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| 1 | 不同-10区保守序列的tac启动子表达睾丸酮丛毛单胞菌中的活化因子显示文摘提取睾丸酮丛毛单胞菌(Comamonastestosteroni)的基因组DNA,利用PCR扩增活化因子(activator)基因。将扩增产物克隆到pKtac1(含强启动子tac)中,构建含全长activator基因(564bp)的重组质粒pKtac1-act1和含部分activator基因(409bp)的pKtac1-act2。测序结果发现重组质粒pKtac1-act2的tac启动子-10保守区由TATAAT突变为TATGTT。将pKtac1-act1和pKtac1-act2分别进行酶切和连接,获得重组质粒pKtac1-act3(含启动子-10保守区为TATAAT,409bp的部分activator基因)和pKtac1-act4(含启动子-10保守区为TATGTT,564bp的全长activator基因)。将4个重组质粒分别转化入宿主菌Es-cherichiacoliHB101中,用酶联免疫分析方法测定细菌总蛋白质中的activator表达量;将pAX1(含3α-hsd/CR基因)分别和4个重组质粒一起转化入宿主菌E.coliHB101中,测定细菌总蛋白质中的3α-hsd/CR和activator的表达量。结果表明:启动子-10保守区为TATAAT的质粒比-10保守区为TATGTT的质粒有更高的转录活性;过量的actvitor表达可能影响质粒pK-tac1-act3表达的稳定性;启动子-10区保守序列的下降突变(pKtac1-act2,TATAAT→TATGTT)及tac启动子的丢失(pK-tac1-act3,继代培养4次)揭示了宿主菌E.coliHB101的自身防护机制。 | 戴艺民 周以飞 Guangming Xiong 吴明霞 潘大仁 | 2005 | 农业生物技术学报2005，13，6： | 8 |
| 2 | Optical Micro/Nano Fibers Enabled Smart Textiles for Human-Machine Interface显示文摘Wearable human-machine interface(HMI)is an advanced technology that has a wide range of applications from robotics to augmented/virtual reality(AR/VR).In this study,an optically driven wearable human-interactive smart textile is proposed by integrating a polydimethylsiloxane(PDMS)patch embedded with optical micro/nanofibers(MNF)array with a piece of textiles.Enabled by the highly sensitive pressure dependent bending loss of MNF,the smart textile shows high sensitivity(65.5 kPa^(−1))and fast response(25 ms)for touch sensing.Benefiting from the warp and weft structure of the textile,the optical smart textile can feel slight finger slip along the MNF.Furthermore,machine learning is utilized to classify the touch manners,achieving a recognition accuracy as high as 98.1%.As a proof-of-concept,a remote-control robotic hand and a smart interactive doll are demonstrated based on the optical smart textile.This optical smart textile represents an ideal HMI for AR/VR and robotics applications. | Shuqi Ma Xiaoyu Wang Pan Li Ni Yao Jianliang Xiao Haitao Liu Zhang Zhang Longteng Yu Guangming Tao Xiong Li Limin Tong Lei Zhang | 2022 | Advanced Fiber Materials2022，4，5： | 5 |
| 3 | Hydroxycalciopyrochlore, A New Mineral Species from Sichuan, China显示文摘Hydroxycalciopyrochlore, ideally(Ca,Na,U,□)2(Nb,Ti)2O6(OH), cubic, is a new mineral species(IMA2011-026) within the pyrochlore supergroup that was found occurring at the Maoniuping mine, Mianning County, Xichang prefecture, Sichuan Province, southwest China. The mineral is found in an alkali feldspar granite rare-earth ore deposit(26–27 Ma). Associated minerals include calcite, barite, celestine, albite, aegirine, aegirine-augite, fluorite, parasite-(Ce), thorite, thorianite, zircon, galena, sphalerite, magnetite, and pyrite. Crystals occur mostly as octahedra, and less often as dodecahedra and tetrahexahedra or combinations thereof. Some occur with an allotriomorphic habit with a thick triangular tabular form. Crystals generally range from 0.1 to 1 mm in size. The mineral is brownishblack, greenish-black and black on fresh sections with a brown streak. The crystal is translucent, and has a greasy lustre on fresh sections. It is metamict without any observed parting or cleavage and with a conchoidal fracture. The Vickers microhardness is 572 kg/mm2(5–6 on the Mohs hardness scale). The density measured by hydrostatic weighing is 5.10(3) g/cm3. The strongest four reflections in the X-ray powder-diffraction pattern [d in(I) hkl] are: 2.9657(100) 2 2 2, 1.8142(34) 0 4 4, 1.5463(21) 2 2 6, 2.5688(18) 0 0 4. The unit-cell parameters are a = 10.381(4), V = 1118.7(7)3, Z = 8. The structure was solved and refined in the space group Fd3m with R = 0.09. The empirical formula is(Ca0.74Na0.58U0.40Ce0.05Fe0.02□0.21)2.00(Nb1.15Ti0.80Ta0.03Al0.01Mg0.01)2.00O6.02 [(OH)1.01F0.09]1.10, on the basis of 2 atoms of B pfu; the simplified formula is(Ca,Na,U,□)2(Nb,Ti)2O6(OH). Type material is deposited in the Geological Museum of China, Beijing, People's Republic of China, catalogue number M11800. | YANG Guangming LI Guowu XIONG Ming PAN Baoming YAN Chenjie | 2014 | Acta Geologica Sinica(English Edition)2014，88，3： | 3 |
| 4 | Regulation of 3α-hydroxysteroid dehydrogenase/carbonyl reductase in Comamonas testosteroni: function and relationship of two operators 显示文摘 | Guangming Xiong Stefarmie Markowetz Edmund Maser | 2003 | Chemico-Biological Interactions2003，，： | 1 |
