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| 1 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 2 | Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency显示文摘Pluripotency,一个细胞的能力区分并且产生所有胚胎的系,定义象胚胎的茎(ES ) 那样的哺乳动物的细胞类型的一个小数字细胞。当它通常被保持了时,那 pluripotency 是 transcriptional 的产品激活并且维持关键干细胞基因的表达式的规章的网络,积累的证据在建立并且保卫 ES 房间的 pluripotency 为 epigenetic 进程正在指向一个关键角色,象维持区分的房间类型的身份一样。以便更好在 pluripotency 理解 epigenetic 机制的角色,我们检验了在经历区别进一个 mesendodermal 系的人的 ES 房间(hESCs ) 染色体宽的染色质修正的动力学。我们发现在倡导者的染色质修正在区别期间仍然保持大部分不变,除了在在在 H3K27 的 acetylation 和 methylation 之间的一个动态开关标记在基因表示的激活和 silencing 之间的转变的倡导者的一个小数字,建议在在大多数差别上的房间命运承诺的一个层次表示了基因。我们也在 50 000 潜在的 enhancers 上印射,并且在染色质修正,特别 H3K4me1 和 H3K27ac 观察了大得多的动力学,它与他们的潜在的目标基因的表示相关。这些 enhancers 的进一步的分析揭示了 pluripotency 和可以在 hESCs 授与发展胜任的一个平衡状态的一口染色质签名陈述语气的潜在地关键的 transcriptional 管理者。我们的结果提供在定义 enhancers 和 pluripotency 支持染色质修正的角色的新证据。 | R David Hawkins Gary C Hon Chuhu Yang Jessica E Antosiewicz-Bourget Leonard K Lee Que-Minh Ngo Sarit Klugman Keith A Ching Lee E Edsall Zhen Ye Samantha Kuan Pengzhi Yu Hui Liu Xinmin Zhang Roland D Green Victor V Lobanenkov Ron Stewart James A Thomson Bing Ren | 2011 | Cell Research2011，21，10： | 6 |
| 3 | Effect of monovalent anions on cationic Gemini micro-emulsion显示文摘Micro-emulsion usually consists of water, oil, surfactants and co-surfactants, and each component has an effect on the phase behavior and solubilization of the micro-emulsion. When the surfactant in the micro-emulsion system is quaternary ammonium cationic Gemini surfactant, the surfactant mainly combines with the anions in the salt. With the increase of salt concentration, the phase transformation of Winsor I → Winsor III → Winsor II occurred, but the optimum salinity and salt width are different because of the type of salt. The effects of 5 different kinds of monovalent anions, including C_6H_5SO_3^-, I-, Br-, NO_3^- and Cl-, on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are researched by Winsor phase diagram. It is found that the effects of organic anions C_6H_5SO_3-and I-on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are most significant, and the effects of Br-, NO_3^- and Cl-are less significant. Meanwhile, when the optimum solubilization is achieved, the amount of sodium benzoate is the least, indicating that the organic anion has stronger self-organization behavior with quaternary ammonium cationic Gemini surfactants. | Yinglu Gu Shuang Chen Hui'e Liu Yubin Wang Pingping Zhou | 2018 | Chinese Journal of Chemical Engineering2018，26，12： | 6 |
| 4 | Distribution of aluminum species and the characteristics of structure of poly-aluminum-chloride-sulfate(PACS)显示文摘A series of poly\|aluminum\|chloride\|sulfate (PACS), which has different basicities (γ) and Al 3+ /SO 2- 4 molar ratio, has been prepared and dried at 105℃ and 65℃, respectively. The distribution of aluminum species of PACS was examined, and the effect of γ value, Al 3+ /SO 2- 4 molar ratio, dilution on the distribution of aluminum species of PACS was also investigated by using Al\|ferron timed complex colorimetric method. The IR spectroscopy and X \|ray diffraction were used to study the effect of γ value, Al 3+ /SO 2- 4 molar ratio and the drying temperature on the structure of PACS. The experimental results show that Al 3+ /SO 2- 4 molar ratio has a great effect on the distribution of aluminum species, but the dilution has a little effect on the distribution of aluminum species. The lower the Al 3+ /SO 2- 4 molar ratio, the higher the proportions of the polymer and colloidal species in PACS. The polymeric degree of PACS was related to γ value and Al 3+ /SO 2- 4 molar ratio. Drying temperature has an influence on the structure and the solubility of solid PACS products. | GAO Bao\|yu, YUE Qin\|yan, YU Hui, WANG Yan (Department of Environmental Engineering, Shandong University, Jinan\ 250100, China. E\|mail:bygao@sdu.edu.cn) | 2001 | Journal of Environmental Sciences2001，13，1： | 5 |
