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| 1 | Regulation of Arabidopsis Early Anther Development by the Mitogen-Activated Protein Kinases, MPK3 and MPK6, and the ERECTA and Related Receptor-Like Kinases显示文摘激活 Mitogen 的蛋白质 kinase (MAPK ) 和表明小径的像受体的 kinase (LRR-RLK ) 被显示出调整植物生长和开发的多样的方面的充满白氨酸的重复。在 Arabidopsis,合适的花药开发依靠细胞间的通讯协调房间增长和区别。二密切相关的基因编码 MAPK, MPK3 和 MPK6,在调整有气孔的 patterning 冗余地工作。尽管 mpk6 异种减少了富饶,在花药开发的 MPK3 和 MPK6 的函数没被描绘。同样, ERECTA (嗯) , ERECTA-LIKE1 (ERL1 ) 和 ERL2 基因编码 LRR-RLKs 一起工作指导有气孔的房间命运说明和 er-105 erl1-2 erl2-1 三元组异种是无菌的。因为 mpk3 mpk6 两倍空异种是胚胎致命,花药开发在可行 mpk3/+mpk6/ 和 er-105 erl1-2 erl2-1 异种被描绘。我们发现两个变异的花药通常没能形成四片花药脑叶中的至少一个,与展出 mpk3/+mpk6/ 异种的比那些的更严重的显型的 er-105 erl1-2 erl2-1 三元组异种。区分的变异的脑叶的体的房间层显得更大,更多比的瓦解了野类型。另外, er-105 erl1-2 erl2-1 三元组异种有雄蕊,其多数拥有完全无差别或下面区分的花药的一个减少的数字。有时,而且, mpk3/+mpk6/ 异种花药不张口,并且 er-105 erl1-2 erl2-1 花药没被观察张口。因此,我们的结果显示 ER/ERL1/ERL2 和 MPK3/MPK6 在正常花药脑叶形成和花药房间区别起重要作用。在在另外的发展过程的这些基因和异种的二种类型的发展显型报导了的花药的类似之间的近功能的关系进一步这里建议这些基因可能也在一样的小径工作调整花药房间分割和区别的可能性。 | Carey L.H. Hord Yu-Jin Sun Lynn J. Pillitteri Keiko U. Torii Huachun Wang Shuqun Zhang Hong Ma | 2008 | Molecular Plant2008，1，4： | 29 |
| 2 | Residual lifetime prediction model of nonlinear accelerated degradation data with measurement error显示文摘For the product degradation process with random effect(RE), measurement error(ME) and nonlinearity in step-stress accelerated degradation test(SSADT), the nonlinear Wiener based degradation model with RE and ME is built. An analytical approximation to the probability density function(PDF) of the product's lifetime is derived in a closed form. The process and data of SSADT are analyzed to obtain the relation model of the observed data under each accelerated stress. The likelihood function for the population-based observed data is constructed. The populationbased model parameters and its random coefficient prior values are estimated. According to the newly observed data of the target product in SSADT, an analytical approximation to the PDF of its residual lifetime(RL) is derived in accordance with its individual degradation characteristics. The parameter updating method based on Bayesian inference is applied to obtain the posterior value of random coefficient of the RL model. A numerical example by simulation is analyzed to verify the accuracy and advantage of the proposed model. | Zhongyi Cai Yunxiang Chen Qiang Zhang Huachun Xiang | 2017 | Journal of Systems Engineering and Electronics2017，28，5： | 12 |
| 3 | Horizontal-Based Orchestration for Multi-Domain SFC in SDN/NFV-Enabled Satellite/Terrestrial Networks显示文摘It's promising to use Software-Defined Networking(SDN) and Network Functions Virtualization(NFV) to integrate satellite and terrestrial networks. To construct network service function chains in such a multi-domain environment, we propose a horizontal-based Multi-domain Service Function Chaining(Md-SFC) orchestration framework. In this framework, multi-domain orchestrators can coordinate with each other to guarantee the end-to-end service quality. Intra-domain orchestrators also coordinate SDN controllers and NFV management components to implement intra-domain service function chains. Based on this, we further propose a heuristic SFC mapping algorithm with a cooperative inter-domain path calculation method to map service function chains to infrastructures. In this method, master multi-domain orchestrator and intra-domain orchestrators coordinate to select proper inter-domain links. We compare the cooperative method with a naive uncooperative way that domains' topology information is provided to the master multi-domain orchestrator and it calculates the shortest inter-domain path between intra-domain service function chains directly. Simulation results demonstrate that our solution is feasible. It is able to construct end-to-end performance guaranteed service function chain by horizontal-based cooperation. The cooperative inter-domain path calculation method decreasesthe mapping load for the master orchestrator and gets the same end-to-end performance. | Guanglei Li Huachun Zhou Bohao Feng Guanwen Li Qi Xu | 2018 | China Communications2018，15，5： | 11 |
