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您的检索式：作者名="Guozheng SHEN"
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| 1 | A study of miRNAs targets prediction and experimental validation显示文摘microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA target genes will help a lot by understanding their biological functions.Sophisticated computational approaches for miRNA target prediction,and effective biological techniques for validating these targets now play a central role in elucidating their functions.Owing to the imperfect complementarity of animal miRNAs with their targets,it is difficult to judge the accuracy of the prediction.Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets.To date,only a few miRNAs targets are confirmed.In this article,we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals. | Yong Huang Quan Zou Haitai Song Fei Song Ligang Wang Guozheng Zhang Xingjia Shen | 2010 | Protein & Cell2010，1，11： | 7 |
| 2 | An intelligent controller for the vehicle standing starts based on the rules and knowledge 显示文摘 | Shen Shuiwen Yan Guozheng Zhang Jianwu etc | | SAE0，，： | 2 |
| 3 | Quantitative detection of Cymbidium mosaic virus by real time PCR显示文摘The technique of SYBR Green-based quantitative real-time reverse transcription polymerase chain reaction(real-time RT-PCR)was applied to quantitative detect a 764 bp nucleotide sequence containing total coat protein(cp)gene of Cymbidium mosaic virus(CyMV).The plasmid containing the target sequence was constructed to prepare the standard curve and detect the sensitivity.The standard curve was drawn based on the linear relationship between the logarithm(base 10)of the quantity of target sequence and cycle threshold[C(T)].While the concentration of plasmid DNA falling within the range of 2.6×10^(7)to 2.6×10^(2)copies per tube established a regression equation,y=-0.3583x+10.32,and related coefficient:r^(2)=0.995.The real-time RT-PCR assay for CyMV had a minimum detectable quantity of two copies per tube.The naturally infected samples of Phalaenopsis sp.and the artificially inoculated samples of Arachnis sp.with trace CyMV were quantitatively detected using this method.CyMVin the positive samples of Phalaenopsis sp.and Arachnis sp.was confirmed by DNA sequencing and cp gene homeology blast.The results showed that CyMV extracted from the leaves of orchid in Hangzhou,Zhejiang Province,China,could be derived from Kunming city(KM),Yunnan Province,China.This method characterized by high sensitivity,specificity,and precision is suitable for early diagnosis and quantitative detection of CyMV. | Aichun LIU Yun ZHAO Songlin RUAN Guozheng SHEN | 2009 | Frontiers in Biology2009，4，3： | 1 |
| 4 | Status of water environment pollution in the Xixi Wetland and its ecological treatment countermeasures显示文摘In this paper,the water quality of the Xixi Wetland was evaluated and the characteristics of water pollution were described according to the survey data.Based on the status of water quality and its functional requirements as an urban wetland,biological-ecological countermeasures were suggested.The experimental use of ecological technologies,such as artificial wetlands,ecological aquiculture and artificial floating island,were done in several fish ponds in the Xixi Wetland.Water monitoring results show that the quality of the treated water has improved significantly and the measures to purify the eutrophic water in the wetland have been effective. | Wenyue CHEN Jiemin ZHENG Zhongquan LI Guozheng SHEN Yuchen LI | 2009 | Frontiers in Biology2009，4，2： | 0 |
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