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| 1 | Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one- compound peptide libraries显示文摘one-bead-one-compound (OBOC ) 组合的肽图书馆是一个强大的工具识别 ligand 和受体相互作用。这里,我们使用了 OBOC 图书馆技术识别对主要贝壳变应原 tropomyosin 的免疫球蛋白 E (IgE ) epitopes 特定的 mimotopes。有 8-12 氨基酸残余的 OBOC 肽图书馆为 tropomyosin 的 IgE mimotopes 与贝壳过敏症从病人与浆液样品被屏蔽。25 mimotopes 从屏蔽被识别,他们到 tropomyosin 特定的 IgE 的有约束力的反应被肽 ELISA 证实。这些 mimotopes 能基于顺序相同被划分成七簇,并且由聚类的 mimotopes 的 EpiSearch 印射的 epitope 被执行描绘并且证实 mimotopes 的有效性。五从六预言的 epitopes 被发现与 tropomyosin 的以前识别的 epitopes 重叠。为了推进,证实 mimotopes 的模仿潜力, BALB/c 老鼠被使免疫, mimotopes 结合了到锁眼恋栈者 hemocyanin 和 assayed 让他们的能力导致 tropomyosin 特定的抗体。那收到的 mimotope 免疫被发现有 tropomyosin 特定的免疫球蛋白 G 的提高的水平的 BALB/c 老鼠,然而并非收到了无关的 mimotope 的老鼠。这研究用整个 sera 开创 OBOC 图书馆的成功的申请屏蔽并且识别多重虾变应原 mimotopes 并且验证他们在 vitro 使用的模仿潜力,在 vivo,并且在 silico 方法。 | Nicki YH Leung Christine YY Wai Marco HK Ho Ruiwu Liu Kit S Lam Jin Jun Wang Shang An Shu Ka Hou Chu Patrick SC Leung | 2017 | Cellular & Molecular Immunology2017，14，3： | 6 |
| 2 | Copper transportion of WD protein in hepatocytes from Wilson disease patients in vitro显示文摘AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD copper transporting properties in some organelles of the cultured hepatocytes were studied from WD patients and normal controls. These cultured hepatocytes were incubated in the media of copper 15mg.L-1 only, copper 15 mg. L-1 with vincristine (agonist of P-type ArPase) 0.5mg. L-1, or copper 15 mg. L-1 withvanadate (antagonist of P-type ATPase) 18.39 mg. L-1separately. Microsome (endoplasmic reticulum and Golgi apparatus), lysosome, mitochondria, and cytosol were isolated by differential centrifugation. Copper contents in these organelles were measured with atomic absorption spectrophotometer, and the influence in copper transportion of these organelles by vanadate and vincristine were comparatively analyzed between WD patients and controls.WD copper transporting P-type ATPase was detected by SDS-PAGE in conjunction with Western blot in liver samples of WD patients and controls.RESULTS: The specific WD proteins (Mr155 000 lanes) were expressed in human hepatocytes, including the control and WD patients. After incubation with medium containing copper for 2 h or 24 h, the microsome copper concentration in WD patients was obviously lower than that of controls,and the addtion of vanadate or vincristine would change the copper transporting of microsomes obviously. When incubated with vincristine, levels of copper in microsome were significantly increased, while incubated with vanadate,the copper concentrations in microsome were obviously decreased. The results indicated that there were Wdproteins, the copper transportion P-type ATPase in the microsome of hepatocytes. WD patients possessed abnormal copper transporting function of WD protein in the microsome, and the agonist might correct the defect of copper transportion by promoting the activity of copper transportion P-type ATPase.CONCLUSION: Copper transportion P-type ATPase plays an important role in hepatocytic copper metabolism.Dysfunction of hepatocytic WD protein copper transportion might be one of the most important factors for WD. | Guo-Qing Hou~1 Xiu-Ling Liang~2 Rong Chen~2 Li-Wen Tang~3 Ying Wang~2 Ping-Yi Xu~2 Ying-Ru Zhang~2 Cui-Hua Ou~2 1 Department of Neurology.Guangzhou First Municipal People’s Hospital,Guangzhou Medical College,Guangzhou 510180,Guangdong Province.China2 Department of Neurology.First Affiliated Hospital.Sun Yat-Sen University of Medical Sciences.Guangzhou 510080.Guangdong Province.China3 Department of Pharmacology,University of Kentucky.Lexington,KY 40506.USA | 2001 | World Journal of Gastroenterology2001，7，6： | 4 |
