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| 1 | Genome Editing with CRISPR-Cas9:Can It Get Any Better?显示文摘The CRISPR-Cas revolution is taking place in virtually all fields of life sciences.Harnessing DNA cleavage with the CRISPR-Cas9 system of Streptococcus pyogenes has proven to be extraordinarily simple and efficient,relying only on the design of a synthetic single guide RNA(sgRNA) and its co-expression with Cas9.Here,we review the progress in the design of sgRNA from the original dual RNA guide for S.pyogenes and Staphylococcus aureus Cas9(SpCas9 and SaCas9).New assays for genome-wide identification of offtargets have provided important insights into the issue of cleavage specificity in vivo.At the same time,the on-target activity of thousands of guides has been determined.These data have led to numerous online tools that facilitate the selection of guide RNAs in target sequences.It appears that for most basic research applications,cleavage activity can be maximized and off-targets minimized by carefully choosing guide RNAs based on computational predictions.Moreover,recent studies of Cas proteins have further improved the flexibility and precision of the CRISPR-Cas toolkit for genome editing.Inspired by the crystal structure of the complex of sgRNA-SpCas9 bound to target DNA,several variants of SpCas9 have recently been engineered,either with novel protospacer adjacent motifs(PAMs) or with drastically reduced off-targets.Novel Cas9 and Cas9-like proteins called Cpf 1 have also been characterized from other bacteria and will benefit from die insights obtained from SpCas9.Genome editing with CRISPR-Cas9 may also progress with better understanding and control of cellular DNA repair pathways activated after Cas9-induced DNA cleavage. | Maximilian Haeussler Jean-Paul Concordet | 2016 | Journal of Genetics and Genomics2016，43，5： | 5 |
| 2 | Amine-catalyzed polycyclotrimerization of arylene bipropiolate:A metal-free and regioselective route to hyperbranched polymer显示文摘This paper presents a new synthetic route to hyperbranched polymer. An arylene bipropiolate monomer,dimethylmethylenebis(1,4-phenylene) bipropiolate,was prepared by a one-step esterification reaction of bisphenol A with propiolic acid. Polycyclotrimrtization of the monomer was effected by reflux in dimethylformamide(DMF),producing a soluble hyperbranched poly(aroxycarbonylphenylene) in a good yield. The polymer was perfectly branched with a degree of branching of 100%. | JIM Cathy K W LAM Jacky W Y HAEUSSLER Matthias | 2008 | Science China Chemistry2008，51，8： | 3 |
| 3 | Progression of mycosis fungoides is associated with increasing cutaneous expression of interleukin-10 Mrna显示文摘 | Asadullah K Docke WD Haeussler A | 1996 | J Invest Dermatol1996，107，6： | 1 |
| 4 | Strategic alliancesand product development in high technology newfirms : the moderating effect of technological capabilities显示文摘 | HAEUSSLER C PA TZELT H ZA H R A S A | 2012 | Journal of Business V enturing2012，27，2： | 1 |
| 5 | Malignant Fibrous Histiocytoma of Bone:Conventional X-ray and MR Imaging Features显示文摘 | Link TM Haeussler MD Poppek S | 1998 | Skeletal Radiol1998，27，10： | 1 |
| 6 | Information-sharing in academia and the industry:A comparative study显示文摘 | Haeussler C | 2011 | Research Policy2011，40，1： | 1 |
| 7 | Malignant fibrous histiocytoma of bone:conventional X-ray and MR imaging features 显示文摘 | Link TM Haeussler MD Poppek S | 1998 | SkeletalRadiol1998，271，： | 1 |
| 8 | How patenting informs VC investors – The case of biotechnology显示文摘 | Carolin Haeussler Dietmar Harhoff Elisabeth Mueller | 2014 | Research Policy2014，，： | 1 |
| 9 | Free reacrive oxygen species and nephrotoxicity of contrast agents显示文摘 | Haeussler U Riedei M Keller F | 2004 | Kidney Blood Press Res2004，27，3： | 1 |
| 10 | Regulation of cell physiology and pathology by protein S-glutathionylation: lessons learned from the cardiovascular system 显示文摘 | Pimentel D Haeussler D J Matsui R | 2012 | Antioxid Redox Sig- nal2012，16，6： | 1 |
| 11 | Free reactive oxygen species and nephrotoxicity of contrust agents显示文摘 | HAEUSSLER U RIEDEL M KELLER F | 2004 | Kidney Blood Pressure Research2004，27，3： | 1 |
| 12 | When needles look like hay:how to find tissue-specific enhancers in model organism genomes显示文摘 | Haeussler M Joly JS | | 0，，02： | 1 |
| 13 | Geochemical studies of the Tongyong gold-silver deposits, republic of Korea: Evi- dence of meteoric water dominace in a Te-bearing epithermal system显示文摘 | Shelton K L So C S Haeussler G T | 1990 | Economic Geology1990，85，6： | 1 |
| 14 | How patenting in- forms VC investors: The case of biotechnology显示文摘 | Haeussler C Harhoff D Miller E | 2014 | Re- search Policy2014，43，8： | 1 |
| 15 | Link between ridge subduction and gold mineralization in Southern Alaska显示文摘 | Haeussler P J Bradley D C Goldfarb R J | 1995 | Geology1995，23，： | 1 |
| 16 | Cultivation of malting wheat显示文摘 | Haeussler T | 1985 | Brauwelt1985，125，6： | 1 |
| 17 | Facial talon cusps显示文摘 | McNarnara T Haeussler AM Keane J | 1997 | Lnt J Paediatr Dent1997，7，4： | 1 |
| 18 | Breaking the ivory tower: Ac- ademic entrepreneurship in the life sciences in UK and Germany显示文摘 | Haeussler C Colyvas J A | 2011 | Research Policy2011，40，1： | 1 |
| 19 | Free reactive oxygen species and nephrotoxicity of contrast agents显示文摘 | Haeussler U Riedel M Keller F | 2004 | Kidney & Blood Pressure Research2004，27，3： | 1 |
| 20 | Free reactive oxygen spe-cies and nephrotoxicity of contrast gents显示文摘 | Haeussler U Riedel M Keller F | 2004 | Kidney Blood PressBes2004，27，3： | 1 |
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