| 5 | A Novel Intrusion Detection Method Based on Improved SVM by Combining PCA and PSO显示文摘The paper presents an improved support vector machine (SVM) by combining principal component analysis (PCA) and particle swarm optimization (PSO).Then,the improved SVM is applied to the intrusion detection system (IDS) to improve the detection rate.First,PCA is used to reduce the dimension of feature vectors.Second,we use the PSO algorithm to optimize the punishment factor C and kernel parameters in SVM.The experimental results indicate that the intrusion detection rate (97.752 8%) of improved SVM by combining PCA and PSO is higher than those (95.635 5%) of PSO-SVM and those (90.476 2%) of standard SVM with KDD Cup 1999 data set. | WANG Hui ZHANG Guilingi E Mingjie SUN Na | 2011 | Wuhan University Journal of Natural Sciences2011，16，5： | 5 |
| 6 | A comparison of surgical efficacy between a 1.8-mm microincision and 3.2-mm and 5.5-mm incisions for phacoemulsification显示文摘Phacoemulsification is a commonly used surgical method in cataract surgery. This paper observes and compares the surgical efficacy of three incisions of different length for phacoemulsification to identify the optimal method for cataract surgery. Ninety patients were enrolled in the present study and divided into three groups. The 1.8-mm group received Bausch & Lomb MI60 foldable intraocular lens(IOL) implantation(n=30), 3.2-mm group received Bausch & Lomb Akreos AO foldable lens implantation(n=30), and 5.5-mm group received Alcon TYPE 05 rigid IOL implantation(n=30). Visual acuity, Oculyzer-based anterior segment analysis, and corneal endothelial cell count before surgery, and 3, 7, 30, and 90 d after surgery were recorded and compared. Pseudophakic accommodation three days, one week, one month, and three months after surgery was determined. Intraoperative ultrasound time and ultrasonic energy were recorded. It was finally concluded that for phacoemulsification with the same phaco tip, a 1.8-mm microincision can lead to quicker recovery of visual acuity, more stable astigmatism, and higher pseudophakic accommodation than conventional incision. | E Song Xin Li Ming-Chao Bi Hua Ren Dan Wang Zhi-Hua Cui Wei Yang Hui Shi Ya-Bin Sun | 2018 | International Journal of Ophthalmology(English edition)2018，11，3： | 4 |
| 7 | Tick-borne Pathogens in Shanxi Province, China显示文摘Ticks are recognized as one of the most important vectors that cause diseases in animals and humans,and have significant public health impacts worldwide[1].They have a wide range of hosts among terrestrial vertebrates,including mammals,birds,amphibians.The numerous zoonotic pathogens transmitted by ticks are the causative agents of Lyme disease,rickettsiosis,ehrlichioses,relapsing fever,Q fever,and tularemia.Cases of tick-borne diseases are increasing around the world and some emerging or re-emerging pathogens are being discovered,which pose great challenges for prevention and control. | YANG Xiao Na YANG Hui Jun ZHANG Lin HOU Xue Xia MIAO Guang Qing CAO Hong Bing HAO Rui E HAO Qin | 2021 | Biomedical and Environmental Sciences2021，34，5： | 4 |
