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| 1 | Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China显示文摘The objective of the present investigation was to estimate the prevalence of Toxoplasma gondii infection and co-infection with porcine reproductive and respiratory syndrome virus(PRRSV), classical swine fever virus(CSFV) and porcine circovirus type 2(PCV-2) in pigs in China. A total of 372 tissues or serum samples collected from pigs distributed in 9 provinces/municipalities of China during the period from February 2011 to November 2012 were assayed for T. gondii antigens and antibodies using enzyme linked immunosorbent assay(ELISA) technique, while the PCR was designed for the detection of the PRRSV, CSFV and PCV-2, respectively. The total positive rate of T. gondii, PRSSV, CSFV and PCV-2 was 9.14%(34/372), 50.00%(186/372), 37.10%(138/372) and 3.23%(12/372), respectively. Among the 34 T. gondii positive samples, 26 samples were simultaneously infected with T. gondii and viruses, while the remaining eight samples were infected with T. gondii alone. In addition, the co-infection rate of T. gondii with PRSSV, T. gondii with PRSSV and CSFV, T. gondii with PRSSV and PCV-2, T. gondii with CSFV and PCV-2, T. gondii with PRSSV, CSFV and PCV-2 was 1.61%(6/372), 4.03%(15/372), 0.27%(1/372), 0.27%(1/372) and 0.81%(3/372), respectively. The results of the present survey revealed that PRRSV and CSFV were the common pathogens co-existing with porcine toxoplasmosis in China, and both of them could increase the chances of T. gondii infection in pig. This is the first report of T. gondii co-infections with viruses in pigs. It is very important to understand the interactions of parasite and virus, and can be used as reference data for the control and prevention of co-infections of T. gondii and viruses in pigs. | WANG Shuai ZHANG Meng LIU Xin-chao LIN Tao YANG Han-chun YUAN Shi-shan ZHAO Guang-wei IA Hassan YAN Ruo-feng SONG Xiao-kai XU Li-xin LI Xiang-rui | 2015 | Journal of Integrative Agriculture2015，14，9： | 2 |
| 2 | Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human,cat and swine in chicken显示文摘In this study,four strains of Toxoplasma gondii with the same genetic type(Type I) originated from chicken,human,cat and swine were used to compare the immune responses in resistant chicken host to investigate the relationships between the parasite origins and the pathogenicity in certain host.A total of 300,10-day-old chickens were allocated randomly into five groups which named JS(from chicken),CAT(from cat),CN(from swine),RH(from human) and a negative control group(—Ve) with 60 birds in each group.Tachyzoites of four different T.gondii strains(JS,CAT,CN and RH) were inoculated intraperitoneally with the dose of 1×10~7 in the four designed groups,respectively.The negative control(-Ve) group was mockly inoculated with phosphate-buffered saline(PBS) alone.Blood and spleen samples were obtained on the day of inoculation(day 0) and at days 4,11,25,39 and 53 post-infection to screen the immunopathological changes.The results demonstrated some different immune characters of T.gondii infected chickens with that of mice or swine previous reported.These differences included up-regulation of major histocompatibility complex class Ⅱ(MHC Ⅱ) molecules in the early stage of infection,early peak expressions of interleukin(IL)-12(IL-12) and-10(IL-10) and long keep of IL-17.These might partially contribute to the resistance of chicken to T.gondii infection.Comparisons to chickens infected with strains from human,cat and swine,chickens infected with strain from chicken showed significant high levels of CD4^+ and CD8^+ T cells,interferon gamma(IFN-γ),IL-12 and IL-10.It suggested that the strain from chicken had different ability to stimulate cellular immunity in chicken. | ZHAO Guang-wei WANG Shuai WANG Wang ZHANG Zhen-chao XIE Qing ZHANG Meng IA Hassan YAN Ruo-feng SONG Xiao-kai XU Li-xin LI Xiang-rui | 2015 | Journal of Integrative Agriculture2015，14，5： | 1 |
| 3 | Hypoglycemic and antihyperglycemic effects of Trigonella foenum - graecum leaf in normal and alloxan induced diabetic rats显示文摘 | Abdel - Barry JA Abdel - Hassan IA Al - Hakiem MH | 1997 | Ethnopharmacol1997，149，： | 1 |
| 4 | Interaction of copper with iron,iodine,and thyroid hormone status in goitrous patient显示文摘 | Tasneem GK Ghulam AK Hassan IA | | 0，，： | 1 |
| 5 | Antibodies to mutated citrullinated vimentin in rheumatoid arthritis: diagnostic value, associ- ation with radiologieal damage and axial skeleton affection 显示文摘 | Mansour HE Metwaly KM Hassan IA | 2010 | Clin Med Insights Arthritis Musculoskelet Disord2010，3，： | 1 |
| 6 | The light still shines,but not that brightly? The current status of perinatal near infrared spectroscopy显示文摘 | Nicklin SE Hassan IA Wickramasinghe YA | 2003 | Arch Dis Child (Fetal Neonatal Ed)2003，88，4： | 1 |
| 7 | Immunological changes induced by Toxoplasma gondii Glutathione-S-Transferase (TgGST) delivered as a DNA vaceine显示文摘 | Wang S Hassan IA Liu XC | 2015 | Res Veter Sci2015，，99： | 1 |
| 8 | Antibodies to mutated itrullinated vimentin in rheumatoid arthritis: diagnostic value, as- sociation with radiological damage and axial skeleton affection 显示文摘 | Mansour HE Metwaly KM Hassan IA | 2010 | Clin Med Insights Arthritis Musculoskelet Disord2010，3，： | 1 |
| 9 | Audit of laboratory mycology services for the management of patients with fungal infections in the northwest of England显示文摘 | Hassan IA Critten P Isalska B | 2006 | J Clin Pathol2006，59，： | 1 |
| 10 | The light still shines, but not that brightly? The current status of perinatal near infrared spectroscopy 显示文摘 | Nicklin SE Hassan IA Wickramasinghe YA | 2003 | Arch Dis Child (Fetal Neonatal Ed)2003，88，4： | 1 |
| 11 | Evaluation of a training cur- riculum for prehospital trauma ultrasound 显示文摘 | Press GM Miller SK Hassan IA | 2013 | J Emerg Med2013，45，6： | 1 |
| 12 | The light still shines, but not that brightly? The current status of perinatal near infrared spectroscopy 显示文摘 | NICKLIN SE HASSAN IA WICKRAMASINGHE YA | 2003 | Arch Dis Child(Fetal Neonatal Ed)2003，88，4： | 1 |
| 13 | Family history of cancer and associated risk of developing neuroendocrine tumors: a case-control study显示文摘 | Hassan MM Phan A IA D | 2008 | Cancer Epide- miol Biomarkers Prev2008，17，4： | 1 |
| 14 | Thelight still shines,but not that brightly?The current status ofperinatal near infrared spectroscopy显示文摘 | Nicklin SE Hassan IA Wickramasinghe YA | 2003 | Arch Dis Child FetalNeonatal Ed2003，88，4： | 1 |
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