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	题名	作者	年代	出处	被引量
1	Circulating tumor cells in pancreatic cancer patients:Enrichment and cultivation显示文摘AIM:To investigate the feasibility of separation and cultivation of circulating tumor cells(CTCs)in pancreatic cancer(Pa C)using a filtration device.METHODS:In total,24 Pa C patients who were candidates for surgical treatment were enrolled into the study.Peripheral blood samples were collected before an indicated surgery.For each patient,approximately8 m L of venous blood was drawn from the antecubitalveins.A new size-based separation Meta Cell?technology was used for enrichment and cultivation of CTCs in vitro.(Separated CTCs were cultured on a membrane in FBS enriched RPMI media and observed by inverted microscope.The cultured cells were analyzed by means of histochemistry and immunohistochemistry using the specific antibodies to identify the cell origin.RESULTS:CTCs were detected in 16 patients(66.7%)of the 24 evaluable patients.The CTC positivity did not reflect the disease stage,tumor size,or lymph node involvement.The same percentage of CTC positivity was observed in the metastatic and non-metastatic patients(66.7%vs 66.7%).We report a successful isolation of CTCs in Pa C patients capturing proliferating cells.The cells were captured by a capillary action driven size-based filtration approach that enabled cells cultures from the viable CTCs to be unaffected by any antibodies or lysing solutions.The captured cancer cells displayed plasticity which enabled some cells to invade the separating membrane.Further,the cancer cells in the'bottom fraction',may represent a more invasive CTC-fraction.The CTCs were cultured in vitro for further downstream applications.CONCLUSION:The presented size-based filtration method enables culture of CTCs in vitro for possible downstream applications.	Vladimir Bobek Robert Gurlich Petra Eliasova Katarina Kolostova	2014	World Journal of Gastroenterology2014，20，45：	15
2	Physiology and genetics of procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
3	Serum leptin levels in septic men correlate well with C-reactive protein (CRP) and TNF-alpha but not with BMI显示文摘	Maruna P Gurlich R Frasko R	2001	Physiol Res2001，50，6：	1
4	Physiology and genetics of larocalcitonin显示文摘	IMaruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
5	Physiology and genetics of pro calcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
6	Physiology and genet- icsof procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，，：	1
7	Physio- logy and genetics of procalcitonin 显示文摘	Manma P Nedelnkova K Gurlich R	2000	Physiol Res2000，49，：	1
8	Physiology and genetics of proealcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
9	Physiology and genetics of procal- citonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
10	Reliability of composite in a ran- dom stress state 显示文摘	M ark R Gurlich R Byron Pipes	1998	Composites Science and Technology1998，58，6：	1
11	Physiology and genetics of procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiological Research2000，49，：	1
12	Physiology and genetics of procalcitongin显示文摘	Mcuruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，，：	1
13	Randomized clinical trial of ito- pride for the treatment of postoperative ileus after laparoscopic chole- cystomy 显示文摘	Gurlich R Frasko R Manana P	2004	Chir Gastroentero12004，20，61：	1
14	Physiology and genetics of procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
15	Physiology andgenetics of procalcitonin 显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，，：	1
16	Physiology and genetics of Procalcition显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physioa Res2000，49，11：	1
17	Physiology and genetics of procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R	2000	Physiol Res2000，49，1：	1
18	Prediction and monitoring of severe acute pancreatitis显示文摘	Gurlich R Maruna P Spicdk J	2006	Cas Lek Cesk2006，145，6：	1
19	physiology nd genentics of proculciton 显示文摘	Maruna p Nedelnikova K Gurlich 12	2000	physiol Res2000，49，：	1
20	Physiology and genetics of procalcitonin显示文摘	Maruna P Nedelnikova K Gurlich R		0，，01：	1

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