| 8 | Celiac disease serology in patients with different pretest probabilities: Is biopsy avoidable?显示文摘AIM: To establish the diagnostic performance of sev-eral serological tests, individually and in combination, for diagnosing celiac disease (CD) in patients with different pretest probabilities, and to explore potential se- rological algorithms to reduce the necessity for biopsy. METHODS: We prospectively performed duodenal biopsy and serology in 679 adults who had either high risk (n = 161) or low risk (n = 518) for CD. Blood samples were tested using six assays (enzyme-linked immunosorbent assay) that detected antibodies to tissue transglutaminase (tTG) and deamidated gliadin peptide (DGP). RESULTS: CD prevalence was 39.1% in the high-risk population and 3.3% in the low-risk group. In high-risk patients, all individual assays had a high diagnostic efficacy [area under receiving operator characteristic curves (AU ROC): 0.968 to 0.999]. In contrast, assays had a lower diagnostic efficacy (AU ROC: 0.835 to 0.972) in the low-risk group. Using assay combinations, it would be possible to reach or rule out diagnosis of CD without biopsy in 92% of cases in both pretest populations. We observed that the new DGP/tTG Screen assay resulted in a surplus compared to more conventional assays in any clinical situation. CONCLUSION: The DGP/tTG Screen assay could be considered as the best initial test for CD. Combinations of two tests, including a DGP/tTG Screen, might be able to diagnose CD accurately in different clinical scenarios making biopsy avoidable in a high proportion of subjects. | Emilia Sugai María L Moreno Hui J Hwang Ana Cabanne Adriana Crivelli Fabio Nach-man Horacio Vázquez Sonia Niveloni Julio Argonz Roberto Mazure Graciela La Motta María E Caniggia Edgardo Smecuol Néstor Chopita Juan C Gómez Eduardo Maurińo Julio C Bai | 2010 | World Journal of Gastroenterology2010，16，25： | 4 |
| 9 | OsbZIP76 interacts with OsNF-YBs and regulates endosperm cellularization in rice (Oryza sativa)显示文摘Following double fertilization,plant endosperm nuclei undergo syncytial divisions,followed by synchro-nous cellularization.Cellularization is a key event during endosperm development,but our understanding of its regulation is limited to Arabidopsis.In this study we show that OsbZIP76 regulates cellularization in rice(Oryza sativa).Activation of OsbZIP76 coincided with the initiation of cellularization,and its knockdown or knockout mutants exhibited precocious cellularization.Genes involved in en-dosperm development or starch biosynthesis were pre-maturely activated in the osbzip76 caryopsis.As a putative transcription factor,OsbZIP76 alone lacked transcriptional activation activity;however,it interacted with the nuclear factor Y(NF-Y)family transcription factors OsNF-YB9 and OsNF-YB1 in yeast and in planta.OsbZIP76 and OsNF-YB9 were predominantly expressed in the endosperm and the proteins colocalized.Seeds of osnf-yb1 and osbzip76 mu-tants showed reduced size and reduced apparent amylose content.The parent-of-origin-dependent expression of OsbZIP76 is variable in different rice accessions.In sum-mary,OsbZIP76 is an endosperm-expressed imprinted gene that regulates endosperm development in rice. | Baixiao Niu Hui Deng Tingting Li Sandeep Sharma Qianbin Yun Qianru Li Zhiguo E Chen Chen | 2020 | Journal of Integrative Plant Biology2020，62，12： | 4 |
| 10 | Development and calibration of the Moon-based EUV camera for Chang'e-3显示文摘The process of development and calibration for the first Moon-based extreme ultraviolet(EUV) camera to observe Earth's plasmasphere is introduced and the design, test and calibration results are presented. The EUV camera is composed of a multilayer film mirror, a thin film filter, a photon-counting imaging detector, a mechanism that can adjust the direction in two dimensions, a protective cover, an electronic unit and a thermal control unit. The center wavelength of the EUV camera is 30.2 nm with a bandwidth of 4.6 nm. The field of view is 14.7° with an angular resolution of 0.08°, and the sensitivity of the camera is 0.11 count s-1Rayleigh-1. The geometric calibration, the absolute photometric calibration and the relative photometric calibration are carried out under different temperatures before launch to obtain a matrix that can correct geometric distortion and a matrix for