|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function显示文摘 | Guohao Dai a Olga Tsukurov Roslyn Orkia | 2000 | Jouinal of Vascular Surgery2000，32，5： | 1 |
| 2 | Generation of dynamically perfused functional vascular network system within hydrogel using3D bio-printing technology显示文摘Introduction:3D bio-printing technology[1-3](Figure 1)capable of dispensing live cells,soluble factors,and phase-changing hydrogel in a desired pattern,has great potential in creating 3D tissue.However,maintaining the viability of a thick tissue structure during tissue growth and maturation is challenging due to lack of adequate vascular perfusion.In a simple tissue model with | Vivian K.Lee Seung-Schik Yoo Peter Vincent Guohao Dai | 2013 | 医用生物力学2013，28，S1： | 1 |
| 3 | The integration of 3-D cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds显示文摘 | Lingling Zhao Vivian K Lee Seung-Schik Yoo Guohao Dai Xavier Intes | | 0，，07： | 1 |
| 4 | Entropy-driven phase regulation of high-entropy transition metal oxide and its enhanced high-temperature microwave absorption by in-situ dual phases显示文摘High-temperature microwave absorbers are significant for military equipment which experiences severe aerodynamic heat.In this work,high-entropy oxide(HEO)(FexCoNiCrMn)mOn with excellent high-temperature microwave absorption is studied.Driven by the effect of entropy,the composition of the oxide can be transformed from spinel-type phase(FexCoNiCrMn)_(3)O_(4) to corundum-type phase(FexCoNiCrMn)2O3 with the increasing content of iron.Only spinel-type or corundum-type structure composes the oxide when x≤3 or x≥5.But in-situ dual phases can coexist when x equals 4 during phase transition.Interestingly,obliged to abundant heterogeneous interfaces and crystal defects in the dual-phase HEO,magnetic property,dielectric polarization,and microwave loss ability are all well enhanced.The Smith chart analysis demonstrates the impedance matching condition is well improved due to the enhanced loss ability.These findings pave a new way for the adjustment of electromagnetic properties of HEO by entropy-driven phase regulation.Meanwhile,the dual-phase absorber can achieve better than 90%absorption in 9.6-12.4 GHz at 800℃ with a thickness of 2.6 mm,a low thermal diffusivity of 0.0038 cm^(2)/s at 900℃,and excellent high-temperature stability,which indicates it’s promising as a high-temperature microwave absorber. | Guohao Dai Ruixiang Deng Xiao You Tao Zhang Yun Yu Lixin Song | 2022 | Journal of Materials Science & Technology2022，，21： | 1 |
| 5 | An in vitro cell culture system to study the influence of external pneumatic compression on endothelial function显示文摘 | Guohao Dai MS a Olga Tsukurov Roslyn Orkia | | 0，，05： | 1 |
| 6 | 3D direct printing of mechanical and biocompatible hydrogel meta-structures显示文摘Direct Ink Writing(DIW)has demonstrated great potential as a versatile method to 3D print multifunctional structures.In this work,we report the implementation of hydrogel meta-structures using DIW at room temperature,which seamlessly integrate large specific surface areas,interconnected porous characteristics,mechanical toughness,biocompatibility,and water absorption and retention capabilities.Robust but hydrophobic polymers and weakly crosslinked nature-origin hydrogels form a balance in the self-supporting ink,allowing us to directly print complex meta-structures without sacrificial materials and heating extrusion.Mechanically,the mixed bending or stretching of symmetrical re-entrant cellular lattices and the unique curvature patterns are combined to provide little lateral expansion and large compressive energy absorbance when external forces are applied on the printed meta-structures.In addition,we have successfully demonstrated ear,aortic valve conduits and hierarchical architectures.We anticipate that the reported 3D meta-structured hydrogel would offer a new strategy to develop functional biomaterials for tissue engineering applications in the future. | Lei Zhang Wenhan Lee Xinhao Li Yanhui Jiang Nicholas Xuanlai Fang Guohao Dai Yongmin Liu | 2022 | Bioactive Materials2022，7，4： | 0 |