| 3 | Enhancement of Extracts from Celastrus orbiculatus on Maturation and Function of Dendritic Cells in vitro and in vivo显示文摘Objective To examine the immunoregulation of Celastrus orbiculatus extracts (COE), a traditional Chinese medicine, on maturation and function of dendritic cells (DCs) in vitro and in vivo. Methods In vitro, after treated with COE in different nontoxic concentrations (0, 10, 20, 40, 80, and 160 μg/mL) for 5 d, the surface immunological molecules and cytokine secretion of mice bone marrow-derived DCs in response to COE were analyzed by flow cytometric analysis (FACS) and enzyme linked immunosorbent assay (ELISA), respectively. In vivo, mouse hepatoma cells (Hepa1-6, 1 × 106) were injected sc and were treated with different dosages of COE (10, 20 or 40 mg/kg/d). Effects on tumor growth were determined by tumor volume and histology analysis after 28 d administration of COE. The relative proportions of mature DCs and CD8+ T cells were measured in mononuclear cells that had been isolated from spleen by FACS. Results COE stimulated IL-2 and IFN-γ secretion of DCs, simultaneously enhanced the maturation of DCs by enhancing immunological molecule (CD40, CD80, CD86, H-2Kb, and I-Ab) expression in a dose-dependent manner. Furthermore, the chemotactic responses of DCs were significantly higher in COE-treated than untreated DCs, in association with higher chemokine receptor 7 expression. Furthermore, COE increased DCs produce IFN-γ and IL-2 in a dose-dependent manner when the concentration of COE less than 40 μg/mL, decreased DCs produce IL-10 and IL-4 also in a dose-dependent manner. In in vivo studies, COE can not only suppress growth of malignant hepatocellular carcinomas but also stimulate maturation of DCs, associated with strongly enhanced CD8+ CTL responses. Conclusion These data provide new insight into the mechanism of action of COE and indicate that the stimulation of maturation and function of DCs by COE contributes to its immunoregulatory effects. | QIAN Ya-yun1, ZHANG Hua2, YUAN Lin1,3, HOU Ying1, LIU Wei-wei1, LIU Yan-qing1 1. Institute of Traditional Chinese Medicine & Western Medicine, Yangzhou University, Yangzhou 225009, China 2. Department of nursing, Medical College, Yangzhou University, Yangzhou 225009, China 3. Department of Image, 97th Hospital of People’s Liberation Army, Xuzhou 221000, China | 2010 | Chinese Herbal Medicines2010，2，3： | 4 |
| 4 | Effectiveness of intravenous r- tPA versus UK for acute ischaemic stroke: a nationwide prospective Chinese registry study显示文摘Background Intravenous recombinant tissue plasminogen activator(r-tPA)and urokinase(UK)are both recommended for the treatment of acute ischaemic stroke(AIS)in China,but with few comparative outcome data being available.We aimed to compare the outcomes of these two thrombolytic agents for the treatment of patients within 4.5 hours of onset of AIS in routine clinical practice in China.Methods A pre-planned,prospective,nationwide,multicentre,real-world registry of consecutive patients with AIS(age≥18 years)who received r-tPA or UK within 4.5 hours of symptom onset according to local decision-making and guideline recommendations during 2017-2019.The primary effectiveness outcome was the proportion of patients with an excellent functional outcome(defined by modified Rankin scale scores 0 to 1)at 90 days.The key safety endpoint was symptomatic intracranial haemorrhage according to standard definitions.Multivariable logistic regression was used for comparative analysis,with adjustment according to propensity scores to ensure balance in baseline characteristics.Results Overall,4130 patients with AIS were registered but 320 had incomplete or missing data,leaving 3810 with available data for analysis of whom 2666 received r-tPA(median dose 0.88(IQR 0.78-0.90)mg/kg)and 1144 received UK(1.71(1.43-2.00)×104 international unit per kilogram).There were several significant intergroup differences in patient characteristics:r-tPA patients were more educated,had less history of stroke,lower systolic blood pressure,greater neurological impairment and shorter treatment times from symptom onset than UK patients.However,in adjusted analysis,the frequency of excellent outcome(OR 1.18,95%CI 1.00 to 1.40,p=0.052)and