| 4 | Comparison of the effects of aluminum and iron(Ⅲ)salts on ultrafiltration membrane biofouling in drinking water treatment显示文摘Coagulation plays an important role in alleviating membrane fouling, and a noticeable problem is the development of microorganisms after long-time operation, which gradually secrete extracellular polymeric substances(EPS). To date, few studies have paid attention to the behavior of microorganisms in drinking water treatment with ultrafiltration(UF)membranes. Herein, the membrane biofouling was investigated with different aluminum and iron salts. We found that Al_2(SO_4)_3·18 H_2O performed better in reducing membrane fouling due to the slower growth rate of microorganisms. In comparison to Al_2(SO_4)_3·18H_2O,more EPS were induced with Fe_2(SO_4)_3·x18H_2O, both in the membrane tank and the sludge on the cake layer. We also found that bacteria were the major microorganisms, of which the concentration was much higher than those of fungi and archaea. Further analyses showed that Proteobacteria was dominant in bacterial communities, which caused severe membrane fouling by forming a biofilm, especially for Fe_2(SO_4)_3·x18H_2O. Additionally, the abundances of Bacteroidetes and Verrucomicrobia were relatively higher in the presence of Al_2(SO_4)_3·18 H_2O,resulting in less severe biofouling by effectively degrading the protein and polysaccharide in EPS. As a result, in terms of microorganism behaviors, Al-based salts should be given preference as coagulants during actual operations. | Xing Wang Baiwen Ma Yaohui Bai Huachun Lan Huijuan Liu Jiuhui Qu | 2018 | Journal of Environmental Sciences2018，30，1： | 8 |
| 5 | Environmental changes during modern period from the record of Gaoyou Lake sediments, Jiangsu显示文摘基于从高伊奥·莱克拿的核心的参数的分析,包括磁性的危险性,谷物尺寸特征和沉积的率,环境变化与历史的记录和高伊奥·莱克水水平材料的帮助在现代时期期间被检验。在现代时期期间,磁性的危险性和一种更低的沉积谷物尺寸的更高的价值与湿气候与一致,这被结束,当磁性的危险性和一种更高的谷物尺寸的更低的价值与干燥气候是相关的时。结果显示在到 2003AD 的从 1880 的 123 年时期的气候能被划分成四个阶段:二个低水水平阶段(1880 鈥 ? 915AD, 1948 鈥 ? 981AD ) 并且二个高水位线水平阶段(1915 鈥 ? 948AD, 1981 鈥 ? 003AD ) 。看来,地区性的气候通常在 30 年的周期经历了一个 dry-wet-dry-wet 模式。目前,它在一个湿时期的结束,因此地区性的气候被期望在不久的将来变得干燥。这个结论在 Gaoyou 区域的历史的文学与气候记录相应,并且它也在诺思江苏区域与气候的变化匹配。 | LI Shuheng FU Guanghe GUO Wei HE Huachun ZHANG Zhenke | 2007 | Journal of Geographical Sciences2007，17，1： | 5 |
| 6 | Remaining useful lifetime prediction for equipment based on nonlinear implicit degradation modeling显示文摘Nonlinearity and implicitness are common degradation features of the stochastic degradation equipment for prognostics.These features have an uncertain effect on the remaining useful life(RUL)prediction of the equipment.The current data-driven RUL prediction method has not systematically studied the nonlinear hidden degradation modeling and the RUL distribution function.This paper uses the nonlinear Wiener process to build a dual nonlinear implicit degradation model.Based on the historical measured data of similar equipment,the maximum likelihood estimation algorithm is used to estimate the fixed coefficients and the prior distribution of a random coefficient.Using the on-site measured data of the target equipment,the posterior distribution of a random coefficient and actual degradation state are step-by-step updated based on Bayesian inference and the extended Kalman filtering algorithm.The analytical form of the RUL distribution function is derived based on the first hitting time distribution.Combined with the two case studies,the proposed method is verified to have certain advantages over the existing methods in the accuracy of prediction. | CAI Zhongyi WANG Zezhou CHEN Yunxiang GUO Jiansheng XIANG Huachun | 2020 | Journal of Systems Engineering and Electronics2020，31，1： | 5 |
