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| 1 | Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals显示文摘尽管 myocyte 房间移植研究为心肌的梗塞建议了一个有希望的治疗学的潜力,到为心肌的修理的临床的治疗的发展的一个主要障碍是与为移植获得相对同类的室的 myocytes 联系的困难。人的胚胎的干细胞(hESCs ) 是 cardiomyocytes 的有希望的来源。这里,我们报导发信号的 retinoid 在 hESCs 的心脏的区别期间调整 atrial 对室的 myocytes 的命运说明。我们发现少量和 pan-retinoic 酸受体对手 BMS-189453 (RAi ) 显著地增加了 hESCs 的心脏的区别效率。为了调查 retinoid,工作,我们把对待 Noggin+RAi 的文化与对待 Noggin+RA 的文化作比较。我们的结果证明室特定的基因 IRX-4 的表示层次极端地在对待 Noggin+RAi 的文化被提高。MLC-2V,另一个室特定的标记,在对待 Noggin+RAi 的文化,然而并非在对待 Noggin+RA 的文化的 cardiomyocytes 在 cardiomyocytes 的多数被表示。流动 cytometry 分析和 electrophysiological 研究与 64.7 | Qiangzhe Zhang Junjie Jiang Pengcheng Han Qi Yuan Jing Zhang Xiaoqian Zhang Yanyan Xu Henghua Cao Qingzhang Meng Li Chen Tian Tian Xin Wang Pu Li Jurgen Hescheler Guangju Ji Yue Ma | 2011 | Cell Research2011，21，4： | 10 |
| 2 | GCN2 deficiency protects mice from denervation-induced skeletal muscle atrophy via inhibiting FoxO3a nuclear translocation显示文摘 | Yuting Guo Huiwen Wang Yinglong Tang Yue Wang Mengqi Zhang Zhiguang Yang Eric Nyirimigabo Bin Wei Zhongbing Lu Guangju Ji | 2018 | Protein & Cell2018，9，11： | 3 |
| 3 | RyR2 proteins contribute to the formation of Ca^2+ sparks in smooth muscle显示文摘 | Guangju JI Feldman ME Kotlikoff MI | 2004 | J Gen Physiol2004，123，4： | 1 |
| 4 | Generation of Calhm1 knockout mouse and characterization of calhm1 gene expression显示文摘Alzheimer’s disease(AD)is the most common neurode-generative disease among elderly people worldwide.Several genes have been validated to be associated with AD,and calcium homeostasis modulator 1(Calhm1)is the latest suspected one.To investigate the biological and pathological function of Calhm1 systematically,we generated a Calhm1 conventional knockout mouse.However,both the male and female of elderly Calhm1 knockout(KO)mice showed similar ability to their wild type littermates in spatial learning and memory retrieving.Surprisingly,we found that Calhm1 mRNA could not be detected in mouse brains at different ages,although it is expressed in the human brain tissues.We further found that CpG islands(CGIs)of both mouse and human Calhm1 were hypermethylated,whereas CGI of mouse Calhm2 was hypomethylated.In addition,transcriptional active marker H3K4Di occupied on promoters of human Calhm1 and mouse Calhm2 at a considerable level in brain tissues,while the occupancy of H3K4Di on pro-moter of mouse Calhm1 was rare.In sum,we found that mouse Calhm1 was of rare abundance in brain tissues.So it might not be suitable to utilize the knockout murine model to explore biological function of Calhm1 in the pathogenesis of AD. | Junbing Wu Shengyi Peng Rong Wu Yumin Hao Guangju Ji Zengqiang Yuan | 2012 | Protein & Cell2012，3，6： | 1 |
| 5 | ERp44 C160S/C212S mutants regulate IP_(3)R_(1) channel activity显示文摘Previous studies have indicated that ERp44 inhibits inositol 1,4,5-trisphosphate(IP3)-induced Ca2+release(IICR)via IP3R1,but the mechanism remains largely unexplored.Using extracellular ATP to induce intracellular calcium transient as an IICR model,Ca2+image,pull down assay,and Western blotting experiments were carried out in the present study.We found that extracellular ATP induced calcium transient via IP3Rs(IICR)and the IICR were markedly decreased in ERp44 overexpressed