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| 1 | Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation显示文摘IL-4 is an important B cell survival and growth factor. IL-4 induced the tyrosine phosphorylation of IRS2 in resting B lymphocytes and in LPS- or CD40L-activated blasts. Phosphorylated IRS2 coprecipitated with the p85 subunit of PI 3’ kinase in both resting and activated cells. By contrast, association of phosphorylated IRS2 with GRB2 was not detected in resting B cells after IL-4 treatment although both proteins were expressed. However, IL-4 induced association of IRS2 with GRB2 in B cell blasts. The pattern of IL-4- induced recruitment of p85 and GRB2 to IRS2 observed in B cells derived from STAT6 null mice was identical to that observed for normal mice. While IL-4 alone does not induce activation of MEK, a MEKI inhibitor suppressed the IL-4-induced proliferative response of LPS-activated B cell blasts. These results demonstrate that costimulation of splenic B cells alters IL-4-induced signal transduction independent of STAT6 leading to proliferation. Furthermore, proliferation induced by IL-4 in LPS-activated blasts is dependent upon the MAP kinase pathway. | ZAMORANO JOSE,Unidad de Investigacion, Hospital San Pedro de Alcantara, Avda Millan Astray, 10003 Caceres ANN E KELLY, JONATHAN AUSTRIAN, HELEN Y WANG, ACHSAH D KEEGAN (Department of Immunology, Jerome Holland Labs, American Red Cross, Rockville, MD, USA | 2001 | Cell Research2001，11，1： | 4 |
| 2 | Postpartum hemolytic uremic syndrome:a rare entity and a treatment challenge 显示文摘 | Alsina Segui E Martin Conde M Craver Hospital L Fernandez Oiraldez E | 2008 | Nefrologia2008，28，： | 1 |
| 3 | Molecular diagnosis of infective endocarditis by re- al-time broad-range polymerase chain reaction (PCR) and sequencing directly from heart valve tissue 显示文摘 | Marin M Mufioz P Sdnchez M del Rosal M Alcal6 L Rodrlguez-Cr6ixems M Bouza E Group for the Manage- ment of Infective Endocarditis of the Gregorio Marafi6n Hospital | 2007 | Medicine2007，86，4： | 1 |
| 4 | Simulating an opticalguidance system for the recovery of an unmanned underwatervehicle显示文摘 | Deltheil C Didier L Hospital E | 2000 | IEEE Journal of Oceanic Engineering2000，25，4： | 1 |
| 5 | New evidence of risk factors for community-acquired pneumonia: a population-based study 显示文摘 | Almirall J Bolibar I Serra-Prat M Roig J Hospital I Carandell E | 2008 | Eur Respir J2008，31，6： | 1 |
| 6 | Study of Tissue Engineered Dental Implants of human alveolar Bone-Evaluations on their osteogenic activities invitro显示文摘The development of the activated cellular bony implant, in light of the principle on tissue engineering, has brought about a new era to the fields of dental maxillofacial implantation. The present study separated the osteoblast like cells from human alveolar bone and seeded them into 3 types of biodegradable scaffold to form the complexes and then evaluated their osteogenic activities in vitro, in order to acquire experimental data that are essential to future clinical practice of this new type of therapeutical procedure in oral and maxillofacial surgery. Material and methods: Human alveolar bone origin cells were separated from alveolar bone around the third impacted teeth of 3 patients by enzyme digestion and went on cultures with α MEM containing β glycerophosphate and Dexamethasone at 5% CO2 ,37℃ for 21 28 days. Confirmed osteoblasts like cells were then seeded onto 3 types of degradable biomaterials of polyglycolic acid scaffold, collagen sponge, and L lactic acid/ε caprolactone to form cell matrices complexes. The 3 types of complex were continued to culture for 21 28 days in vitro at the same conditions with the single layer cultured cells. The cell proliferation, morphological changes, ALPase activity and mineral nodules formation on scaffolds were measured and observed at 3 days intervals to evaluate the affinities & the osteogenic activities of the human alveolar osteoblast like cells in the 3 different complexes. Result and discussion: The results indicated that the cultured human alveolar bone origin cells from 3 patients could successfully express the osteoblasts phenotype in single layered culturing in vitro after stimulated by β glycerophosphate and Dexamethasone. It has been shown that the cultured osteoblast like cells seeded on PGAS matrix had the highest attachmental, proliferative and osteogenic activities, suggesting a good bio affinity between the human alveolar osteoblast like cells and the PGAS matrix. The statistical analysis (ANOVA) showed that there were significant differences between PGAS osteoblasts complex and CLGS or LACT complexes on osteogenic activities. (P<0.05). It was also noticed that cultured human alveolar osteoblasts seeded in biodegradable materials had a delayed peak period on cell proliferation and PLAase production ,suggesting the osteoblasts seeded on scaffolds need a period of time to adjust themselves before they can normally proliferate and expres their phenotypes. Conclusion: PGAS osteoblasts complex is worth to be further developed into a tissue engineered cellular artificial bony implant for reconstructing the oral maxillofacial bony defects in a more effective way in the future. | WT4”HZ] YAO Hui | 2000 | Chinese Journal of Biomedical Engineering(English Edition)2000，9，3： | 1 |
| 7 | N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that medi- ates cell migration显示文摘 | Nishi E Prat A Hospital V | 2001 | EMBO J2001，20，13： | 1 |
| 8 | Use of pulsed light to increase the safety of ready-to-eat cured meat products显示文摘 | Ganan M Hierro E Hospital X F | 2013 | Food Control2013，32，2： | 1 |
| 9 | M N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that mediates cell migration显示文摘 | Nishi E Prat A Hospital V | 2001 | EMBO J2001，20，： | 1 |
| 10 | Use of pulsed light to increase the safety of ready-to-eat cured meat products显示文摘 | GANAN M HIERRO E HOSPITAL X F | 2013 | Food Control2013，32，： | 1 |
| 11 | N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that mediates cell migration 显示文摘 | Nishi E Prat A Hospital V Elenius K Klagsbrtm M | 2001 | EMBO J2001，20，13： | 1 |
| 12 | Italian consensus guidelines for the diagnostic work - up and follow - up of cystic pancreatic neoplasms显示文摘 | Italian Association of Hospital Gastroenterologists and Endoscopists Italian Association for the Study of the Pancreas BUSCARINI E | 2014 | Dig Liver Dis2014，46，6： | 1 |
| 13 | Studies of Permeabilities of Metallic Ions Through Crosslinked Chitosan Membranes显示文摘IntroductionChitinandchitosanrepresentthesecondlargestclassofnaturalbiopolymers,whichhavebeenreportedtobepromisingpolymersnot... | FANG Yue e , LU Xiao bing, WANG Huan ting and CHENG Qing (Department of Applied Chemistry, University of Science and Technology of China, Hefei, 230026) CHEN Bo jun, CHEN Guo jun, WANG Yong jie and WANG Shao gen (First Affiliated Hospital | 1998 | Chemical Research in Chinese Universities1998，14，4： | 0 |
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