| 7 | Characteristic model based all-coefficient adaptive control of an AMB suspended energy storage flywheel test rig显示文摘Feedback control of active magnetic bearing(AMB) suspended energy storage flywheel systems is critical in the operation of the systems and has been well studied. Both the classical proportional-integralderivative(PID) control design method and modern control theory, such as H∞ control and μ-synthesis,have been explored. PID control is easy to implement but is not effective in handling complex rotordynamics. Modern control design methods usually require a plant model and an accurate characterization of the uncertainties. In each case, few experimental validation results on the closed-loop performance are available because of the costs and the technical challenges associated with the construction of experimental test rigs.In this paper, we apply the characteristic model based all-coefficient adaptive control(ACAC) design method for the stabilization of an AMB suspended flywheel test rig we recently constructed. Both simulation and experimental results demonstrate strong closed-loop performance in spite of the simplicity of the control design and implementation. | Xujun LYU Long DI Zongli LIN Yefa HU Huachun WU | 2018 | Science China(Information Sciences)2018，61，11： | 4 |
| 8 | Remaining lifetime prediction for nonlinear degradation device with random effect显示文摘For the large number of nonlinear degradation devices existing in a project, the existing methods have not systematically studied the effects of random effect on the remaining lifetime(RL),the accuracy and efficiency of the parameters estimation are not high, and the current degradation state of the target device is not accurately estimated. In this paper, a nonlinear Wiener degradation model with random effect is proposed and the corresponding probability density function(PDF) of the first hitting time(FHT)is deduced. A parameter estimation method based on modified expectation maximum(EM) algorithm is proposed to obtain the estimated value of fixed coefficient and the priori value of random coefficient in the model. The posterior value of the random coefficient and the current degradation state of target device are updated synchronously by the state space model(SSM) and the Kalman filter algorithm. The PDF of RL with random effect is deduced. A simulation example is analyzed to verify that the proposed method has the obvious advantage over the existing methods in parameter estimation error and RL prediction accuracy. | CAI Zhongyi CHEN Yunxiang GUO Jiansheng ZHANG Qiang XIANG Huachun | 2018 | Journal of Systems Engineering and Electronics2018，29，5： | 4 |
| 9 | How Shenzhen, China avoided widespread community transmission: a potential model for successful prevention and control of COVID-19显示文摘Shenzhen is a city of 22 million people in south China that serves as a financial and trade center for East Asia.The city has extensive ties to Hubei Province,the first reported epicenter of the coronavirus disease 2019(COVID-19)outbreak in the world.Initial predictions suggested Shenzhen would experience a high number of COVID-19 cases.These predictions have not materialized.As of 31 March 2020 Shenzhen had only 451 confirmed cases of COVID-19.Contact tracing has shown that no cases were the result of community transmission within the city.While Shenzhen did not implement a citywide lockdown like Wuhan,it did put into place a rapid response system first developed after the severe acute respiratory syndrome(SARS)epidemic in 2003.In the wake of the 2003 SARS outbreak,Shenzhen health authority created a network for surveillance and responding to novel respiratory infections,including pneumonia of unknown causes(PUC).The network rapidly detected mass discussion about PUC and immediately deployed emergency preparedness,quarantine for close contacts of PUC.Five early actions(early detection,early reporting,early diagnosis,early isolation,and early treatment)and