genes of sucrose branch and key inulin synthesis(fructosyltransferases)and catabolism(fructan exohydrolases)genes.The lowered expression of a sucrose gene subset together with that of SUCROSE:SUCROSE-1-FRUCTOSYLTRANSFERASE(1-SST)may have accounted for sucrose and kestose contents drop in the leaves of waterlogged plants.Two anti-correlated modules harbouring candidate hub-genes,including 1-SST,were identified by weighted gene correlation network analysis,and proposed to control positively and negatively kestose levels.In silico analysis further pointed at transcription factors of GATA,DOF,WRKY types as putative regulators of 1-SST. | Giulio Testone Anatoly Petrovich Sobolev Giovanni Mele Chiara Nicolodi Maria Gonnella Giuseppe Arnesi Tiziano Biancari Donato Giannino | 2021 | Horticulture Research2021，8，1： | 0 |
| 5 | 基于中空单元的夹层平板结构设计方法研究(英文)显示文摘目的:大型的桁架结构或空间刚架结构可以认为是用中空单元组合而成的宏观结构,在结构初步设计时,如何设计排布中空基本单元以满足结构的性能要求是设计的关键。本文旨在探讨夹层平板结构,在给定竖向变形控制指标后,如何使用简化的方法快速设计各层中空基本单元的尺寸和空隙程度。创新点:1.对于水立方这种复杂的空间多面体网格结构,本文将其理想化为连续的中空介质,利用微观力学中的均质化分析方法,将结构分解为多个基本单元(RVE)进行分析,从而推导出结构性能指标与单元参数之间的关系。2.对于大跨度平板结构,本文提出一种基于RVE重复叠加组合的设计方法,并利用有限元法对该方法进行检验。方法:1.对夹层平板结构的弯曲特性进行理论分析,根据Kirchhoff-Love理论,得到板弯曲刚度与各层的截面惯性矩之间的关系;2.通过理论推导出结构弯曲刚度(图3)、材料使用量(图4)随层厚度和层密度的变化关系;3.从基本单元出发,利用均质化分析方法分别对中空立方体(图5)和空间桁架结构(图6)进行分析,得到结构抗弯刚度随单元中空程度和距截面中性轴距离的函数关系(图7和8),进而推导出结构参数化设计的拟合公式;4.利用该设计公式,针对一个90 m×90 m×2.5 m的平板结构,以跨中挠度为跨度的1/500为设计指标,分别设计由两种基本单元组成的夹层平板结构,并将结构挠度的有限元计算结果与设计指标进行比较(图12)。结论:1.本文提出的利用中空单元组成的夹层平板结构的设计方法,通过优化设计中空单元的各项参数,可以在保证性能指标的同时最大化降低结构质量,得到最优刚度质量比。2.本设计方法和有限元法得到的结果之间差异非常小,说明该设计方法准确可靠。 | Gianpaolo PERRELLA Giovanni Maria MONTUORI Massimiliano FRALDI Elena MELE | 2016 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2016，17，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员