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| 1 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 2 | Two modifications in the treatment of keratocystic odontogenic tumors (KCOT) and the use of Caruoy's solution (CS)-a retrospective study lasting between 2 and 10 years 显示文摘 | Gosau M Draenert FG Muller S | 2010 | Clin Oral Investig2010，14，1： | 1 |
| 3 | Maxillary Sinus Anatomy:A Cadaveric Study with Clinical Implications 显示文摘 | Gosau M Rink DD Driemel O | 2009 | The Anatomical Redord2009，292，： | 1 |
| 4 | Salivary gland carci-nomas显示文摘 | Ettl T Schwarz-Furlan S Gosau M | 2012 | Oral Maxillofac Surg2012，16，3： | 1 |
| 5 | Ewing sarcoma of the mandible mimicking an odontogenic abscess- a case report显示文摘 | Gosau M Baumhoer D Ihrler S | 2008 | Head Face Med2008，4，： | 1 |
| 6 | Salivary gland carcino- mas显示文摘 | Ettl T Schwarz-Furlan S Gosau M | 2012 | Oral Maxillofac Surg2012，16，3： | 1 |
| 7 | Genome-wide gene expression profiles of dental follicle stem cells 显示文摘 | Vollkommer T Gosau M Felthaus O | 2015 | Acta Odontol Scand2015，73，2： | 1 |
| 8 | Genome-wide gene expression profiles of dental follicle stem cells 显示文摘 | Vollkommer T Gosau M Fehhaus O | 2015 | Acta Odontol Stand2015，73，2： | 1 |
| 9 | Distinct signaling pathways mediate cardiomyocyte phospholipase D stimulation by endothelin-1 and thrombin显示文摘 | Fahimi-Vahid M Gosau N Michalek C | 2002 | J Mol Cell Cardiol2002，34，： | 1 |
| 10 | Maxillary sinus anato- my : a cadaveric study with clinical implications 显示文摘 | Gosau M Rink D Driemel O | 2009 | Anat Rec (Hoboken)2009，292，3： | 1 |
| 11 | Lipopolysaccharide from Escherichia coli but not from Porphyromonas gingivalis induce pro-inflammatory cytokines and alkaline phosphatase in dental follicle cells显示文摘 | Christian O. Morsczeck Johannes Dress Martin Gosau | 2012 | Archives of Oral Biology2012，，12： | 1 |
| 12 | Two modifi cations in the treatment of keratocystie odontogenic tumors (KCOT) and the use of Carnoy's solution (CS) a retrospective study lasting between 2 and 10 years显示文摘 | Gosau M Draenert FG Muller S | 2010 | Clin Oral Investig2010，14，： | 1 |
| 13 | Long-term outcome and subjective quality of life after surgical treatment of lower lip cancer显示文摘 | Schüller M Gosau M Müller S | 2015 | Clin Oral Investig2015，19，5： | 1 |
| 14 | Maxillary sinus anatomy: a cadaveric study with clinical implications 显示文摘 | Gosau M Rink D Driemel O | 2009 | Anat Rec (Hoboken)2009，292，3： | 1 |
| 15 | Cancer stem celllike cells from a single cell of oral squamous carcinoma cell lines显示文摘 | Felthaus O Ettl T Gosau M | 2011 | Biochem Biophys Res Commun2011，407，1： | 1 |
| 16 | Management of venous thrombosis in fibular free osseomusculocutaneous flaps used for mandibular reconstruction:clinical techniques and treatment considerations 显示文摘 | Draenert FG Gosau M A1 NB | 2010 | Head Face Med2010，6，： | 1 |
| 17 | Cancer stem cell - like cells from a single cell of oral squamous carcinoma cell lines 显示文摘 | FELTHAUS O ETYL T GOSAU M | 2011 | Biochem Biophys Res Commun2011，407，1： | 1 |
| 18 | Effect of six different peri-implantitis disinfection methods on in vivo human oral biofilm显示文摘 | Gosau M Hahnel S Schwarz F | 2010 | Clin Oral Implants Res2010，21，8： | 1 |
| 19 | Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines显示文摘 | Felthaus O Ettl T Gosau M | 2011 | Biochem Biophys Res Commun2011，407，1： | 1 |
| 20 | Collagen I induces the expression of alkaline phosphatase and osteopontin via Independent activations of FAK and ERK signalling pathways显示文摘 | Viale-Bouroncle S Gosau M Morsczeck C | 2014 | Arch Oral Biol2014，59，12： | 1 |
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