four centralized responses(centralized coordination by experts,centralized allocation of resources,centralized placement of patients,and centralized provision of treatment)ensured effective prevention and control.Tripartite working teams comprising community cadres,medical personnel and police were formulated to conduct contact tracing at each neighborhood and residential community.Incorporation of mobile technology,big data,and artificial intelligence into COVID-19 response increased accessibility to health services,reduced misinformation and minimized the impact of fake news.Shenzhen’s unique experience in successfully controlling the COVID-19 outbreak may be a useful model for countries and regions currently experiencing rapid spread of the virus. | Huachun Zou Yuelong Shu Tiejian Feng | 2020 | Infectious Diseases of Poverty2020，9，4： | 4 |
| 10 | Comparing the adsorption behaviors of Cd, Cu and Pb from water onto Fe-Mn binary oxide, MnO2 and FeOOH显示文摘FMBO 的吸附潜力, FeOOH , MnO <潜水艇class=“ a-plus-plus ”>为 Cd 的移动的 2 <啜class=“ a-plus-plus ”>2+, Cu <啜class=“ a-plus-plus ”>2+和 Pb <啜class=“ a-plus-plus ”>在水的系统的2+在这研究被调查。比作 FMBO 和 FeOOH, MnO < 潜水艇 class= “ a-plus-plus ” > 2 向三个金属离子为高得多的移动提供了能力。 MnO 的最大的吸附能力<潜水艇class=“ a-plus-plus ”>为 Cd 的 2 <啜class=“ a-plus-plus ”>2+, Cu <啜class=“ a-plus-plus ”>2+和 Pb <啜class=“ a-plus-plus ”>2+是 1.23 , 2.25 和 2.60 mmol ???????剕偅??????????????????????￡?? 蓈 ?? | Wei XU Huachun LAN Hongjie WANG Hongming LIU Jiuhui QU | 2015 | Frontiers of Environmental Science & Engineering2015，9，3： | 4 |
| 11 | Modeling uniaxial tensile deformation of polycrystalline Al using CPFEM显示文摘The crystal plasticity finite element modeling(CPFEM)is realized in commercial finite element code ABAQUS with UMAT subroutine on the basis of the crystal plasticity theory of rate dependent polycrystal constitutive relations in the mesoscopic scale.The initial orientations obtained by electron backscatter diffraction(EBSD)are directly input into the CPFEM to simulate the mechanical response of polycrystaUine 1050 pure Al in uniaxial tensile deformation.Two polycrystal models and two tensile strain rates were used in the simulations.The stress-strain curves of tensile deformation were analyzed.The predictions and the corre- sponding experiment result show reasonable agreement and slight deviation with experiments.The flow true stress of strain rate 0.01 s^(-1)is higher than that of strain rate 0.001 s^(-1).At the strain less than 0.05,the stress saturated rate of the experiment is higher than the simulated results.However,the stress saturated rate of the experiment becomes gentler than the corresponding simulated predictions at the strain over 0.05.Also,necking was simulated by the two models,but the necking strain is not well predicted.Tensile textures at strain 0.25 were predicted at the low strain rate of 0.001 s^(-1).The predictions are in good accord with the experimental results. | Huachun pi Jingtao Han Chuanguo Zhang A. Kiet Tieu Zhengyi Jiang | 2008 | Journal of University of Science and Technology Beijing2008，15，1： | 4 |
| 12 | Identification of a Differentially-expressed Gene in Fatty Liver of Overfeeding Geese显示文摘响应 overfeeding,鹅得脂肪肝。理解肥育的机制, mRNA 微分显示反转录 PCR 被用来处于 overfeeding 并且正常的喂的条件学习法语的 Landes 灰鹅和 Xupu 白鹅之间的基因表达差别。一基因被发现在在两个品种的脂肪肝起来调整,并且它与83%身份有 1797 bp cDNA 到揭示的鸡 SELENBP1.The 顺序分析它 1413 bp 的开的读物框架编码 471 氨基酸的蛋白质,它与高相同包含 56 kDa 硒绑定蛋白质的通常认为的保存的域到它鸡肉(95%)的相当或相同事物,老鼠(86%),老鼠(84%),人(86%),猴子(86%),狗(86%),并且这蛋白质的牛(86%) .Thefunction 简短基于出版信息被考察了。在织物表达式分析,鹅 SELENBP1 mRNA 的表达式被发现比在另外的测试纸巾在肝或肾更高。结果证明 overfeeding 能增加鹅 SELENBP1 的 mRNA 表达式水平。 | Ayong ZHAO Huachun TANG Sufang LU Ruiguo HE | 2007 | Acta Biochimica et Biophysica Sinica2007，39，9： | 4 |