| 7 | A neuron-immune circuit regulates neurodegeneration in the hindbrain and spinal cord of Arf1-ablated mice显示文摘Neuroimmune connections have been revealed to play a central role in neurodegenerative diseases(NDs).However,the mechanisms that link the central nervous system(CNS)and peripheral immune cells are still mostly unknown.We recently found that specific ablation of the Arf1 gene in hindbrain and spinal cord neurons promoted NDs through activating the NLRP3 inflammasome in microglia via peroxided lipids and adenosine triphosphate(ATP)releasing.Here,we demonstrate that IL-1βwith elevated chemokines in the neuronal Arf1-ablated mouse hindbrain and spinal cord recruited and activatedγδT cells in meninges.The activatedγδT cells then secreted IFN-γthat entered into parenchyma to activate the microglia-A1astrocyte-C3-neuronal C3a R neurotoxic pathway.Remarkably,the neurodegenerative phenotypes of the neuronal Arf1-ablated mice were strongly ameliorated by IFN-γor C3 knockout.Finally,we show that the Arf1-reduction-induced neuroimmune-IFN-γ-gliosis pathway exists in human NDs,particularly in amyotrophic lateral sclerosis and multiple sclerosis.Together,our results uncover a previously unknown mechanism that links the CNS and peripheral immune cells to promote neurodegeneration. | Guohao Wang Shuhan Jin Jiaqi Liu Xu Li Peng Dai Yuetong Wang Steven X.Hou | 2023 | National Science Review2023，10，12： | 0 |
| 8 | Evaluation of photochemistry reaction kinetics to pattern bioactive proteins on hydrogels for biological applications显示文摘Bioactive signals play many important roles on cell function and behavior.In most biological studies,soluble biochemical cues such as growth factors or cytokines are added directly into the media to maintain and/or manipulate cell activities in vitro.However,these methods cannot accurately mimic certain in vivo biological signaling motifs,which are often immobilized to extracellular matrix and also display spatial gradients that are critical for tissue morphology.Besides biochemical cues,biophysical properties such as substrate stiffness can influence cell behavior but is not easy to manipulate under conventional cell culturing practices.Recent development in photocrosslinkable hydrogels provides new tools that allow precise control of spatial biochemical and biophysical cues for biological applications,but doing so requires a comprehensive study on various hydrogel photochemistry kinetics to allow thorough photocrosslink reaction while maintain protein bioactivities at the same time.In this paper,we studied several photochemistry reactions and evaluate key photochemical parameters,such as photoinitiators and ultra-violet(UV)exposure times,to understand their unique contributions to undesired protein damage and cell death.Our data illustrates the retention of protein function and minimize of cell health during photoreactions requires careful selection of photoinitiator type and concentration,and UV exposure times.We also developed a robust method based on thiol-norbornene chemistry for independent control of hydrogel stiffness and spatial bioactive patterns.Overall,we highlight a class of bioactive hydrogels to stiffness control and site specific immobilized bioactive proteins/peptides for the study of cellular behavior such as cellular attraction,repulsion and stem cell fate. | Taylor B.Dorsey Alexander Grath Annling Wang Cancan Xu Yi Hong Guohao Dai | 2018 | Bioactive Materials2018，3，1： | 0 |
| 9 | Biomechanical forces in atherosclerosis-susceptible and -resistant regions of human vasculature differentially regulate endothelial vaso-protective phenotypes显示文摘Introduction Atherosclerosis is a potentially life-threatening disease of large arteries that is strongly associated with systemic risk factors such as hypercholesterolemia,hypertension,smoking,and diabetes. However,atherosclerosis develops as a | Guohao Dai(Department of Biomedical Engineering,Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy,NY 12180,USA) | 2010 | 医用生物力学2010，25，S1： | 0 |
| 10 | A platinum@polymer-catechol nanobraker enables radio-immunotherapy for crippling melanoma tumorigenesis, angiogenesis, and radioresistance显示文摘Malignant melanoma cell-intrinsic PD-1:PD-L1 interaction thrusts tumorigenesis,angiogenesis,and radioresistance via mTOR hyperactivation to aggravate circumjacent aggression.Interdicting melanoma intrinsic growth signals,including the blockade of PD-L1 and mTOR signaling concurrently,cooperative with radiotherapy may provide a vigorous repertoire to alleviate the tumor encumbrance.Thence,we design a three-pronged platinum@polymer-catechol nanobraker to deliver mTOR inhibitor TAK228 and anti-PD-L1 antibody(aPD-L1)for impeding the melanoma-PD-1-driven aggression and maximizing the melanoma eradication.The aPD-L1 collaborated with TAK228 restrains melanoma cell-intrinsic PD-1:PD-L1 tumorigenic interaction via blocking melanoma-PD-L1 ligand and the melanoma-PD-1 receptor-driven mTOR signaling;corresponding downregulation of mTOR downstream protumorigenic cellular MYC and proangiogenic hypoxia-inducible factor 1-alpha is conducive to preventing tumorigenesis and angiogenesis,respectively.Further,high-Z metal platinum sensitizing TAK228-enhanced radiotherapy confers the nanobraker on remarkable tumoricidal efficacy.Hereto,the customized three-pronged nanobrakers efficiently suppress melanoma tumorigenesis and angiogenesis concomitant with the amplification of radiotherapeutic efficacy.Such an ingenious tactic may provide substantial benefits to clinical melanoma patients. | Wenxi Li Jie Yan Hao Tian Bei Li Guohao Wang Wei Sang Zhan Zhang Xuanjun Zhang Yunlu Dai | 2023 | Bioactive Materials2023，，4： | 0 |