symptomatic intracranial haemorrhage(OR 0.70,95%CI 0.33 to 1.47,p=0.344)were similar between groups.Conclusions UK may be as effective and carry a similar safety profile as r-tPA in treating mild to moderate AIS within guidelines in China.Registration http://www.clinicaltrials.gov.unique identifier:NCT02854592. | Xinhong Wang Xiaoqiu Li Yuming Xu Runhui Li Qingcheng Yang Yong Zhao Fengyun Wang Baoying Sheng Runqing Wang Shaoyuan Chen Lihua Wang Liying Shen Xiaowen Hou Yu Cui Duolao Wang Bin Peng Craig S Anderson Huisheng Chen On behalf of INTRECIS Investigators | 2021 | Stroke & Vascular Neurology2021，6，4： | 2 |
| 5 | Cyclic rapid warming on centennial-scale revealed by a 2650-year stalagmite record of warm season temperature 显示文摘 | Tan M Liu T S Hou J | 2003 | Geophysical Research Letters2003，30，： | 2 |
| 6 | A comprehensive resource management framework for next generation wireless networks显示文摘 | Ye Jian Hou Jiongkuan Papavassiliou S | 2002 | IEEE Transactions on Mobile Computing2002，1，4： | 2 |
| 7 | Isolated olistoliths from the Lower Triassic Xikou Formation in southwestern Fujian and its geological significance显示文摘ISOLATED olistoliths refer to massive blocks of rock occurring out of place, but with sedimentary contact relationship, within unrelated sediments. They were transported as single masses into the basins in which they occurred. In this note we will first report examples found from the Lower Triassic Xikou Formation in southwestern Fujian and discuss its geological significance. | Li, PJ Hou, QL Li, JL Sun, S | 1997 | Chinese Science Bulletin1997，42，2： | 2 |
| 8 | Prediction of impact damage in composite plates显示文摘 | Hou J P Petrinic N Ruiz C Hallett S R | 2006 | Composites Science and Technology2006，60，： | 1 |
| 9 | MicroPET Imaging of Breast Cancer αv-Integrin Expression With ^84Cu-Labeled Dimeric RGD Peptides 显示文摘 | Chen X Y Liu S Hou Y P | 2004 | Mol Imag Biol2004，6，5： | 1 |
| 10 | The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings显示文摘 | WANG CH ZHANG S H WANG P F HOU J ZHANG W J LI W LIN ZH P | 2009 | Chemosphere2009，75，： | 1 |
| 11 | Transcriptional activity among high and low risk human papillomavirus E2 proteins correlates with E2 DNA binding 显示文摘 | Hou S Y Wu S Y Chiang C M | 2002 | J Biol Chem2002，277，45： | 1 |
| 12 | A Genetic Algorithm for Multiprocessor Scheduling显示文摘 | Hou E S H | 1994 | IEEE Transactions on Parallel and Distributed Systems1994，5，2： | 1 |
| 13 | The pathogenesis and clinical significance of a high-intensity zone(HIZ)of lumbar intervertebral disc on MR imaging in the patient with discogenic low back pain显示文摘 | Peng B Hou S Wu W | 2006 | Eur Spine2006，15，5： | 1 |
| 14 | Effect of SO2 on V2O5/ ACF catalysts for NO reduction with NH3 at Low temperature显示文摘 | Hou Y Q Huang Z G Guo S J | 2009 | Catal Commun2009，10，11： | 1 |
| 15 | Roles of epithelial cell derived periostin in TGF beta activa- tion,collagen production, and collagen gel elasticity in asthma显示文摘 | Sidhu S S Yuan S Innes A L Kerr S Woodruff P G Hou L | 2010 | Proc Natl Acad Sci USA2010，107，14： | 1 |
| 16 | A robot with improved absolute positioning accuracy for CT guided stereotactic brain surgery显示文摘 | KWOH Y S HOU J JONCKHEERE E A | 1988 | IEEE Transactions on Biomedical Engineering1988，35，2： | 1 |
| 17 | Effect of honey on naringin absorption from a decoction of the pericarps of Citrus grandis显示文摘 | Hou Y C Hsiu S L Yen H F | 2000 | Planta Med2000，66，5： | 1 |
| 18 | The Jak/STAT pathway in model organisms: emerging roles in cell movement 显示文摘 | Hou S X Zheng Z Chen X | 2002 | Dev Cell2002，3，6： | 1 |
| 19 | On network bandwidth allocation policies and feedback control algorithms for packet networks显示文摘 | Y T HOU B LI S S PANWAR | 2000 | Computer Networks2000，34，3： | 1 |
| 20 | On-Line Monitoring of Formaldehyde in Air Using Chemiluminescence Detection显示文摘 | Song Z H Hou S | 2003 | International Journal of Environmental Analytical Chemistry2003，83，9： | 1 |
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