| 2 | Induction of osteoblast differentiation in human adipose derived stem cells by lanthanum ions显示文摘Adipose derived stem cells represent a readily available source of adult stem cells for various biomedical applications. In this study, the proliferation and osteogenic differentiation potential of lanthanum nitrate(La3+) on human adipose derived mesenchymal stem cells(hADSCs) were investigated for the first time and compared with that of dexamethasone(Dex). Our results provided evidence that La3+ at 50 μmol/L concentration promoted proliferation of hADSCs upto 2.4 fold when treated for 21 d in DMEM medium. Treatment of hADSCs with La3+ containing osteogenic induction medium(α-MEM with ascorbic acid and β-glycerophosphate) for 7 d resulted in higher calcium deposition than that in the presence of Dex(0.1 μmol/L) as shown by Alizarin red S and von Kossa staining. Scanning electron micrographs also showed more extracellular matrix mineralization in the presence of La3+. After 7 d of treatment with La3+(10 μmol/L) the expression of RunX2, osteopontin(OP) and osteocalcin(OC) increased 3.4, 5.5 and 2.7 fold respectively. Our results provided evidence that in the presence of La3+ osteogenic differentiation occurred earlier than that in the presence of Dex. | Harini D Indra R Rajaram A Rama Rajaram | 2014 | Journal of Rare Earths2014，32，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员