| 11 | NIR-Ⅱ-absorbing conjugated polymer-based theranostic agent for NIR-Ⅱfluorescence imaging-guided photothermal therapy acting synergistically with tumor microenvironment-responsive nitric oxide therapy显示文摘Despite tremendous advances in gas therapy,there are major concerns about the inevitable concentration of toxicity and the ability to perform real-time tracking of drug delivery.Second near-infrared(NIR-Ⅱ)window absorbing nanoplatforms hold great promise for precision medicine because of their excellent tissue penetration of light and non-invasive nature.In this study,we engineered an NIR-Ⅱlaser-activated theranostic agent(named CP-bF@PEG)that was composed of amphiphilic polymers(Pluronic F127,with polyethylene glycol,PEG,moieties)coated with an NIR-Ⅱ-absorbing conjugated polymer(PTTBBT,CP)and nitric oxide(NO)donor(benzofuroxan,bF),which served as an NIR-Ⅱphotothermal inducer and NO nanogenerator.Under deep tissue penetration of NIR-Ⅱlaser irradiation,CP-bF@PEG was found to possess fluorescence imaging ability to accurately identify tumor and excellent photothermal effect.Moreover,CP-bF@PEG could generate NO via glutathione activation in the tumor microenvironment in a controllable manner.This NIR-Ⅱ-absorbing polymer for high-contrast NIR-Ⅱfluorescence imaging-guided precision photothermal therapy achieved synergistic effects with NO therapy,as evidenced by pronounced tumor therapeutic efficacy and few side effects.This nanotheranostic agent is a highly promising candidate for high-contrast NIR-Ⅱimaging-guided precision photothermal therapy combined with gas therapy against cancer. | Jie Li Lisi Xie Wei Sang Wenxi Li Guohao Wang Hao Tian Zhan Zhang Jianhua Liu Quli Fan Yunlu Dai | 2022 | ChemPhysMater2022，1，1： | 0 |
| 12 | A'three musketeers'tactic for inclining interferon-γ as a comrade-in-arm to reinforce the synergistic-tumoricidal therapy显示文摘Interferon-γ(IFN-γ),secreted by activated T cells predominantly,plays a crucial performance in the tumoricidal immune response.Unfortunately,a high level of IFN-γseverely ignites the immunosuppressive response,especially by increasing the expression of immune checkpoint programmed death-ligand 1(PD-L1)and immunoregulatory enzyme indoleamine 2,3-dioxygenase 1(IDO-1).Herein,we have explored a versatile IFN-γ-nano-integrator(aPD-L1-SH@Ce6@NLG919-PEG,simplified as CNDP)to establish a positive anti-tumor feedback loop to amplify the IFN-γ-mediated tumoricidal effect.In this nanointegrator,photosensitizer chlorin e6(Ce6)mediates photodynamic therapy(PDT)to re-shape immunogenicity and activate the adaptive immune response,followed by the secretion of high-level IFN-γto struggle tumor cells.IDO-1 inhibitor(NLG919)afterwards mitigates the immunosuppressive behavior of IFN-γby neutralizing the function of IDO-1.To turn“waste”into wealth,anti-PD-L1(aPD-L1)antibodies are technically integrated into the nano-integrator to propel the precise attack of breast cancer through ascending PD-L1 blockade.Together,this“three musketeers”nano-integrator tumoricidal tactic may give a unique insight into the clinical anti-tumor therapy. | Wenxi Li Lisi Xie Yi Ju Zhan Zhang Bei Li Jie Li Wei Sang Guohao Wang Hao Tian Yunlu Dai | 2022 | Nano Research2022，15，4： | 0 |
| 13 | IR780/Gemcitabine-conjugated metal-phenolic network enhanced photodynamic cancer therapy显示文摘Photodynamic therapy (PDT) is a clinically approved cancer treatment that uses energy of light to generate active substances that cause damage to the cancer. Photosensitizers are employed to absorb light and generate toxic reactive oxygen species (ROS) to damage biomolecules like DNA. At the same time, some chemotherapy drugs like nucleotide analogues can provide mechanism-guided promotion in the treatment efficacy of PDT. However, the photosensitizer and chemotherapy drugs used in PDT is usually organic molecules, which suffers from bad solubility, fast clearance, and acute toxicity. To achieve targeted treatment, a reasonable delivery system is necessary. Therefore, we reported a metal-phenolic network where IR780 and gemcitabine were coupled chemically to overcome these shortcomings. The enhanced PDT effects can be realized by the promoted cell death both in vitro and in vivo. Moreover, the synergistic therapy also induced T-cell mediated anti-tumor immune response, which was significant for the inhibition of distant tumor growth. This work expanded the biomedical application of metal-phenolic materials and contribute to the wider application of photodynamic cancer therapy. | Songtao Zhou Hao Tian Jie Yan Zhan Zhang Guohao Wang Xinying Yu Wei Sang Bei Li Greta S.P.Mok Jie Song Yunlu Dai | 2024 | Chinese Chemical Letters2024，35，1： | 0 |