Hela cells.The inhibitory effect of C160S/C212S but not C29S/T396A/ΔT(331–377)mutants of ERp44 on IICR were significantly decreased compared with ERp44.However,the binding capacity of ERp44 to L3V domain of IP3R1(1L3V)was enhanced by ERp44 C160S/C212S mutation.Taken together,these results suggest that the mutants of ERp44,C160/C212,can more tightly bind to IP3R1 but exhibit a weak inhibition of IP3R1 channel activity in Hela cells. | Congyan Pan Ji Zheng Yanyun Wu Yingxiao Chen Likun Wang Zhansong Zhou Wenxuan Yin Guangju Ji | 2011 | Protein & Cell2011，2，12： | 0 |
| 6 | Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement显示文摘Genetically encoded Ca^(2+) indicators(GECI)are important for the measurement of Ca^(2+) in vivo.GCaMP2,a widelyused GECI,has recently been iteratively improved.Among the improved variants,GCaMP3 exhibits significantly better fluorescent intensity.In this study,we developed a new GECI called GCaMPJ and determined the crystal structures of GCaMP3 and GCaMPJ.GCaMPJ has a 1.5-fold increase in fl uorescence and 1.3-fold increase in calcium affi nity over GCaMP3.Upon Ca^(2+) binding,GCaMP3 exhibits both monomeric and dimeric forms.The structural superposition of these two forms reveals the role of Arg-376 in improving monomer performance.However,GCaMPJ seldom forms dimers under conditions similar to GCaMP3.St ructural and mutagenesis studies on Tyr-380 confi rmed its importance in blocking the cpEGFPβ-barrel holes.Our study proposes an efficient tool for mapping Ca^(2+) signals in intact organs to facilitate the further improvement of GCaMP sensors. | Yingxiao Chen Xianqiang Song Sheng Ye Lin Miao Yun Zhu Rong-Guang Zhang Guangju Ji | 2013 | Protein & Cell2013，4，4： | 0 |
| 7 | Dissociation of FK506 Binding Protein 12.6 from Ryanodine Receptor Type 2 Is Regulated by cADPR but not β-Adrenergic Stimulation in Mouse Cardiomyocytes显示文摘AIMS: β-adrenergic augmentation of Ca2+ sparks and cardiac contractility has been functionally linked to phosphorylation-dependent dissociation of FK506 binding protein 12. | Xu Zhang, Zheng Chen, Bin Wei,Lin Miao,Guangju Ji Institute of Biophysics Chinese Academy of Sciences, 15 Datun Rd.Chaoyang District, Beijing 100101, China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 8 | Insulin/PI3K/AKt Signaling Pathway Mediates Cardiac Hypertrophy in FKBP12.6 Deficient Male Mice显示文摘Male mice deficient in FKBP12.6 display cardiac hypertrophy, but the underlying mechanism is still unclear. Here we report that the cardiac hypertrophy is a physiological response | Zheng Chen, Zheng-Zheng Li, Wen-Xuan Yin, Guangju Ji Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 | 2009 | 生物物理学报2009，25，S1： | 0 |
| 9 | Ca^(2+) Release Induced by cADP-Ribes Is Mediated by FKBP12.6 Proteins in Mouse Bladder Smooth Muscle显示文摘Ca2+ release through ryanodine receptors (RyRs) plays an important role in excitation-contraction coupling in heart and smooth muscle. FKBP12.6 proteins | Ji Zheng1,Xu Zhang2, Jinhong Pan1,Bin Wei2, Bo Song1 and Guangju Ji2 1 Urological Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.2 National Laboratory of Biomacromolecules,Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd, Beijing 100101, China | 2009 | 生物物理学报2009，25，S1： | 0 |
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