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| 1 | Extrasynaptic and synaptic NMDA receptors form stable and uniform pools in rat hippocampal slices显示文摘 | Harris AZ Pettit DL | 2007 | J Phys- iol2007，584，： | 1 |
| 2 | Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdcl4A phosphatase 显示文摘 | Brett KK Zachary AZ Harry C | 2002 | Mol Biol Cell2002，13，7： | 1 |
| 3 | Extrasynaptie and synaptic NMDA receptors form stable and uniform pools in rat hippocampal slices 显示文摘 | Harris AZ Pettit DL | 2007 | J Physiol2007，584，2： | 1 |
| 4 | Postoperative Cognitive Dysfunction in Middle-aged Patients显示文摘 | Tim Johnson Terri Monk Lars S. Rasmussen Hanne Abildstrom Peter Houx Kari Korttila Harrie M. Kuipers Chris D. Hanning Volkert D. Siersma Diana Kristensen Jaume Canet Maria Teresa Iba?az Jakob T. Moller | 2002 | Anesthesiology2002，，6： | 1 |
| 5 | Evidence of a feedback mechanism limiting plant response to elevated carbon dioxide 显示文摘 | DíAZ S GRIME J P HARRIS J | 1993 | nature1993，364，： | 1 |
| 6 | Crohn’s disease: Influence of age at diagnosis on site and clinical type of disease显示文摘 | JM Polito B Childs ED Mellits AZ Tokayer ML Harris TM Bayless | 1996 | Gastroenterology1996，，： | 1 |
| 7 | Kecruiting extrasynaptic NMDA recep- tors augments synaptic signaling显示文摘 | Harris AZ Pettit DL | 2008 | J Neurophysiol2008，99，2： | 1 |
| 8 | Helicobacter pylori vacA s1a and s1b alleles from clinical isolates from different regions of Chile show a distinct geographic distribution显示文摘AIM: To establish the most common vacA alleles in Helicobacter pylori(H pylori) strains isolated from Chilean patients and its relationship with gastritis and gastroduodenal ulcers.METHODS: Two hundred and forty five H pylori clinical isolates were obtained from 79 biopsies from Chilean infected patients suffering from gastrointestinal diseases. An average of 2-3 strains per patient was isolated and the vacA genotype was analyzed by PCR and 3% agarose electrophoresis. Some genotypes were checked by DNA sequencing.RESULTS: The most prevalent vacA genotype inChilean patients was s1b m1 (76%), followed by s1a m1 (21%). In contrast, the s2 m2 genotype was scarcely represented (3%).The s1b m1 genotype was found most frequently linked to gastropathies (P<0.05) rather than ulcers. Ulcers were found more commonly in male and older patients. Curiously, patients living in cities located North and far South of Santiago, the capital and largest Chilean city, carried almost exclusively strains with the s1b m1 genotype. In contrast, patients from Santiago and cities located South of Santiago carried strains with either one or both s1a m1 and s1b m1 genotypes.Regarding the s2 m2 genotype, comparison with GenBank sequences revealed that Chilean s2 sequence was identical to those of Australian, American, and Colombian strains but quite different from those of Alaska and India.CONCLUSION: Differences in geographic distribution of the s and m vaccA alleles in Chile and a relationship of s1b m1 genotype with gastritis were found. Sequence data in part support a hispanic origin for the vacA genotype.Asymmetric distribution of genotypes s1b m1 and s2 m2recedes H Pyloristrain distribution in Spain and Portugal. | MI Díaz A Valdivia P Martínez JL Palacios P Harris J Novales E Garrido D Valderrama C Shilling A Kirberg E Hebel J Fierro R Bravo F Siegel G Leon G Klapp A Venegas | 2005 | World Journal of Gastroenterology2005，11，40： | 1 |
| 9 | Recruiting extrasynaptie NMDA receptors augments synaptic signaling显示文摘 | Harris AZ Pettit DL | 2008 | J Neurophysiol2008，99，2： | 1 |
| 10 | Extrasynaptic and synaptic NMDA receptors form stable and uniform pools in rat hippocampal slices显示文摘 | Harris AZ Pettit DL | 2007 | J Physiol2007，584，2： | 1 |
| 11 | Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates显示文摘AIM:To evaluate how widely Helicobacter pylori (H. pylori ) HopE and HopV porins are expressed among Chilean isolates and how seroprevalent they are among infected patients in Chile.METHODS: H. pylori hopE and hopV genes derived from strain CHCTX-1 were cloned by polymerase chain reaction (PCR), sequenced and expressed in Escherichia coli AD494 (DE3). Gel-purified porins were used to prepare polyclonal antibodies. The presence of both genes was tested by PCR in a collection of H. pylori clinical isolates and their expression was detected in lysates by immunoblotting. Immune responses against HopE, HopV and other H. pylori antigens in sera from infected and non-infected patients were tested by Western blotting using these sera as f irst antibody on recombinant H. pylori antigens.RESULTS: PCR and Western blotting assays revealed that 60 and 82 out of 130 Chilean isolates carried hopE and hopV genes, respectively, but only 16 and 9, respectively, expressed these porins. IgG serum immunoreactivity evaluation of 69 H. pylori-infected patients revealed that HopE and HopV were infrequently recognized (8.7% and 10.1% respectively) compared to H. pylori VacA (68.1%) and CagA (59.5%) antigens. Similar values were detected for IgA serum immunoreactivity against HopE (11.6%) and HopV (10.5%) although lower values for VacA (42%) and CagA (17.4%) were obtained when compared to the IgG response.CONCLUSION: A scarce expression of HopE and HopV among Chilean isolates was found, in agreement with the infrequent seroconversion against these antigens when tested in infected Chilean patients. | Maritza Lienlaf Juan Pablo Morales María Inés Díaz Rodrigo Díaz Elsa Bruce Freddy Siegel Gloria León Paul R Harris Alejandro Venegas | 2010 | World Journal of Gastroenterology2010，16，3： | 0 |
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