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| 1 | Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression显示文摘AIM: To establish stable tetracycline-inducible pancreatic cancer cell lines. METHODS: Suit-2, MiaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-M2), under the control of either a cytomegalovirus (CMV) or a chicken β-actin promoter, and the resulting clones were characterised.RESULTS: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2E1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and Ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable. | Sarah Tonack Sabina Patel Mehdi Jalali Taoufik Nedjadi Rosalind E Jenkins Christopher Goldring John Neoptolemos Eithne Costello | 2011 | World Journal of Gastroenterology2011，17，15： | 4 |
| 2 | First report of dollar spot caused by sclerotinia honoeocarpa on Agrostis stolonifera in Arentina显示文摘 | Rivera M C Wright E R Goldring L V | 2004 | Plant Disease2004，88，12： | 1 |
| 3 | Extract of Ginkgo biloba induces phase 2 genes through Keapl-Nrf2-ARE signaling pathway显示文摘 | LIU X P Goldring C E Copple I M | 2007 | Life Sci2007，80，17： | 1 |
| 4 | Ichnofabrics containing Ophiomorpha:significance in shallow-water facies interpretation Journal of the Geological Society显示文摘 | Pollard J E Goldring R Buck S G | 1993 | London1993，150，： | 1 |
| 5 | The Nrf2 cell defence pathway: Keapl-dependent and -independent mechanisms of regulation 显示文摘 | BRYAN H K OLAYANJU A GOLDRING C E | 2013 | Bio- chemical Pharmacology2013，85，6： | 1 |
| 6 | Experience with lesions that mimic gliomas in patients presenting with a chronic seizure disorder显示文摘 | Goldring S Gregorie E M | | 0，，： | 1 |
| 7 | The Nrf2 cell defence pathway: Keapl - dependent and - independent mechanisms of regulation 显示文摘 | BRYAN H K OLAYANJU A GOLDRING C E | 2013 | Bioehem Pharmacol2013，85，6： | 1 |
| 8 | Pathogenesis of bone erosions in rheumatoid arthritis 显示文摘 | GOLDRING S R GRAVALLESE E M | 2000 | Curr Opin Rheumatol2000，12，3： | 1 |
| 9 | Increased constitutive c-Jun N-terminal kinase signaling in mice lacking glutathione S-transfcrase Pi 显示文摘 | Elsby R Kitteringham N R Goldring C E Lovatt C A | 2003 | J Biol Chem2003，278，22： | 1 |
| 10 | Developing a psychometrically sound assessment of school leadership:The VAL-ED as a case study显示文摘 | Porter A C Polikoff M S Goldring E | 2010 | Edu- cational Administration Quarterly2010，46，2： | 1 |
| 11 | First report of dollar spot caused by Sclereotinia homoecarpa on Agrostis stolon(fera in Arentina显示文摘 | Rivera M C Wright E R Goldring L V | 2004 | Plant Disease2004，，5： | 1 |
| 12 | Cellular mechanisms and the roles of cytokines in bone erosions in rheumatoid显示文摘 | RaVallese E M Goldring S R | 2000 | Arthritis Rheum2000，43，2： | 1 |
| 13 | Pathologenesis of bone erosions in rheumatoid arthritis 显示文摘 | Goldring S R Gravallese E M | 2000 | Curr 0pin Rheumatoid2000，12，4： | 1 |
| 14 | Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis 显示文摘 | Gravallese E M Goldring S R | 2000 | Arthritis Rheum2000，43，10： | 1 |
| 15 | The hepatotoxic metabolite of acetaminophen directly activates the Keapl-Nrf2 cell defense system 显示文摘 | Copple I M Goldring C E Jenkins R E | 2008 | Hepatolgy2008，48，4： | 1 |
| 16 | Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis 显示文摘 | Gravallese E M Goldring S R | 2000 | Arthritis Rheum2000，43，10： | 1 |
| 17 | Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis显示文摘 | Goldring S R | 2000 | Arthritis Rheum2000，43，10： | 1 |
| 18 | Sustaining Teacher Commitment: The Role of Professional Communities显示文摘 | Hausman C S Goldring E B | 2001 | PJE: Peabody Journal of Education2001，76，2： | 1 |
| 19 | Aeterogeneity in hormone responses and patterns of collagen synthesis in cloned dermal fibroblasts显示文摘 | Goldring SR Stepenson ML Dwnie E | 1990 | Jcl in Invest1990，85，3： | 1 |
| 20 | Extract of Ginkgo bi- loba induces the phase 2 genes through Keapl-Nrf2-ARE signaling pathway显示文摘 | liu X P Goldring C E Copple I M | 2007 | Life Sci2007，80，17： | 1 |
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