| 13 | The effects of hydrogen peroxide pre-oxidation on ultrafiltration membrane biofouling alleviation in drinking water treatment显示文摘Pre-oxidation is widely used to reduce ultrafiltration membrane fouling.However,the variation in the composition of microbial communities and extracellular polymeric substances(EPSs) accompanying pre-oxidation in drinking water treatment has received little attention.In this study,hydrogen peroxide(H_2O_2) was used in a coagulationultrafiltration process with Al_2(SO_4)_3·18H_2O.A long-term reactor experiment(60 d) showed that pre-oxidation alleviated membrane fouling,mainly due to its inhibition of microbial growth,as observed by flow cytometry measurements of the membrane tank water.Further analysis of the formed cake layer demonstrated that the corresponding levels of EPS released from the microbes were lower with than without H_2O_2 treatment.In comparison to polysaccharides,proteins dominated the EPS.2 D-electrophoresis showed little difference(p > 0.05,Student's t-test) in the composition of proteins in the cake layer between the treatments with and without H_2O_2.The molecular weights of proteins ranged from approximately 30–50 kDa and the majority of isoelectric points ranged from 6 to 8.Highthroughput sequencing showed that the predominant bacteria were Proteobacteria,Bacteroidetes,and Verrucomicrobia in both cake layers.However,the relative abundance of Planctomycetes was higher in the cake layer with H_2O_2 pre-oxidation,which was likely probably due to the strong oxidative resistance of its cell wall.Overall,our findings clarify the fundamental molecular mechanism in H_2O_2 pre-oxidation for ultrafiltration membrane bio-fouling alleviation in drinking water treatment. | Xing Wang Baiwen Ma Yaohui Bai Huachun Lan Huijuan Liu Jiuhui Qu | 2018 | Journal of Environmental Sciences2018，30，11： | 4 |
| 14 | HF radar sea clutter rejection by nonlinear projections显示文摘In the background of signal detection for high frequency (HF) radar, the sea clutter is quite significant and can mask some weak target signals. A new clutter rejection method named “nonlinear projection” is given to improve the SNR of the target. This approach is based on the recent observation that HF sea clutter may be modeled as a nonlinear deterministic dynamical system. After approximating the multidimensional reconstruction of the clutter by a lowdimensional attractor, projections onto this attractor can separate the clutter from other components. Real sea clutter, simulated target data and real target data are used to show that a nonlinear clutter rejection method is a promising technique to suppress sea clutter and enhances target detection. | Zhou Gongjian Jin Yonggao Dong Huachun Quan Taifan | 2005 | Journal of Systems Engineering and Electronics2005，16，4： | 3 |
| 15 | Distributed Contact Plan Design for Multi-Layer Satellite-Terrestrial Network显示文摘In multi-layer satellite-terrestrial network, Contact Graph Routing(CGR) uses the contact information among satellites to compute routes. However, due to the resource constraints in satellites, it is extravagant to configure lots of the potential contacts into contact plans. What's more, a huge contact plan makes the computing more complex, which further increases computing time. As a result, how to design an efficient contact plan becomes crucial for multi-layer satellite network, which usually has a large scaled topology. In this paper, we propose a distributed contact plan design scheme for multi-layer satellite network by dividing a large contact plan into several partial parts. Meanwhile, a duration based inter-layer contact selection algorithm is proposed to handle contacts disruption problem. The performance of the proposed design was evaluated on our Identifier/Locator split based satellite-terrestrial network testbed with 79 simulation nodes. Experiments showed that the proposed design is able to reduce the data delivery delay. | Wenfeng Shi Deyun Gao Huachun Zhou Bohao Feng Haifeng Li Guanwen Li Wei Quan | 2018 | China Communications2018，15，1： | 3 |
