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| 1 | Deeply learned broadband encoding stochastic hyperspectral imaging显示文摘Many applications requiring both spectral and spatial information at high resolution benefit from spectral imaging.Although different technical methods have been developed and commercially available,computational spectral cameras represent a compact,lightweight,and inexpensive solution.However,the tradeoff between spatial and spectral resolutions,dominated by the limited data volume and environmental noise,limits the potential of these cameras.In this study,we developed a deeply learned broadband encoding stochastic hyperspectral camera.In particular,using advanced artificial intelligenee in filter design and spectrum reconstruction,we achieved 7000-11,000 times faster signal processing and〜10 times improvement regarding noise toleranee.These improvements enabled us to precisely and dynamically reconstruct the spectra of the entire field of view,previously unreachable with compact computational spectral cameras. | Wenyi Zhang Hongya Song Xin He Longqian Huang Xiyue Zhang Junyan Zheng Weidong Shen Xiang Hao Xu Liu | 2021 | Light(Science & Applications)2021，10，6： | 4 |
| 2 | The egg cell is preferentially fertilized in Arabidopsis double fertilization显示文摘In flowering plants(angiosperms),fertilization of the egg cell by one sperm cell produces an embryo,whereas fusion of a second sperm cell with the central cell generates the endosperm.In most angiosperms like Arabidopsis,a pollen grain contains two isomorphic sperm cells required for this double fertilization process.A long-standing unsolved question is whether the two fertilization events have any preference.A tool to address this question is the usage of the cyclin-dependent kinase a1(cdka;1)mutant pollen,which produces a single sperm-like cell(SLC).Here,we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka;1 mutant pollen containing a single SLC.Single-cell RNA-sequencing analysis revealed that cdka;1 SLCs show a gene expression profile highly similar to that of sperm cells and not to the generative cell,precursor of the two sperm cells.Pollination assays using a limited number of cdka;1 mutant pollen revealed that in 98.2%of the ovules,single fertilization of the egg cell occurred.Pollination of pistils with excessive cdka;1 mutant pollen allowed the delivery of a second SLC via fertilization recovery,which fertilized the central cell,resulting in 20.7%double-fertilized ovules.This indicates that cdka;1 SLCs are able to fertilize both the egg and the central cell.Taken together,our findings have answered a long-standing question and support that preferential fertilization of the egg cell is evident in Arabidopsis. | Ling Li Saiying Hou Wei Xiang Zihan Song Yuan Wang Li Zhang Jing Li Hongya Gu Juan Dong Thomas Dresselhaus Sheng Zhong Li-Jia Qu | 2022 | Journal of Integrative Plant Biology2022，64，11： | 1 |
| 3 | Review of compact computational spectral information acquisition systems显示文摘With the development of computer science,more and more hardware implementations can be reproduced by software programming,bringing compact,cheap,and fast components to imaging instrumentation.In recent years,computational methods have been introduced into spectral detection,and computational spectrum acquisition implementations have emerged.This paper highlights the advantages of computational spectrum acquisition implementations by comparing them with traditional noncomputational methods.Then,focusing on the compact feature,we review the most representative implementations,and finally make discussion and offer an outlook. | Hongya SONG Wenyi ZHANG Haifeng LI Xu LIU Xiang HAO | 2020 | Frontiers of Information Technology & Electronic Engineering2020，21，8： | 0 |
| 4 | Lack of ethylene does not affect reproductive success and synergid cell death in Arabidopsis显示文摘The signaling pathway of the gaseous hormone ethylene is involved in plant reproduction,growth,devel-opment,and stress responses.During reproduction,the two synergid cells of the angiosperm female gametophyte both undergo programmed cell death(PCD)/degeneration but in a different manner:PCD/degeneration of one synergid facilitates pollen tube rupture and thereby the release of sperm cells,while PCD/degeneration of the other synergid blocks supernumerary pollen tubes.Ethylene signaling was postu-lated to participate in some of the synergid cell functions,such as pollen tube attraction and the induction of PCD/degeneration.However,ethylene-mediated induction of synergid PCD/degeneration and the role of ethylene itself have not been firmly established.Here,we employed the CRISPR/Cas9 technology to knock out the five ethylene-biosynthesis 1-aminocyclopropane-1-carboxylic acid oxidase(ACO)genes and created Arabidopsis mutants free of ethylene production.The ethylene-free mutant plants showed normal triple responses when treated with ethylene rather than 1-aminocyclopropane-1-carboxylic acid,but had increased lateral root density and enlarged petal sizes,which are typical phenotypes of mutants defective in ethylene signaling.Using these ethylene-free plants,we further demonstrated that production of ethylene is not necessarily required to trigger PCD/degeneration of the two synergid cells,but certain com-ponents of ethylene signaling including transcription factors ETHYLENE-INSENSITIVE 3(EIN3)and EIN3-LIKE 1(EIL1)are necessary for the death of the persistent synergid cell. | Wenhao Li Qiyun Li Mohan Lyu Zhijuan Wang Zihan Song Shangwei Zhong Hongya Gu Juan Dong Thomas Dresselhaus Sheng Zhong Li-Jia Qu | 2022 | Molecular Plant2022，15，2： | 0 |
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