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| 1 | Functional recovery and microenvironmental alterations in a rat model of spinal cord injury following human umbilical cord blood-derived mesenchymal stem cells transplantation显示文摘BACKGROUND: Transplantation of human umbilical cord blood-derived mesenchymal stem cells (MSCs) has been shown to benefit spinal cord injury (SCI) repair. However, mechanisms of microenvironmental regulation during differentiation of transplanted MSCs remain poorly understood. OBJECTIVE: To observe changes in nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and interleukin-8 (IL-8) expression following transplantation of human umbilical cord-derived MSCs, and to explore the association between microenvironment and neural functional recovery following MSCs transplantation. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Department of Orthopedics, First Affiliated Hospital of Soochow University from April 2005 to March 2007. MATERIALS: Human cord blood samples were provided by the Department of Gynecology and Obstetrics, First Affiliated Hospital of Soochow University. Written informed consent was obtained. METHODS: A total of 62 Wister rats were randomly assigned to control (n = 18), model (n = 22, SCI + PBS), and transplantation (n = 22, SCI + MSCs) groups. The rat SCI model was established using the weight compression method. MSCs were isolated from human umbilical cord blood and cultured in vitro for several passages. 5-bromodeoxyuridine (BrdU)-labeled MSCs (24 hours before injection) were intravascularly transplanted. MAIN OUTCOME MEASURES: The rats were evaluated using the Basso, Beattie and Bresnahan (BBB) locomotor score and inclined plane tests. Transplanted cells were analyzed following immunohistochemistry. Enzyme-linked immunosorbant assay was performed to determine NGF, BDNF, and IL-8 levels prior to and after cell transplantation. RESULTS: A large number of BrdU-positive MSCs were observed in the SCI region of the transplantation group, and MSCs were evenly distributed in injured spinal cord tissue 1 week after transplantation. BBB score and inclined plane test results revealed significant functional improvement in the transplantation group compared to the model group (P < 0.05), which was maintained for 2-3 weeks. Compared to the model group, NGF and BDNF levels were significantly increased in the injured region following MSCs transplantation at 3 weeks (P < 0.05), but IL-8 levels remained unchanged (P > 0.05). CONCLUSION: MSCs transplantation increased NGF and BDNF expression in injured spinal cord tissue. MSCs could promote neurological function recovery in SCI rats by upregulating NGF expression and improving regional microenvironments. | Hongtao Zhang Huilin Yang Huanxiang Zhang Jing Qu | 2010 | Neural Regeneration Research2010，5，3： | 3 |
| 2 | Roles of lipoprotein receptors in the entry of hepatitis C virus显示文摘Infection by hepatitis C virus(HCV), a plus-stranded RNA virus that can cause cirrhosis and hepatocellular carcinoma, is one of the major health problems in the world. HCV infection is considered as a multistep complex process and correlated with abnormal metabolism of lipoprotein. In addition, virus attacks hepatocytes by the initial attaching viral envelop glycoprotein E1/E2 to receptors of lipoproteins on host cells. With the development of HCV model system, mechanisms of HCV cell entry through lipoprotein uptake and its receptor have been extensively studied in detail. Here we summarize recent knowledge about the role of lipoprotein receptors, scavenger receptor class B type Ⅰ and low-density lipoprotein receptor in the entry of HCV, providing a foundation of novel targeting therapeutic tools against HCV infection. | Jingya Lyu Hitomi Imachi Kensaku Fukunaga Takuo Yoshimoto Huanxiang Zhang Koji Murao | 2015 | World Journal of Hepatology2015，7，24： | 3 |
| 3 | Differential Expressing of Centromere Protein CenpG in Breast Cancer显示文摘Using indirect immunofluorescence (IIF), an anti-centromere protein CenpG-serum was verified. Western blot of the protein extracts of 31 samples of breast cancer tissues and their normal (not cancerous ) tissues a little far away from them in the same individuals showed that, in the majority of the tests (71%), centromere protein CenpG over expressed in breast cancer tissues. And moreover, a kind of protein component whose molecular weight is 43 kd, and which can be recognized by anti-CenpG serum was found in two of the cancer samples. The results suggested that CenpG (together whith it, there may be other relative components),which has been found and named recently, may be related to cancer,and its differential expressing is probably related to malignant cell proliferation. | 梁前进 Lu Xiangfeng Cheng Xiaolei Zhang Huanxiang He Dacheng WANG Yongchao | 2002 | High Technology Letters2002，8，1： | 3 |
