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| 1 | Protective effect of thodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury显示文摘Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons method.After establishing the model,the rats were randomly divided into five groups.Rats in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μL).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm.Rats in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with PBS.Rats in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/mL in a 50-μL cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs.Rats in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside.One week after treatment,exercise recovery was evaluated with a modified combined behavioral score scale.Hematoxylin-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue.Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord.Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord.The results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(P<0.05),the most recovered tissue,and the greatest number of neurons,as indicated by Pischingert’s methylene blue staining.(2)Compared with the PBS group,HIF-1 protein expression was greater in the rhodioloside group(P<0.05).(3)Compared with the Ad-HIF-MSC group,Sry mRNA levels were higher in the rhodioloside+Ad-HIF-MSC group(P<0.05).These results confirm that rhodioloside combined with bone marrow mesenchymal stem cells can promote the recovery of spinal cord injury and activate the HIF-1 pathway to promote the survival of bone marrow mesenchymal stem cells and repair damaged neurons within spinal cord tissue.This experiment was approved by the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine,China(approval No.2015KYLL029)in June 2015. | Xiao-Qin Ha Bo Yang Huai-Jing Hou Xiao-Ling Cai Wan-Yuan Xiong Xu-Pan Wei | 2020 | Neural Regeneration Research2020，15，4： | 9 |
| 2 | Composite polymer networks of polyacrylamide and modified amino resin-polyurethane for oil field water shutoff显示文摘SINCE water emitting in oil wells is a general problem in developing oil fields, the workers inthe world put quite an emphasis on water shutoff. In recent years, the study on develop-ing a novel and highly effective water plugging agent has become an important technical mea-sure for controlling water emitting in the middle and later periods of an oil field. A highly ef-fective water plugging agent can form gel, gradually swell in water and shut off water, but oilcan get through. It also has suitable resistance to pressure and high temperature and a longservice life. Only when the water shutoff agent can form a composite polymer network (CPN) | Liu, QP Hou, SJ Ha, RH Yao, KD | 1997 | Chinese Science Bulletin1997，42，15： | 3 |
| 3 | Transthyretin oligomers induce calcium influx via voltage-gated calcium channels显示文摘 | Hou X Parkington HC Coleman HA | 2007 | J Neurochem2007，100，2： | 1 |
| 4 | Evolution and the clinical implica- tion mutations in acute myeloid leukemia-stability during disease DNMT3 A 显示文摘 | Hou HA Kuo YY Liu CY | 2012 | Blood2012，119，2： | 1 |
| 5 | DNMT3A mutations in acute myeloid leukemia:stability during disease evolution and clinical implications显示文摘 | Hou HA Kuo YY Liu CY | 2012 | Blood2012，119，2： | 1 |
| 6 | Clinical implications of the SETBP1 mutation in patients with primary myelodysplastic syndrome and its stability during disease progression 显示文摘 | Hou HA Kuo YY Tang JL | 2014 | Am J Hematnl2014，89，2： | 1 |
| 7 | DNMT3A mutations in acute myeloid leukemia:stability during disease evolution and clinical implications显示文摘 | Hou HA Kuo YY Liu CY | 2012 | Blood2012，119，2： | 1 |
| 8 | Effect of staurosporine on cycle of human gastric cancer cells 显示文摘 | Ha MW Hou KZ Liu YP | 2004 | World J Gastroenterol2004，10，2： | 1 |
| 9 | Distinct association between aberrant methylation of Wnt inhibitors and genetic alterations in acute myeloid leukaemia显示文摘 | Hou HA Kuo YY Liu CY | 2011 | Br J Cancer2011，105，12： | 1 |
| 10 | SF3B1 mutations in patients with myelodysplastic syndromes: the mutatio- nis stable during disease evolution显示文摘 | Lin CC Hou HA Chou WC | 2014 | Am J Hematol2014，89，8： | 1 |
| 11 | Distinct clinical and biological features of de novo acute myeloid leukemia with additional sex comb-like 1(ASXL1)mutations显示文摘 | Chou WC Huang HH Hou HA | 2010 | Blood2010，116，： | 1 |
| 12 | The clinical implica- tion of SRSF2 mutation in patients with myelodysplas- tic syndrome and its stability during disease evolution 显示文摘 | Wu S J Kuo YY Hou HA | 2012 | Blood2012，120，15： | 1 |
| 13 | WT1 mutation in470adult patients with acute myeloid leukemia:stability during disease evolution and implication of its incorporation into a survival scoring system显示文摘 | Hou HA Huang TC Lin LI | 2010 | Blood2010，115，： | 1 |
| 14 | Bone marrow angiogenesis mag-netic resonance imaging in patients with acute myeloid leukemia: peak enhancement ratio is an independent predictor for overall survival显示文摘 | Shih TF Hou HA Liu CV | 2009 | Blood2009，113，： | 1 |
| 15 | Dispersion and co-dispersion of ZrBand SiC nanopowders in ethanol显示文摘 | Zhang X Hou Y Ha P | 2012 | Ceram Int2012，38，4： | 1 |
| 16 | WT1mutation in 470adult patients with acute myeloid leukemia:stability duringdisease evolution and implication of its incorporation into asurvival scoring system显示文摘 | Hou HA Huang TC Lin LI | 2010 | Blood2010，115，25： | 1 |
| 17 | The clinical implication of SRSF2 mutation in patients with myelodysplastic syn- drome and its stability during disease evolution 显示文摘 | Wu S J Kuo YY Hou HA | 2012 | Blood2012，120，15： | 1 |
| 18 | AML1/RUNX1 mutations in 470 adult patients with de novo acute myeloid leukemia:Prognostic implication and interaction with other gene alterations显示文摘 | Tang JL Hou HA Chen CY | 2009 | Blood2009，114，： | 1 |
| 19 | Poor outcome in post transplant lymphoproliferative disorder with pulmonary in- volvement after allogeneic hematopoietic SCT: 13 years' experience in a single institute显示文摘 | Hou HA Yao M Tang JL | 2009 | Bone Marrow Trans- plant2009，43，4： | 1 |
| 20 | CEBPA methylation as a prognostic biomarker in patients with de novo acute myeloid leukemia显示文摘 | Lin TC Hou HA Chou WC | | 0，，： | 1 |
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