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1篇 您的检索式：作者名="Huahang Yu"

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		题名	作者	年代	出处	被引量
	1	A microfluidic surface-enhanced Raman scattering(SERS) sensor for microRNA in extracellular vesicles with nucleic acid-tyramine cascade amplification显示文摘Exosomal micro RNA(mi RNA) is an ideal candidate of noninvasive biomarker for the early diagnosis of cancer. Sensitive and accurate sensing of abnormal exosomal mi RNA plays essential role for clinical promotion due to its close correlation with tumor proliferation and progression. Herein, a microfluidic surface-enhanced Raman scattering(SERS) sensor was proposed for an on-line detection of exosomal mi RNA based on rolling circle amplification(RCA) and tyramine signal amplification(TSA) strategy. The microfluidic chip consists of a magnetic enrichment chamber, a serpentine fluidic mixer and a plasmonic SERS substrate functionalized with capture probes. The released mi RNA activates the capture probe, triggers RCA reaction, and generates a large number of single-stranded DNA products to drive the catalysis of nanotags deposition via TSA, producing numerous “hot spots” to enhance the SERS signals. In merit of the microfluidics chip and nucleic acid-tyramine cascade amplification, the developed SERS sensor significantly improves the sensitivity for the exosomal mi RNA assay, resulting in a limit of detection(LOD) as low as 1 pmol/L and can be successfully applied in the analysis of exosomes secreted from breast tumor cells, which demonstrates the potential utility in practical applications.	Yue Zhao Xiaoxing Fang Min Bai Jin Zhang Huahang Yu Feng Chen Yongxi Zhao	2022	Chinese Chemical Letters2022，33，4：	0

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