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| 1 | Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs by leucine through an mTORC1-dependent pathway显示文摘Neonatal growth is characterized by a high protein synthesis rate that is largely due to an enhanced sensitivity to the postprandial rise in insulin and amino acids, especially leucine. The mechanism of leucine's action in vivo is not well understood. In this study, we investigated the effect of leucine infusion on protein synthesis in skeletal muscle and liver of neonatal pigs. To evaluate the mode of action of leucine, we used rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) complex-1 (mTORC1). Overnight-fasted 7-day-old piglets were treated with rapamycin for 1 hour and then infused with leucine (400 μmol·kg -1 ·h -1 ) for 1 hour. Leucine infusion increased the rate of protein synthesis, and ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) phosphorylation in gastrocnemius and masseter muscles (P < 0.05), but not in the liver. The leucine-induced stimulation of protein synthesis and S6K1 and 4E-BP1 phosphorylation were completely blocked by rapamycin, suggesting that leucine action is by an mTORC1-dependent mechanism. Neither leucine nor rapamycin had any effect on the activation of the upstream mTORC1 regulators, AMP-activated protein kinase and protein kinase B, in skeletal muscle or liver. The activation of eIF2a and elongation factor 2 was not affected by leucine or rapamycin, indicating that these two pathways are not limiting steps of leucine-induced protein synthesis. These results suggest that leucine stimulates muscle protein synthesis in neonatal pigs by inducing the activation of mTORC1 and its downstream pathway leading to mRNA translation. | Agus Suryawan Hanh V Nguyen Rosemarie D Almonaci Teresa A Davis | 2012 | Journal of Animal Science and Biotechnology2012，3，1： | 9 |
| 2 | Effect of selection for plasma corticosterone level and feed conversion ration in dwarf White Rocks and adaptability to hot tomperalum during growth显示文摘 | Pingel H Hallu C Hanh D T | 1995 | Arch Tierz1995，38，： | 1 |
| 3 | Methanolysis of Triolein by Low Frequency Ultrasonic Irradiation 显示文摘 | Hanh H D Dong N T Starvarache C | 2008 | Energy Conversion and Management2008，49，： | 1 |
| 4 | Amino acid composition of the milk of some mammalian species changes with stage of lactation 显示文摘 | Teresa A D Hanh V N Roselina G B | 1994 | British Journal of Nutrition1994，72，： | 1 |
| 5 | A comparison of ELISA and f low microsplere-based assays for quanti f ication of immunoglobulins显示文摘 | Joseph D Juliet L Hanh B | 2002 | J Immunol Methods2002，263，2： | 1 |
| 6 | Trajectory control of electro-hydraulic excavator using fuzzy self tuning algorithm with neural network显示文摘 | HANH L D AHN K K KHA N B | 2009 | Journal of Mechanical Science and Technology2009，，23： | 1 |
| 7 | Development of a comprehensive analytical method for semi-volatile organic compounds in sediments by using an automated identification and quantification system with a GC-MS database显示文摘 | Kadokam I K Pan S Hanh D | 2012 | Analytical Sciences2012，28，12： | 1 |
| 8 | On the axiomatics of Ann-categories显示文摘 | Quang N T Hanh D D Thuy N T | 2008 | Journal of Algebra Number Theory and Ap plications2008，11，1： | 1 |
| 9 | Salt tolerance of glycine- betaine-deficient and-containing maize lines显示文摘 | Saneoka H Nagasaka C Hanh D T | 1995 | Plant Physiology1995，107，： | 1 |
| 10 | Satellite observation of coal mining subsidence by persistent scatterer analysis显示文摘 | HANH C J SANG W K HYUNG S J KYUNG D M JOONG S W | 2007 | Engineering Geology2007，92，12： | 1 |
| 11 | Methanolysis of triolein by low frequency ultrasonic irradiation显示文摘 | Hanh H D Dong N T Starvarache C | 2008 | Energy Conversion and Management2008，49，2： | 1 |
| 12 | A retrospective analysis to determine if the timing of H&D curve production has a clinically significant effect on the percent difference in agreement of isodose delivery for film-based IMRT QA显示文摘 | Mahsa D Hanh P | 2004 | Med Dosim2004，29，2： | 1 |
| 13 | Methanolysis of triolein by low frequency ultrasonic irradiation显示文摘 | Hanh H D Dong N T Starvarache C | 2008 | Energy Conversion and Management2008，49，2： | 1 |
| 14 | Methanolysis of triolein by low frequency ultrasonic irradiation显示文摘 | Hanh H D Dong N T Starvarache C | 2008 | Energy Conversion and Management2008，，49： | 1 |
| 15 | Liquid culture as a positive condition to induce and enhance quality and quantity of somatic embryogenesis of Lilium longiflorum 显示文摘 | Nhut D T Hanh N T M Tuan P Q Nguyet L T M Tram N T H Chinh N C Nguyen N H Vinh D N | 2006 | Scientia Horticulturae2006，110，1： | 1 |
| 16 | Liquid culture as a positive condition to induce and enhance quality and quantity of somatic embryogenesis of Lilium longiflorum显示文摘 | Nhut D T Minh Hanh N T | 2006 | Scientia Horticulturae2006，110，： | 1 |
| 17 | A comparison of ELISA and flow microsphere-based assays for quantification of immunoglobulins显示文摘 | Joseph D Juliet L Hanh B | 2002 | J Immunol Meth2002，263，： | 1 |
| 18 | Pharmaceutical applications of photothermal beam deflection显示文摘 | Hanh B D Faubel W Heissler S | 2006 | Laser Physics2006，16，5： | 1 |
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