| 5 | DETERMINATION OF CHLORPYRIFOS AND ITS MAIN DEGRADATION PRODUCT TCP IN WATER SAMPLES BY DISPERSIVE LIQUID–LIQUID MICROEXTRACTION BASED ON SOLIDIFICATION OF FLOATING ORGANIC DROPLET COMBINED WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY显示文摘 | Jianfei Xiong Guangming Zhou Zhuo Guan Xiangyu Tang Qiang He Limin Wu | 2014 | Journal of Liquid Chromatography & Related Technologies2014，，11： | 1 |
| 6 | Identification of a new steroid degrading bacterial strain H5 from the Baltic Sea and isolation of two estradiol inducible genes显示文摘 | Yingying Sang Guangming Xiong Edmund Maser | 2011 | Journal of Steroid Biochemistry and Molecular Biology . 2011 (1-2)2011，，1： | 1 |
| 7 | Characterization of the steroid degrading bacterium S19-1 from the Baltic Sea at Kiel, Germany显示文摘 | Tingdi Zhang Guangming Xiong Edmund Maser | 2010 | Chemico-Biological Interactions2010，，1： | 1 |
| 8 | Steroid degradation and two steroid-inducible enzymes in the marine bacterium H5显示文摘 | Yingying Sang Guangming Xiong Edmund Maser | 2011 | Chemico-Biological Interactions2011，，1： | 1 |
| 9 | Characterization of the steroid degrading bacterium S19-1 from the Baltic Sea at Kiel, Germany显示文摘 | Tingdi Zhang Guangming Xiong Edmund Maser | 2010 | Chemico-Biological Interactions2010，，1： | 1 |
| 10 | Combining AHP with GIS in Synthetic Evaluation of Eco-environment Quality-A Case Study of Hunan Province, China显示文摘 | Xiong Ying Zeng Guangming Chen Guiqiu | 2007 | Ecologi cal Modeling2007，209，： | 1 |
| 11 | Combining AHP with GIS in synthetic evaluation of eco-environment quality-A case study of Hunan Province显示文摘 | Ying Xiong Zeng Guangming Chen Guiqiu Tang Lin Wang Kelin Huang Daoyou | 2007 | Ecological Modelling2007，209，24： | 1 |
| 12 | Combining AHP with GIS in synthetic evaluation of eco- environment quality: A case study of Hunan Province, China显示文摘 | Xiong Ying Zeng Guangming Chen Guiqiu | 2007 | Ecological Modeling2007，209，： | 1 |
| 13 | Thefuzzy-AHP evaluation method for unmanned groundvehicles显示文摘 | SUN Yang TAO Gang XIONG Guangming | 2013 | Applied Mathematics & InformationSciences2013，7，2： | 1 |
| 14 | De- termination of chlorpyrifos and its main degradation product TCP in water samples by dispersive liquid-liquid microextrae- tion based on solidification of floating organic droplet com- bined with high-performance liquid chromatography显示文摘 | XIONG Jianfei ZHOU Guangming GUAN Zhuo | 2014 | Jour- nal of Liquid Chromatography Related Technologies2014，37，11： | 1 |
| 15 | Male germ cell apoptosis and epigenetic hlstone modification induced by tripterygium wilfordii Hook F显示文摘 | Ji Xiong Hu Wang Guangming Guo | | 0，，06： | 1 |
| 16 | Identification ofa new steroid degrading bacterial strain H5 fromthe Baltic Sea and isolation of two estradiol induc-ible genesV 显示文摘 | Yingying Sang Guangming Xiong | 2011 | Elsevier2011，，： | 1 |
| 17 | Combining AHP with GIS in synthetic evaluation of eco-environment quality: A case study of Hunan Province, China显示文摘 | YING Xiong ZENG Guangming CHEN Guiqiu | 2007 | Ecological Modeling2007，209，： | 1 |
| 18 | The Effect of Laser Irradiation on Surface Enhanced Raman Scatering for Silver Film显示文摘TheEfectofLaserIrradiationonSurfaceEnhancedRamanScateringforSilverFilmShengRongsheng,ZhouGuangming,XiongJian,XuZhisan,Michae... | Sheng Rongsheng Zhou Guangming Xiong Jian Xu Zhisan Michael D. Morris Zeng Yun'e | 1997 | Wuhan University Journal of Natural Sciences1997，2，1： | 1 |
| 19 | A model on the regulation of 3α - hydroxysteroid dehydrogenase: carbonyl reductase expression in Comamonas testosteroni 显示文摘 | Guangming Xiong Hans-Jorg M Andreas B | 2001 | Chemico-Biological Interactions2001，，： | 1 |
| 20 | Dynamic Path Following Control of a Ground Ackerman Steering Robot to Avoid a Collision显示文摘A novel method is proposed to dynamically control the path following of a ground Ackerman steering robot to avoid a collision.The method consists of collision prediction module,collision avoidance module and global path following module.The elliptic repulsive potential field method(ER-PFM)and the enhanced vector polar histogram method(VPH+)based on the Ackerman steering model are proposed to predict the collision in a dynamic environment.The collision avoidance is realized by the proposed cost function and speed control law.The global path following process is achieved by pure pursuit.Experiments show that the robot can fulfill the dynamic path following task safely and efficiently using the proposed method. | Guangming Xiong Xiaoyun Li | 2017 | Journal of Beijing Institute of Technology2017，26，2： | 0 |
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