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| 1 | Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers显示文摘 | Chen Wu Yuanyuan Xie Feng Gao Yanan Wang Yawei Guo Huanna Tian Yanshuang Li Wufang Fan | 2013 | Gene2013，，1： | 2 |
| 2 | GALNT14 mediates tumor invasion and migration in breast cancer cell MCF-7显示文摘 | Huanna T Tao Z Xiangfei W | | Mol Carcinog0，54，10： | 1 |
| 3 | New xylose transporters support the simultaneous consumption of glucose and xylose in Escherichia coli显示文摘Glucose and xylose are two major components of lignocellulose.Simultaneous consumption of glucose and xylose is critical for engineered microorganisms to produce fuels and chemicals from lignocellulosic biomass.Although many production limitations have been resolved,glucose‐induced inhibition of xylose transport remains a challenge.In this study,a cell growthbased screening strategy was designed to identify xylose transporters uninhibited by glucose.The glucose pathway was genetically blocked in Escherichia coli so that glucose functions only as an inhibitor and cells need xylose as the carbon source for survival.Through adaptive evolution,omics analysis and reverse metabolic engineering,a new phosphoenolpyruvate:carbohydrate phosphotransferase system(PTS)galactitol transporter(GalABC,encoded by EcolC_1640,EcolC_1641,and EcolC_1642 genes)that is not inhibited by glucose was identified.Inactivation of adenylate cyclase led to increased expression of the EcolC_1642 gene,and a point mutation in gene EcolC_1642(N13S)further enhanced xylose transport.During the second round of gene mining,AraE and a new ABC transporter(AraFGH)of xylose were identified.A point mutation in the transcription regulator araC(L156I)caused increased expression of araE and araFGH genes without arabinose induction,and a point mutation in araE(D223Y)further enhanced xylose transport.These newly identified xylose transporters can support the simultaneous consumption of glucose and xylose and have potential use in producing chemicals from lignocellulose. | Xinna Zhu Feiyu Fan Huanna Qiu Mengyao Shao Di Li Yong Yu Changhao Bi Xueli Zhang | 2022 | mLife2022，1，2： | 0 |
| 4 | Study on the Expression of Dopamine Receptor-3(DRD3) in Rats after Sacral Spinal Cord Transection显示文摘[Objectives] This study aimed to study the distribution characteristics of DRD3(dopamine receptor-3) and the changes in its expression before and after spinal cord injury(SCI), in order to lay a morphological basis for later research. [Methods] Adult male Wistar rats were randomly divided into sham operation group and SCI group. The rat spinal cord transection model at the sacral 2(S_2) segment was established. Rat tail spasticity score was performed 60 d after SCI, and the rats with 4-5 points were screened for perfusion. The expression of DRD3 in the sacral spinal cord(S+C segment) was detected by immunofluorescence. [Results] In normal rats, DRD3 was mainly distributed in the dorsal horn(DH), intermediate zone(IMZ) and ventral horn(VH) of the gray matter. It was also expressed in the white matter of the spinal cord. After SCI, the distribution of DRD3 in the segment below the injury section was similar to that of normal rats. However, the expression was different(P<0.05). [Conclusions] There was no significant change in the distribution of DRD3 in spinal cord after SCI. After the spinal cord S_2 was completed transected, the expression of DRD3 was significantly reduced in the DH, IMZ and VH regions of the gray matter of the spinal cord. | Ying CHEN Xiang ZHANG Meng CHEN Xiaoguang WU Huanna TIAN Shufang MAO Xin MENG Liqun REN | 2019 | Medicinal Plant2019，10，1： | 0 |
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