| 14 | 3D Bio-Printed Glioblastoma-Vascular Niche Models显示文摘Introduction Glioblastoma multiforme(GBM),a malignant brain tumor,is highly invasive and use brain microvessels to migrate and invade.Studying the perivascular invasion/migration of GBM may enable new possibilities in GBM therapy.However,the lack proper 3D study models that recapitulate GBM hallmarks restricts investigating cell-cell/cell-molecular interactions in tumor microenvironments.In this study,we created GBM-vascular niche models through 3D bioprinting [1-2] using patient-derived GBM cells with sternness(GSC:glioblastoma stem cells),vasculature endothelial cells(ECs),mural cells,and various hydrogels.Materials and methods Three GBM-vascular models were designed:Model A with large vessels and GBM spheroid;Model B with large-and micro-vessels,and GBM spheroid;Model C with large-and micro-vessels and scattered GBM cells.Large channels were created by sacrificial bioprinting.Microvessel network was formed through self-assembly of ECs(HUVEC or brain EC)and mural cells(fibroblast,pericytes,and/or astrocytes).Three GBM cell types were used in the study:SD02 and SD03 are GSCs;U87MG is a commercially-available GBM cell line.Collagen type I or fibrin hydrogel have been used as major scaffold materials.For drug treatment,Temozolomide in culture medium was perfused through large vasculatures in Model A.Results and discussion Three different GBM-vascular models were successfully fabricated and culture for 2-10.GSCs cultured in these models maintained sternness and heterogeneity during the long-term cultures.In Model A,GSCs actively invaded into the surrounding tissues(~Day26),initially regressed in response to the drug(~Day50),then developed therapeutic resistance and resumed aggressive invasion(~Day57).In Model B and C,three GBM types presented distinctive invasion patterns and EC-interactions.SD02 cells showed a spiky invasion pattern with elongated morphology.SD03 cells showed a more dispersed invasion pattern with many single cell migrations towards surrounding microvessels.U87MG cells showed a blunt invasion pattern,caused EC death in the spheroid form;however,the EC death was significantly reduced in the scattered single cell form.Conclusions In this study,we have created GBM-vascular niche models that can recapitulate various GBM characteristics such as cancer sternness,tumor type-specific invasion patterns,and drug responses with therapeutic resistance.Our models have a great potential in investigating patient-specific tumor behaviors under chemo-/radio-therapy conditions and consequentially helping to tailor personalized treatment strategy.The model platform is capable of modifying multiples variables including ECMs,cell types,vascular structures,and dynamic culture condition.Thus,it can be adapted to other biological systems and serve as a valuable tool for generating customized microenvironments. | Vivian KLee Hongyan Zou Roland Friedel Guohao Dai | 2019 | 医用生物力学2019，34，A01： | 0 |
| 15 | Advances in extracellular vesicle-based combination therapies for spinal cord injury显示文摘Spinal cord injury is a severe insult to the central nervous system that causes persisting neurological deficits.The currently available treatments involve surgical,medical,and rehabilitative strategies.However,none of these techniques can markedly reverse neurological deficits.Recently,extracellular vesicles from various cell sources have been applied to different models of spinal cord injury,thereby generating new cell-free therapies for the treatment of spinal cord injury.However,the use of extracellular vesicles alone is still associated with some notable shortcomings,such as their uncertainty in targeting damaged spinal cord tissues and inability to provide structural support to damaged axons.Therefore,this paper reviews the latest combined strategies for the use of extracellular vesicle-based technology for spinal cord injury,including the combination of extracellular vesicles with nanoparticles,exogenous drugs and/or biological scaffold materials,which facilitate the targeting ability of extracellular vesicles and the combinatorial effects with extracellular vesicles.We also highlight issues relating to the clinical transformation of these extracellular vesicle-based combination strategies for the treatment of spinal cord injury. | Tingting Wang Guohao Huang Zhiheng Yi Sihan Dai Weiduan Zhuang Shaowei Guo | 2024 | Neural Regeneration Research2024，19，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员