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| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Use of zeolitized coal fly ash in the simultaneous removal of ammonium and phosphate from aqueous solution显示文摘Discharge of wastewater containing nitrogen and phosphate can cause eutrophication.Therefore,the development of an efficient material for the immobilization of the nutrients is important.In this study,a low calcium fly ash and high calcium fly ash were converted into zeolite using the hydrothermal method.The removal of ammonium and phosphate that coexist in aqueous solution by the synthesized zeolites were studied.The results showed that zeolitized fly ash could efficiently eliminate ammonium and phosphate at the same time.Saturation of zeolite with Ca^(2+)rather than Na+favored the removal of both ammonium and phosphate because the cation exchange reaction by the NH_(4)^(+)resulted in the release of Ca2+into the solution and precipitation of Ca^(2+)with PO_(4)^(3−)followed.An increase in the temperature elevated the immobilization of phosphate whereas it abated the removal of ammonium.Nearly 60%removal efficiency for ammonium was achieved in the neutral pH range from 5.5 to 10.5,while the increase or decrease in pH out of the neutral range lowered the adsorption.In contrast,the removal of phosphate approached 100%at a pH lower than 5.0 or higher than 9.0,and less phosphate was immobilized at neutral pH.However,there was still a narrow pH range from 9.0 to 10.5 favoring the removal of both ammonium and phosphate.It was concluded that the removal of ammonium was caused by cation exchange;the contribution of NH3 volatilization to immobilization at alkaline conditions(up to pH level of 11.4)was limited.With respect to phosphate immobilization,the mechanism was mainly the formation of precipitate as Ca_(3)(PO_(4))_(2) within the basic pH range or as FePO_(4) and AlPO_(4) within acidic pH range. | WU Deyi HU Zhanbo WANG Xinze HE Shengbing KONG Hainan | 2007 | Frontiers of Environmental Science & Engineering2007，1，2： | 2 |
| 3 | Variation of phytoplankton community before an induced cyanobacterial(Arthrospira platensis) bloom显示文摘A cyanobacterial (Arthrospira platensis) bloom was induced in situ by nutrient manipulation in an enclosure. The succession of the phytoplankton community and the water chemistry variations before the appearance of bloom, as well as their relationship, were investigated. The cell pigment variations were studied simultaneously. The Pearson's correlation analysis showed that there was no significant correlation between water chemistry and green algal or cyanobacterial composition, indicating that water chemistry variations were not suitable to be used as indicators for cyanobacterial-bloom early-warning. However, the diversity index of the phytoplankton community decreased sharply before the bloom appeared. Therefore, the dynamics of phytoplankton community was put forward to be an indicator for cyanobacterial-bloom early-warning. In addition, the cell pigment variations represented the changes of community structure, which should be useful for studying the dynamics of phytoplankton community. | WANG Chong, HUANG Yingying, HE Shengbing, LIN Yan, WANG Xinze, KONG Hainan School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. | 2009 | Journal of Environmental Sciences2009，21，12： | 2 |
| 4 | Controlled observation of posthemiplegic omalgia treated by acupuncture-moxibustion and rehabilitation显示文摘 | HE Xinze DAI Shuqing SU Li | 2012 | World Journal of Aeupuncture-Moxibus- tion2012，22，4： | 1 |
| 5 | Crop breeding and bioteehnology in China显示文摘 | XinZ Y He Z H Ma Y Z Zhuang Q S | 1999 | Journal of New Seeds1999，1，1： | 1 |
| 6 | Mechanical and environmental properties of geopolymer-stabilized domestic waste incineration slag in an asphalt pavement base显示文摘Domestic waste incineration slag(WIS)includes fly ash and slag.Fly ash is classified as hazardous waste because it contains heavy metals.Most of slag are directly stacked or landfilled due to problems such as large output and low utilization rate.Harmless treatment is imminent.If WIS is used effectively in the road engineering,which can realize the high-quality and high-efficiency recycling of WIS,and it is of great significance to save resources and protect the environment.This study applies a geopolymer prepared from WIS fly ash as a stabilizing agent in WIS blending macadam for use as a pavement base mixture,and reports the mechanical properties(unconfined compressive strength,splitting strength,and resilience modulus)of the geopolymer-stabilized WIS blending macadam(GeoWIS).The leaching concentrations of harmful heavy metals of GeoWIS soaked in water were also investigated.Finally,the strength formation and heavy metal stability mechanisms were explored.The unconfined compressive strength,splitting strength,and compressive resilient modulus of GeoWIS all increased with increasing geopolymer content and decreasing WIS content.The strength of GeoWIS was derived from its geopolymerization and hydration products(C-S-H gel,N-A-S-H gel,and AFt).When the geopolymer content reached 12%–14%,the GeoWIS without natural macadam met the strength criterion of the asphalt pavement base.Through physical adsorption and chemical bonding,the geopolymer significantly reduced the leaching of harmful heavy metals.In GeoWIS with 50%WIS and stabilized with 10%geopolymer,the Cr,Ni,Cd,and Pb concentrations met the grade III groundwater standard.Concentrations of heavy metals leached from GeoWIS are low and exert little impact on environment. | Xiaoping Ji Bo Chen Xinze Dong Honglei Lu Xueyuan Zhang Shupeng He Tongda Wu | 2023 | Journal of Road Engineering2023，3，2： | 0 |
| 7 | Flexible multichannel electrodes for acute recording in nonhuman primates显示文摘Flexible electrodes have demonstrated better biocompatibility than rigid electrodes in relieving tissue encapsulation and long-term recording.Nonhuman primates are closer to humans in their brains’structural and functional properties,thus making them more suitable than rodents as animal models for potential clinical usage.However,the application of flexible electrodes on nonhuman primates has rarely been reported.In the present study,a flexible multichannel electrode array for nonhuman primates was developed and implemented for extracellular recording in behaving monkeys.To minimize the window of durotomy for reducing possible risks,a guide-tube-compatible implantation solution was designed to deliver the flexible electrodes through the dura into the cortex.The proposed structure for inserting flexible electrodes was characterized ex vivo and validated in vivo.Furthermore,acute recording of multichannel flexible electrodes for the primates was performed.The results showed that the flexible electrodes and implantation method used in this study meet the needs of extracellular recording in nonhuman primates.Task-related neuronal activities with a high signal-to-noise ratio of spikes demonstrated that our whole device is currently a minimally invasive and clinically viable approach for extracellular recording. | Yang Wang Qifan Wang Ruichen Zheng Xinxiu Xu Xinze Yang Qiang Gui Xiaowei Yang Yijun Wang He Cui Weihua Pei | 2023 | Microsystems & Nanoengineering2023，9，4： | 0 |
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