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| 1 | Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis显示文摘AIM:To investigate the utility of immunohistochemical(IHC) staining with an antibody to Mycobacterium tuberculosis(M.tuberculosis) for the diagnosis of intestinal tuberculosis(TB).METHODS:We retrospectively identified 10 patients(4 males and 6 females;mean age = 65.1 ± 13.6 years) with intestinal TB.Clinical characteristics,including age,gender,underlying disease,and symptoms were obtained.Chest radiograph and laboratory tests,including sputum Ziehl-Neelsen(ZN) staining,M.tuberculosis culture,and sputum polymerase chain reaction(PCR) for tubercle bacilli DNA,as well as Tuberculin skin test(TST) and QuantiFERON-TB gold test(QFT),were examined.Colonoscopic records recorded on the basis of Sato's classification were also reviewed,in addition to data from intestinal biopsies examined for histopathological findings,including hematoxylin and eosin staining,and ZN staining,as well as M.tuberculosis culture,and PCR for tubercle bacilli DNA.For the present study,archived formalin-fixed paraffin-embedded(FFPE) intestinal tissue samples were immunohistochemically stained using a commercially available species-specific monoclonal antibody to the 38-kDa antigen of the M.tuberculosis complex.These sections were also stained with the pan-macrophage marker CD68 antibody.RESULTS:From the clinical data,we found that no patients were immunocompromised,and that the main symptoms were diarrhea and weight loss.Three patients displayed active pulmonary TB,six patients(60%) had a positive TST,and 4 patients(40%) had a positive QFT.Colonoscopic findings revealed that all patients had type 1 findings(linear ulcers in a circumferential arrangement or linear ulcers arranged circumferentially with mucosa showing multiple nodules),all of which were located in the right hemicolon and/or terminal ileum.Seven patients(70%) had concomitant healed lesions in the ileocecal area.No acid-fast bacilli were detected with ZN staining of the intestinal tissue samples,and both M.tuberculosis culture and PCR for tubercle bacilli DNA were negative in all samples.The histopathological data revealed that tuberculous granulomas were present in 4 cases(40%).IHC staining in archived FFPE samples with anti-M.tuberculosis monoclonal antibody revealed positive findings in 4 patients(40%);the same patients in which granulomas were detected by hematoxylin and eosin staining.M.tuberculosis antigens were found to be mostly intracellular,granular in pattern,and primarily located in the CD68 + macrophages of the granulomas.CONCLUSION:IHC staining with a monoclonal antibody to M.tuberculosis may be an efficient and simple diagnostic tool in addition to classic examination methods for the diagnosis of intestinal TB. | Yasushi Ihama Akira Hokama Kenji Hibiya Kazuto Kishimoto Manabu Nakamoto Tetsuo Hirata Nagisa Kinjo Haley L Cash Futoshi Higa Masao Tateyama Fukunori Kinjo Jiro Fujita | 2012 | World Journal of Gastroenterology2012，18，47： | 12 |
| 2 | Inhibition by berberine of cyclooxygenase - 2 transcriptional activity in human colon cancer cells显示文摘 | Hibiya Y Mutoh M | 1999 | J Ethnopharmacol1999，66，2： | 1 |
| 3 | 2000 nitrifying biofilm on a membrane Formation and characteristics of modified with positively-charged polymer chains 显示文摘 | Hibiya K Tsuneda S Hirata A | 2000 | Colloid and Surfaces (B: Biointerfaces)2000，18，： | 1 |
| 4 | Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applicable to high-strength nitrogenous wastewater treatment显示文摘 | Terada A Hibiya K Nagai J | 2003 | Journal of Bioscience and Bioengineering2003，95，2： | 1 |
| 5 | Simplified mod- eling of simultaneous reaction kinetics of carbon oxidation and nitrification in biofilm processes 显示文摘 | TSUNEDA S AURESENIA J HIBIYA K | 2004 | Engineering in Life Sci- ences2004，4，3： | 1 |
| 6 | Ceramic probe for measuring the thermal conductivity of an electrically conductivity by the transient hot wire method显示文摘 | SHIN N HIBIYA T | 1988 | Review of Scientific Instruments1988，59，12： | 1 |
| 7 | Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor显示文摘 | Hibiya K Terada A Tsuneda S | 2003 | Journal of Biotechnology2003，100，： | 1 |
| 8 | Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane aerated biofilm reactor显示文摘 | HIBIYA K TERADA A TSUNEDA S | 2003 | Journal of Biotechnology2003，100，： | 1 |
| 9 | Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane aerated biofilm reactor显示文摘 | Hibiya K Terada A Tsuneda S | 2003 | Journal of Biotechnology2003，100，1： | 1 |
| 10 | Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applica- ble to high strength nitrogenous wastewater treatment 显示文摘 | Terada A Hibiya K Nagai J | 2003 | Journal of Bioscience and Bioengineering2003，95，2： | 1 |
| 11 | Morphological changes in pneumocystis cariniiin the alveolar space due to treatment with co-trimoxazole-comparision of clinical cases and experimental rats显示文摘 | Hibiya I | 1990 | Kansenshogaku Zasshi1990，64，4： | 1 |
| 12 | In hibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells显示文摘 | Fukuda K Hibiya Y Mutoh M | 1999 | J Ethnopharmacol1999，66，2： | 1 |
| 13 | Inhibition by berberine of oydooxygonase - 2 tramscriptional activity in human colon cancer cells 显示文摘 | Fukuda K Hibiya Y Mutoh M | 1999 | J Ethrnophaumacol1999，66，2： | 1 |
| 14 | Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofihn reactor applicable to high-strength nitrogenous wastewater treatment 显示文摘 | Terada A Hibiya K Nagai J | 2003 | J Biosci Bioeng2003，95，2： | 1 |
| 15 | Linear stability of thermocapillary flow in partially confined half-zones显示文摘 | SUGURU SHIRATORI HENDRIK C KUHLMANN TAKETOSHI HIBIYA | 2007 | Physics of Fluids2007，19，： | 1 |
| 16 | Simul- taneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a mem- brane aerated biofilm reactor 显示文摘 | HIBIYA K TERADA A TSUNEDA S | 2003 | Journal of Biotech- nology2003，100，1： | 1 |
| 17 | Inhibition by berberine of cyclooxygenaae-2 transcriptional activity in humancolon cancer cells显示文摘 | Fukuda K Hibiya Y Mutoh M | | 0，，02： | 1 |
| 18 | Inhibition by berbefine of cyclooxygenase-2transcriptional activity in human colon cancer cells显示文摘 | Fukuda K Hibiya Y Mutoh M | 1999 | J Ethnopharmacol1999，66，2： | 1 |
| 19 | Formation and characteristics ofiai- trifying biofilm on a membrane modified with positively-charged- polymer chains显示文摘 | Hibiya K Tsuneda S Hirata A | 2000 | Colloid and Surfaces B:Biointerfaces2000，18，2： | 1 |
| 20 | Inhibition by berberine of cyclooxygenase 2 transcriptional activity in human colon cancer cells显示文摘 | FuKuda K Hibiya Y Mutoh M | 1999 | J Ethnopharmacol1999，66，2： | 1 |
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