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| 1 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 2 | Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?显示文摘AIM: To evaluate the potential of Polysol, a newly developed preservation solution, in cold storage of small bowel grafts, compared with the current standards, University of Wisconsin solution (UW), Celsior and histidine-tryptophan-ketoglutarate solution (HTK). METHODS: Male Wistar rats were used as donors. Small bowels were retrieved, flushed and then stored in the respective 4 solutions for 18 h at 4℃. Functional integrity of the grafts was evaluated by isolated reperfusion with oxygenated Krebs-Henseleit buffer at 37℃ for 30 min in all 4 groups. RESULTS: Polysol preservation exhibited the highest tissue ATP concentration and the lowest release of LDH. Malondialdehyde, an index for tissue lipid peroxidation, was also the lowest in Polysol. Tissue oxygen consumption was significantly higher in Polysol than in the others. Of interest, UW-storage promoted 10-fold higher apoptosis than in the others. Moreover, electron microscopy revealed that the mucosal villi/micro-villi formation and the cell organelles, including mitochondria, were both significantly better preserved in Polysol, while deleterious alterations were apparent in the others, most notably in UW. Although Celsior and HTK exhibited the better trend of results than UW in some parameters, but could not reach the over-all superiority to UW.CONCLUSION: Cold storage using Polysol resulted in significantly better integrity and function of small bowel grafts than UW. Hence, Polysol may be a novel alternative for the small bowel preservation. | Lai Wei Koichiro Hata Benedict Marie Doorschodt Reinhard Büttner Thomas Minor René H Tolba | 2007 | World Journal of Gastroenterology2007，13，27： | 9 |
| 3 | Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats显示文摘AIM:To investigate oxidative stress(OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage.METHODS: Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant(60% hepatectomy); orthotopic liver transplantation(OLT) with whole liver graft(100% OLT); and split OLT(SOLT) with 40% graft(40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OSinduced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase(PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase(MMP)-9, MMP-2, tissue inhibitor of metalloproteinase(TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. RESULTS: Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. CONCLUSION: Under conditions with an insufficient liver remnant, prevention of OS-induced damage via the Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery. | Tomohide Hori Shinji Uemoto Feng Chen Lindsay B Gardner Ann-Marie T Baine Toshiyuki Hata Takayuki Kogure Justin H Nguyen | 2014 | World Journal of Hepatology2014，6，2： | 7 |
| 4 | Simple and reproducible hepatectomy in the mouse using the clip technique显示文摘AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data. | Tomohide Hori Norifumi Ohashi Feng Chen Ann-Marie T Baine Lindsay B Gardner Toshiyuki Hata Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，22： | 4 |
| 5 | Express of a soybean nodule-enhanced PEP Carboxylase gene that striking similanty to another gene for a house-keeping isoform显示文摘 | Hata S Katsura I Kouchi H | 1998 | Plant J1998，13，2： | 1 |
| 6 | How sensitive are epidermal growth factor receptor-tyrosine kinase inhibitors for squamous cell carcinoma of the lung harboring EGFR gene-sensitive mutations?显示文摘 | Hata A Katakami N Yoshioka H | 2014 | J Thorac Oncol2014，9，： | 1 |
| 7 | New dyeing method for wild silk yarns using reactive dyes显示文摘 | KATO H HATA T OBO M | 1996 | JARQ1996，30，3： | 1 |
| 8 | Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines显示文摘 | Kamata Y Watanabe J Hata H | 2004 | Eur J Gynaecol Oncol2004，25，1： | 1 |
| 9 | Expression of Bcl-2 familly of proteins in fresh myeloma cells显示文摘 | HARDA N HATA H YOSHIDA M | 1998 | Leukemia1998，12，11： | 1 |
| 10 | The characteristics of cultured mucosal cell sheet as a material for grafting;comparison with cultured epidermal cell sheet显示文摘 | Hata K Kagami H Ueda M Torii S Matsuyama M | 1995 | Ann Plast Surg1995，34，5： | 1 |
| 11 | IL-18 together with anti-CD3 antibody induces humanThl cells to pro-duce Thl-and Th2-cytokines and IL-8 显示文摘 | Hata H Yoshimoto T Hayashi N | 2004 | Int Immunol2004，16，12： | 1 |
| 12 | Identification of residual breast carcinoma following neoadjuvant chemotherapy: diffusion- weighted imaginge-comparison with contrast-enhanced MR imaging and pathologic findings 显示文摘 | Woodhams R Kakita S Hata H | 2010 | Radiology2010，254，2： | 1 |
| 13 | Midkine enhances earlystages of collagen gel contraction显示文摘 | Sumi Y Muramatsu H Hata K | 2000 | J Biochem(Tokyo)2000，127，2： | 1 |
| 14 | Urinary excretion of aquaporin-2 in term and preterm infants显示文摘 | Tsukahara H Hata I Sekine K | 1998 | Early Hum Dev1998，51，1： | 1 |
| 15 | Development of a frameless and armless stereotactic neuronavigation system with ultrasonographic registion 显示文摘 | Hata N Dohi T Iseki H | 1997 | Neurosurgery1997，41，3： | 1 |
| 16 | Perianal Paget's disease treated with a wide excision and gluteal fold flap reconstruction guided by photodynamic diagnosis:report of a case显示文摘 | Araki Y Noake T Hata H | 2003 | Dis Colon Rectum2003，46，11： | 1 |
| 17 | The role of NF-kappa B in retinal neovascularization in the rat:Possible involvement of cytokine-induced neutrophil che-moattractant(CINC),a member of the interleukin-8 family显示文摘 | Yoshida A Yoshida S Hata Y Khalil AK Ishibashi T Inotoma H | 1998 | J Histochem Cyto Ckem1998，46，10： | 1 |
| 18 | Plasma brain natriuretic peptide as a novel therapentic indicator in idiopathic dilated cardiomyopathy during β-blocker therapy:A potential of hormone-guided treatment显示文摘 | Hata K Tataok H | 2001 | Am Heart J2001，141，： | 1 |
| 19 | Altered expression of amylold precursors proteins after traumatic brain injury in rats:in situ hybridization and immunohistochemical study 显示文摘 | Masumura M Hata R Uramoto H | 2000 | J Neurotrauma2000，17，： | 1 |
| 20 | Inhibition of prokiferative responses of mouse spleen lymphocytes and rabbit Peyer's patch cells by bovine milk caseins and their digests 显示文摘 | Orani H Hata I | 1995 | Journal of Dairy Research1995，62，： | 1 |
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