| 4 | Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation显示文摘In this study, Schwann cells, at a density of 1 × 105 cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. Schwann cells cultured on the silk fibroin nanofibers appeared more ordered, their processes extended further, and they formed more extensive and complex interconnections. In addition, the silk fibroin nanofibers had no impact on the proliferation of Schwann cells or on the secretion of ciliary neurotrophic factor, brain-derived neurotrophic factor or nerve growth factor. These findings indicate that regenerated electrospun silk fibroin nanofibers can promote Schwann cell adhesion, growth and proliferation, and have excellent biocompatibility. | Aijun Hu Baoqi Zuo Feng Zhang Qing Lan Huanxiang Zhang | 2012 | Neural Regeneration Research2012，7，15： | 2 |
| 5 | QSAR and classification study of 1,4-dihydropyridine calcium channel an- tagonists based on least squares support vector machines 显示文摘 | Yao Xiaojun Liu Huanxiang Zhang Ruisheng | 2005 | Mo- lecular Pharmaceutics2005，2，5： | 1 |
| 6 | Upregulation of stromal cell-derived factor-1 alpha/CXCR4 axis-induced migration of human neural progenitors by tumor necrosis factor-alpha and interleukin-8显示文摘BACKGROUND:Studies of several animal models of central nervous system diseases have shown that neural progenitor cells(NPCs) can migrate to injured tissues.Stromal cell-derived factor 1 alpha (SDF-1α),and its primary physiological receptor CXCR4,have been shown to contribute to this process. OBJECTIVE:To investigate migration efficacy of human NPCs toward a SDF-1αgradient,and the regulatory roles of tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in SDF-1α/CXCR4 axis-induced migration of NPCs. DESIGN,TIME AND SETTING:An in vitro,randomized,controlled,cellular and molecular biology study was performed at the Laboratory of Department of Cell Biology,Medical College of Soochow University between October 2005 and November 2007. MATERIALS:SDF-1αand mouse anti-human CXCR4 fusion antibody were purchased from R&D Systems,USA.TNF-αwas purchased from Biomyx Technology,USA and IL-8 was kindly provided by the Biotechnology Research Institute of Soochow University. METHODS:NPCs isolated from forebrain tissue of 9 to 10-week-old human fetuses were cultured in vitro.The cells were incubated with 0,20,and 40 ng/mL TNF-α,or 0,20,and 40 ng/mL IL-8,for 48 hours prior to migration assay.For antibody-blocking experiments,cells were further pretreated with 0,20,and 40μg/mL mouse anti-human CXCR4 fusion antibody for 2 hours.Subsequently,the transwell assay and CXCR4 blockade experiments were performed to evaluate migration of human NPCs toward a SDF-1αgradient.Serum-free culture medium without SDF-1αserved as the negative control. MAIN OUTCOME MEASURES:The transwell assay was performed to evaluate migration of human NPCs toward a SDF-1αgradient,which was blocked by fusion antibody against CXCR4.In addition, CXCR4 expression in human NPCs stimulated by TNF-αand IL-8 was measured by flow cytometry. RESULTS:Results from the transwell assay demonstrated that SDF-1αwas a strong chemoattractant for human NPCs(P<0.01),and 20 ng/mL produced the highest levels of migration. Anti-human CXCR4 fusion antibody significantly blocked the chemotactic effect(P<0.05).Flow cytometry results showed that treatment with TNF-αand IL-8 resulted in increased CXCR4 expression and greater chemotaxis efficiency of NPCs towards SDF-1α(P<0.01). CONCLUSION:These results demonstrated that SDF-1αsignificantly attracted NPCs in vitro,and neutralizing anti-CXCR4 antibody could block part of this chemotactic function.TNF-αand IL-8 increased chemotaxis efficiency of NPCs towards the SDF-1αgradient by upregulating CXCR4 expression in NPCs. | Jing Qu Hongtao Zhang Guozhen Hui Xueguang Zhang Huanxiang Zhang | 2009 | Neural Regeneration Research2009，4，11： | 0 |
| 7 | Co-culture of astrocytes with neurons from injured brain A time-dependent dichotomy显示文摘As supportive cells for neuronal growth and development,much effort has been devoted to the role of astrocytes in the normal state.However,the effect of the astrocytes after injury remains elusive.In the present study,neurons isolated from the subventricular zone of injured neonatal rat brains were co-cultured with astrocytes.After 6 days,these astrocytes showed a mature neuron-like appearance and the number of surviving neurons,primary dendrites and total branches was significantly higher than those at 3 days.The neurons began to shrink at 9 days after co-culture with shorter and thinner processes and the number of primary dendrites and total branches was significantly reduced.These experimental findings indicate that astrocytes in the injured brain promote the development of neurons in the early stages of co-culture while these cells reversely inhibit neuronal growth and development at the later states. | Xiaojing Xu Min Wang Jing Liu Jingya Lv Yanan Hu Huanxiang Zhang | 2011 | Neural Regeneration Research2011，6，35： | 0 |
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