| 16 | LBMA: A Novel Locator Based Mobility Support Approach in Named Data Networking显示文摘To enhance seamless source mobility support in the mobile NDN(Named Data Networking) environment,we propose a novel Locator Based Mobility support Approach,dubbed LBMA.In this approach,we assign a unique topologically layered locator to each AR(Access Route)in NDN network,and extend the AR with additional functionalities,such as caching and forwarding Interest packets on behalf of the source.Meanwhile,we add an optional field to the original NDN packet,and modify the Outgoing Interface(s) field in the AR's original FIB(Forwarding Information Base)entries,to record the mobility status and the current locator of the source.Specifically,the consumer in LBMA can continue the interrupted communication,without delivering Interest packets towards the old location of the source,nor waiting for all relevant devices completing the FIB entries updating.The performance analysis result indicates that LBMA has lower handoff cost and shorter handoff latency,compared with other existing source mobility support approaches. | RAO Ying LUO Hongbin GAO Deyun ZHOU Huachun ZHANG Hongke | 2014 | China Communications2014，11，4： | 3 |
| 17 | Application of Neural Network in Fault Location of Optical Transport Network显示文摘Due to the increasing variety of information and services carried by optical networks, the survivability of network becomes an important problem in current research. The fault location of OTN is of great significance for studying the survivability of optical networks. Firstly, a three-channel network model is established and analyzing common alarm data, the fault monitoring points and common fault points are carried out. The artificial neural network is introduced into the fault location field of OTN and it is used to judge whether the possible fault point exists or not. But one of the obvious limitations of general neural networks is that they receive a fixedsize vector as input and produce a fixed-size vector as the output. Not only that, these models is even fixed for mapping operations (for example, the number of layers in the model). The difference between the recurrent neural network and general neural networks is that it can operate on the sequence. In spite of the fact that the gradient disappears and the gradient explodes still exist in the neural network, the method of gradient shearing or weight regularization is adopted to solve this problem, and choose the LSTM (long-short term memory networks) to locate the fault. The output uses the concept of membership degree of fuzzy theory to express the possible fault point with the probability from 0 to 1. Priority is given to the treatment of fault points with high probability. The concept of F-Measure is also introduced, and the positioning effect is measured by using location time, MSE and F-Measure. The experiment shows that both LSTM and BP neural network can locate the fault of optical transport network well, but the overall effect of LSTM is better. The localization time of LSTM is shorter than that of BP neural network, and the F1-score of LSTM can reach 0.961566888396156 after 45 iterations, which meets the accuracy and real-time requirements of fault location. Therefore, it has good application prospect and practical value to introduce neural network into the fault location field of optical transport network. | Tianyang Liu Haoyuan Mei Qiang Sun Huachun Zhou | 2019 | China Communications2019，16，10： | 3 |
