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| 1 | Preparation and Characterization of Poly Lactic Acid/Graphene Oxide/Nerve Growth Factor Scaffold with Electrical Stimulation for Peripheral Nerve Regeneration in vitro显示文摘A novel conductive drug-loading system was prepared by using an improved emulsion electrostatic spinning method which contained polylactic acid (PLA),graphene oxide (GO),and nerve growth factor (NGF) coated with bovine serum albumin (BSA) nanoparticles.Firstly,the structure,mechanical properties,morphology and electrical conductivity of PLA/GO electro spun fiber membranes with different GO ratios were characterized.PLA/GO scaffolds can exhibit superior porosity,hydrophilic and biomechanical properties when the GO incorporation rate is 0.5%.The addition of GO in the PLA/GO electro spun fiber membranes can also create appropriate pH environment for the repair of injured nerve when the GO incorporation rate is above 0.5%.Secondly,PLA/GO/BSA/Genipin/NGF particles (with a ratio of BSA/NGF=3:1) prepared by modified emulsion electro spinning method will release more NGF than PLA/GO/NGF particles.In addition,PLA/0.5%GO/NGF scaffold can maintain its structure stability for at least 8 weeks observed by scanning electron microscope (SEM).Moreover,the degradation of PLA/0.5%GO/NGF scaffold is consistent with its weight loss.Finally,in vitro assay confirmes that PLA/GO composite scaffold exhibits low cytotoxicity to RSC96 cells.Cellular results have demonstrated that PLA/0.5%GO/NGF sustained-release drug sustained-release system with appropriate electrical stimulation (ES) can promote PC12 cell proliferation,and it can maintain its differentiation capability for at least 3 weeks.In conclusion,PLA/0.5%GO/NGF sustained-release drug sustained-release system can maintain its biological activity for at least 3 weeks and promote cell proliferation with appropriate ES. | 徐海星 LI Rui LI Yiping HE Qundi YAN Xiumei SHU Tao YANG Haixia LÜ Yifei LI Zheng XU Runtian 熊承杰 许沛虎 | 2020 | Journal of Wuhan University of Technology(Materials Science)2020，35，6： | 2 |
| 2 | The Study of Nerve Conduit with Biocompatibility and Electrical Stimulation Effect显示文摘Conductive polymers show great promise because of their electrical property based on bioelectricity in vivo. In order to search electro-activity materials which insure abduction of tissue, we synthesized conductive nerve conduits with poly-dl-lactic-acid(PDLLA) and tetra-aniline(TA). Preparation technology of TA was optimized, and the properties of the conduit were studied. RSC96 cell were used to investigate the toxicity and electrical stimulation effect. SD rats were used to assess biocompatibility in vivo. The results showed that the reaction ratio of 1:1, the reaction time of 2 h and the HCl concentration of 2 mol/L were the optimum conditions for synthesis of TA. The influence of TA content on the mechanical properties, hydrophilicity, conductivity and microstructure of the nerve conduit was evaluated. Cell and histocompatibility study showed PDLLA/TA possessed good biocompatibility. These results showed it had application values in tissue projects. | 郭兴蕾 刘刚 HE Qundi 徐海星 YU Yunxuan KE Changsheng MING Xiaofei ZHAO Meng WANG Xiaobin HUANG Zhijun 许沛虎 | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，6： | 1 |
| 3 | PDLLA/β-TCP/HA/CHS/NGF Sustained-release Conduits for Peripheral Nerve Regeneration显示文摘Using nerve guide conduits (NGCs) to promote the regeneration of PNI is a feasible alternative to autograft.Compared with NGCs made of single material,composite NGCs have a greater development prospect.Our previous research has confirmed that poly(D,L-lactic acid)/β-tricalcium phosphate/hyaluronic acid/chitosan/nerve growth factor (PDLLA/β-TCP/HA/CHS/NGF) NGCs have excellent physical and chemical properties,which can slowly release NGF and support cell adhesion and proliferation.In this study,PDLLA/β-TCP/HA/CHS/NGF NGCs were prepared and used to bridge a 10 mm sciatic nerve defect in 200-250 g Sprague-Dawley (SD) rat to verify the performance of the NGCs in vivo.Substantial improvements in nerve regeneration were observed after using the PDLLA/β-TCP/HA/CHS/NGF NGCs based on gross post-operation observation,triceps wet weight analysis and nerve histological assessment.In vivo studies illustrate that the PDLLA/β-TCP/HA/CHS/NGF sustained-release NGCs can effectively promote peripheral nerve regeneration,and the effect is similar to that of autograft. | 闫秀美 WANG Jing HE Qundi 徐海星 TAO Junyan KORAL Kelly LI Kebi XU Jingyi WEN Jing HUANG Zhijun 许沛虎 | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，4： | 0 |
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