relative photometric correction,which are used for in-orbit correction of the images to ensure their accuracy. | Bo Chen Ke-Fei Song Zhao-Hui Li Qing-Wen Wu Qi-Liang Ni Xiao-Dong Wang Jin-Jiang Xie Shi-Jie Liu Ling-Ping He Fei He Xiao-Guang Wang Bin Chen Hong-Ji Zhang Xiao-Dong Wang Hai-Feng Wang Xin Zheng Shu-Lin E Yong-Cheng Wang Tao Yu Liang Sun Jin-Ling Wang Zhi Wang Liang Yang Qing-Long Hu Ke Qiao Zhong-Su Wang Xian-Wei Yang Hai-Ming Bao Wen-Guang Liu Zhe Li Ya Chen Yang Gao Hui Sun Wen-Chang Chen | 2014 | Research in Astronomy and Astrophysics2014，14，12： | 4 |
| 11 | EECP in the treatment of endothelial dysfunction: preventing progression of cardiovascular disease显示文摘 | John CK Hui William E Lawson Gregory W Barsness | 2010 | Journal of Geriatric Cardiology2010，7，2： | 3 |
| 12 | Tracking of CFSE-labeled endothelial progenitor cells in laser-injured mouse retina显示文摘背景 Endothelial 祖先房间(EPC ) 移植是为 ischemic retinopathy 的有希望的治疗学的策略。当前的学习试图为与 5-(and-6 ) 追踪 EPC 建立一个简单、可靠、荧光灯的标记的方法在伤害激光的老鼠 retina.Methods EPC 的 -carboxyfluorescein diacetate succinimidyl 酉旨(CFSE ) 从人的脐的绳索血 mononuclear 房间被孤立,栽培,并且用 CFSE 的各种各样的集中标记。基于荧光紧张和房间形态学,有在 37 点的 5 mol/L CFSE 的 15 分钟孵化作为最佳的标记条件被选择。幸存能力和标记 CFSE 的 EPC 的 apoptosis 率被染色的 Trypan 蓝色和 Annexin V/PI 染色试金分别地测量。荧光显微镜学被用来在扩大文化时期期间观察标签稳定性。标记的 EPC 被移植进网膜的激光光致疑结伤害的 pigmented 老鼠的玻璃的洞。Evans 蓝 angiography 和完全装的视网膜被检验追踪用 5 mol/L CFSE 介绍了标记的标记的 cells.Results EPC 一块强烈草地荧光和维持的正常形态学没有在在被标记 2 天以后的幸存能力或 apoptosis 率的重要变化, 1 和 4 个星期。荧光紧张逐渐地在 4 个星期的结束在房间减少了。视网膜的 Evans 蓝 angiography 清楚地显示了网膜的毛细作用网络,荧光漏在伤害激光的老鼠模型在 photocoagulated 点附近被观察。在标记的 EPC 的移植以后的一个星期,荧光灯房间在 photocoagulated 损害附近被识别。在移植以后的四个星期,荧光灯像试管的结构在网膜的脉管的 networks.Conclusion EPC 被观察能被 CFSE 在 vitro 标记并且在 vivo 监视了至少 4 个星期,并且参予受伤网膜的容器的修理。 | SHI Hui YANG Wei CUI Zhi-hua LU Cheng-wei LI Xiao-hong LIANG Ling-ling SONG E | 2011 | Chinese Medical Journal2011，，5： | 3 |
| 13 | Simulating global soil-CO_2 flux and its response to climate change显示文摘It has been argued that increased soil respiration would become a major atmospheric source of CO\-2 in the event of global warming. The simple statistical models were developed based on a georeferenced database with 0 5°×0 5° longitude/latitude resolution to simulate global soil\|CO\-2 fluxes, to investigate climatic effects on these fluxes using sensitivity experiments, and to assess possible responses of soil\|CO\-2 fluxes to various climate change scenarios. The statistical models yield a value of 69 PgC/a of global soil\|CO\-2 fluxes for current condition. Sensitivity experiments confirm that the fluxes are responsive to changes in temperature, precipitation and actual evapotranspiration, but increases in temperature and actual evapotranspiration affect soil\|CO 2 fluxes more than increases in precipitation. Using climatic change projections from four global circulation models, each corresponding to an equilibrium doubling of CO 2, it can be found that the largest increases in soil\|CO 2 fluxes were associated with the boreal and tundra regions. The globally averaged soil\|CO 2 fluxes were estimated to increase by about 35% above current values, providing a positive feedback to the greenhouse effect. | PENG Chang\|hui 1* , Michael J. Apps\+2 (1 Ontario Forest Research Institute, Ministry of Natural Resources, 1235 Queen Street East, Sault Ste. Marie, ON P6A 2E5,Canada. E\|mail: pengc@gov.on.ca 2 Morthern Forestry Center, Canadian Forest Serv | 2000 | Journal of Environmental Sciences2000，12，3： | 2 |