| 18 | Functional genomic studies on an immune-and antiviral-related gene of MyD88 in orange-spotted grouper,Epinephelus coioides显示文摘Myeloid differentiation factor 88(MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB(NF-κB) activation.In this study,a new MyD88 gene(designated as Og-MyD88) was cloned from orange-spotted grouper,Epinephelus coioides,based on the expressed sequence tag(EST) obtained following Roche 454 GS-FLX sequencing.The full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca chuatsi.The deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R(TIR) domain at the carboxyl terminus,as well as three highly conserved motifs(Box1,Box2 and Box3) within the C-terminal TIR domain.In healthy fish,Og-MyD88 was found to be strongly expressed in immune-related tissues,including the spleen,head kidney,kidney,liver,skin and intestine,with lower expression in heart,stomach,brain and muscle.Transcripts of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus(SGIV),a highly lethal viral pathogen to grouper fish.Furthermore,the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-κB activity in HEK-293 cells.Overexpressed Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen(GS) cells.These results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates. | YAN Yang CUI HuaChun WEI JingGuang HUANG YouHua HUANG XiaoHong QIN QiWei | 2012 | Chinese Science Bulletin2012，57，25： | 2 |
| 19 | Effect of amino acid mutation at position 127 in 3A of a rabbitattenuated foot-and-mouth disease virus serotype Asia1 on viral replication and infection显示文摘An amino acid mutation(R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine(I) was changed to arginine(R) in the infectious cDNA clone of the rabbit-attenuated ZB strain by sitedirected mutagenesis, and the R127-mutated virus was rescued. BHK monolayer cells and suckling mice were inoculated with the R127-mutated virus to test its growth property and pathogenicity, respectively. The effects of the R127 mutation on viral replication and virulence were analyzed. The data showed that there was a slight difference in plaque morphology between the R127-mutated and wild-type viruses. The growth rate of the mutated virus was lower in BHK-21 cells and its virulence in suckling mice was also attenuated. This study indicates that the R127 mutation in 3A may play an important role in FMDV replication in vitro and in pathogenicity in suckling mice. | Aiguo Xin Mingwang Zhu Qi Hu Haisheng Miao Zhenqi Peng Yuwen He Lin Gao Huachun Li | 2014 | Virologica Sinica2014，29，5： | 2 |
| 20 | Safety of SARS-CoV-2 vaccines:a systematic review and meta-analysis of randomized controlled trials显示文摘Background Various modalities of vaccines against coronavirus disease 2019(COVID-19),based on different platforms and immunization procedures,have been successively approved for marketing worldwide.A comprehensive review for clinical trials assessing the safety of COVID-19 vaccines is urgently needed to make an accurate judgment for mass vaccination.Main text A systematic review and meta-analysis was conducted to determine the safety of COVID-19 vaccine candidates in randomized controlled trials(RCTs).Data search was performed in PubMed,Embase,Cochrane library,Scopus,Web of Science,and MedRxiv.Included articles were limited to RCTs on COVID-19 vaccines.A total of 73,633 subjects from 14 articles were included to compare the risks of adverse events following immunization(AEFI)after vaccinating different COVID-19 vaccines.Pooled risk ratios(RR)of total AEFI for inactivated vaccine,viral-vectored vaccine,and mRNA vaccine were 1.34[95%confidence interval(CI)1.11–1.61,P<0.001],1.65(95%CI 1.31–2.07,P<0.001),and 2.01(95%CI 1.78–2.26,P<0.001),respectively.No significant differences on local and systemic AEFI were found between the first dose and second dose.In addition,people aged≤55 years were at significantly higher risk of AEFI than people aged≥56 years,with a pooled RR of 1.25(95%CI 1.15–1.35,P<0.001).Conclusions The safety and tolerance of current COVID-19 vaccine candidates are acceptable for mass vaccination,with inactivated COVID-19 vaccines candidates having the lowest reported AEFI.Long-term surveillance of vaccine safety is required,especially among elderly people with underlying medical conditions. | Musha Chen Yue Yuan Yiguo Zhou Zhaomin Deng Jin Zhao Fengling Feng Huachun Zou Caijun Sun | 2021 | Infectious Diseases of Poverty2021，10，4： | 2 |
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