| 1 | Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4显示文摘Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P > 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P < 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep. | Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 2 | Waste sorting and its effects on carbon emission reduction:Evidence from China显示文摘Considering the importance of waste sorting and treatment in the development of an ecological civilization,empirically evaluating the environmental impact of such programs is particularly important.This study uses Xiao'er Township in Gong County,Sichuan Province,China as a case study to analyze and estimate the carbon emission reduction effects of the township's pilot waste sorting program.Using the five-point sampling method,samples of waste are collected,reviewed,and measured for their major components and other key indicators.Additionally,questionnaire surveys and interviews are conducted in the township,along with investigations into existing records and other relevant information.The study adopts the solid waste management-greenhouse gas(SWM-GHG)calculator to study the township data.The case study results imply that proper waste sorting and treatment methods in villages and townships could play a major role in the reduction of carbon emission.Specifically,after implementing waste sorting in Xiao'er,annual carbon emissions were reduced by 2081 tons—equivalent to the electricity consumption of a family of three people for 1718 years,or the amount of CO_(2)emitted by 2641.6L vehicles driving once around the Earth.In the optimal scenario simulation,increasing the recycling of wet waste and recyclable waste further,the level of carbon emission reduction in Xiao'er could reach up to 4482 tons per year.According to the international general carbon trade price,this is equivalent to adding 44,820 US dollars to the GDP,or to an annual saving of 5.71 million kWh.If these waste management methods are expanded to villages and townships across China,then the carbon emissions reduced in a year would be equal to the CO_(2)emitted from electricity generation in Beijing for over a year.Based on these findings,this paper provides three policy recommendations for effective carbon emission reduction:increasing residents'environmental protection awareness over the long term,boosting funding support and enhancing the construction of supporting facilities,and strengthening governance and institutional capacity for waste sorting and treatment. | Hongping Lian Dechuan Wang Hui Li | 2020 | Chinese Journal of Population,Resources and Environment2020，18，1： | 0 |
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