| 14 | Experimental study on dispersive soil in western Jilin显示文摘In order to research engineering geological properties of the soil in Zhenlai of western Jilin,especially the dispersivity of soil,the authors carried out the basic physicochemical test and dispersivity identification test.The results show that the dispersivity of the soil increases with the increase of depth within 0-30 cm (surface soil);it decreases as the depth increases within in 30-100 cm.Furthermore,the statistical analysis of the dispersivity indexes and physicochemical propertity indexes show that the DP is positively linear correlated with total soluble salt content,sodium ion content,ESP,pH and organic matter content.Meanwhile,it is negatively linear correlated with clay content,and the linear relationship is better.Through the study of the dispersion mechanism of soil samples,it can be concluded that sodium montmorillonite,higher percentage of exchangeable sodium and high pH are the main reasons for the dispersion of soils in western Jilin. | ZHANG Jing WANG Qing WANG Wenhua CHEN Hui'e WANG Ying | 2010 | Global Geology2010，13，1： | 2 |
| 15 | Effect of endothelial progenitor cells derived from human umbilical cord blood on oxygen-induced retinopathy in mice by intravitreal transplantation显示文摘·AIM: To investigate the effect of endothelial progenitor cells(EPCs) labeled by carboxy fluorescein diacetate succinimidyl ester(CFSE) on murine oxygen-induced retinopathy(OIR) by intravitreal transplantation.·METHODS: After isolated from human umbilical cord blood mononuclear cells, EPCs were cultivated and then labeled with CFSE in vitro. C57BL/6J mice were placed to75% hyperoxia chamber from P7 to P12 to establish OIR model. At P12, OIR mice were intravitreally injected with1 μL suspension contained 2×105EPCs(EPCs group) or isometric phosphate buffered saline(PBS group). The contralateral eye of each mice received no injection(OIR group). Evans blue angiography and frozen section were examined to track the labeled cells in OIR group at P15 and P19. Using retina paraffin sections and adenosinediphos phatase staining at P12 and P19, the effect of EPCs on OIR mice was evaluated quantitatively and qualitatively.· RESULTS: The retinas from EPCs group with less non-perfusion area and fewer peripheral tufts wereobserved at P19, comparing with that from PBS or OIR group. The retinopathy in EPCs group receded earlier with less non-ganglion cells and neovascular nuclei,together with relatively regular distribution. The counts of the neovascular nuclei at P19 were reduced by 44% or45%, compared with those of OIR group or PBS group respectively. Three days after EPCs injection, a large number of EPCs appeared in the vitreous cavity and adhered to the retinal surface. While at one week, the cells gathered between the internal plexiform layer and the inner limiting membrane, and some EPCs appeared in retinal vessels.·CONCLUSION: EPCs transplantation can participate in the reparative procedure of the neovascularization in OIR. | Dan Wang Bo Zhang Hui Shi Wei Yang Ming-Chao Bi Xiang-Fu Song Chen Zhang Jian-Hui Cheng Ji-Long Hao E Song | 2016 | International Journal of Ophthalmology(English edition)2016，9，11： | 2 |
| 16 | Goldmanlmage seg-mentation evaluation: A survey of unsupervised methods 显示文摘 | ZHANG Hui JASON E FRITTS B | 2008 | Computer Vision anti Image Understandin2008，110，2： | 1 |
| 17 | Oxidation state of gold and arsenic in gold-hearing arsenian pyrites 显示文摘 | Simon G Huang Hui Penner J E | 1999 | American Mineralogist1999，84，： | 1 |
| 18 | Measuring customer satisfaction of FM service in housing sector:A structural equation model approach显示文摘 | HUI E C M ZHENG X | | 0，，5： | 1 |
| 19 | Quantitative analysis and prognostic implication of SARS coronavirus RNA in the plasma and serum of patients with severe acute respiratory syndrome 显示文摘 | Ng E K Hui D S Chan K C | 2003 | Clin Chem2003，49，12： | 1 |
| 20 | Image segmentation evaluation: A survey of unsupervised methods显示文摘 | ZHANG Hui FRITTS J E GOLDMAN S A | 2008 | Computer Vision and Image Understanding2008，110，2： | 1 |
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