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| 1 | Effect of hydrophilic silica nanoparticles on hydrate formation: Insight from the experimental study显示文摘Invasion of drilling fluid into natural gas hydrate deposits during drilling might damage the reservoir,induce hydrate dissociation and then cause wellbore instability and distortion of the data from well logging. Adding nanoparticles into drilling fluid is an effective method in reducing the invasion of drilling fluid and enhancing borehole stability. However, the addition of nanoparticles might also introduce hydrate formation risk in borehole because they can act as the 'seeds' for hydrate nucleation. This paper presents an experimental study of the effect of hydrophilic silica nanoparticle on gas hydrate formation in a dynamic methane/liquid-water system. In the experiment, the ultrapure water with and without1.0 wt%–6.0 wt% concentrations of silica nanoparticles, grain sizes of 20 and 50 nm, were pressurized by methane gas under varied conditions of temperature and pressure. The induction time, the gas consumption, and the average rate of gas consumption in the system were measured and compared to those in ultrapure water. The results show that a concentration of 4.0 wt% hydrophilic SiO_2 particles with a grain size of 50 nm has a relatively strong inhibition effect on hydrate formation when the initial experimental condition is 5.0 °C and 5.0 MPa. Compared to ultrapure water, the hydrophilic nano-SiO_2 fluid increases the induction time for hydrate formation by 194% and decreases the amount and average rate of hydrate formation by 10% and 17%, respectively. This inhibition effect may be attributed to the hydrophilicity,amount and aggregation of silica nanoparticle according to the results of water activity and zeta potential measurements. Our work also elucidates hydrophilic, instead of hydrophobic, nanoparticles can be added to the drilling fluid to maintain wellbore stability and to protect the hydrate reservoir from drilling mud damage, because they exhibit certain degree of hydrate inhibition which can reduce the risk of hydrate reformation and aggregation during gas hydrate or deep water drilling if their concentration can be controlled properly. | Ren Wang Tianle Liu Fulong Ning Wenjia Ou Ling Zhang Zhen Wang Li Peng Jiaxin Sun Zhichao Liu Tianshu Li Huicui Sun Guosheng Jiang | 2019 | Journal of Energy Chemistry2019，28，3： | 12 |
| 2 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 3 | Ecosystem carbon storage and sink/source of temperate forested wetlands in Xiaoxing’anling, northeast China显示文摘Wetlands play an important role in the global carbon cycle, but there are still considerable uncertainties in the estimation of wetland carbon storage and a dispute on whether wetlands are carbon sources or carbon sinks. Xiaoxing’anling are one of several concentrated distribution areas of forested wetland in China, but the carbon storage and carbon sink/source of forested wetlands in this area is unclear. We measured the ecosystem carbon storage (vegetation and soil), annual net carbon sequestration of vegetation and annual carbon emissions of soil heterotrophic respiration of five typical forested wetland types (alder swamp, white birch swamp, larch swamp, larch fen, and larch bog) distributed along a moisture gradient in this area in order to reveal the spatial variations of their carbon storage and quantitatively evaluate their position as carbon sink or source according to the net carbon balance of the ecosystems. The results show that the larch fen had high carbon storage (448.8 t ha^(−1)) (6.8% higher than the larch bog and 10.5–30.1% significantly higher than other three wetlands (P < 0.05), the white birch swamp and larch bog were medium carbon storage ecosystems (406.3 and 420.1 t ha^(−1)) (12.4–21.8% significantly higher than the other two types (P < 0.0 5), while the alder swamp and larch swamp were low in carbon storage (345.0 and 361.5 t ha^(−1), respectively). The carbon pools of the five wetlands were dominated by their soil carbon pools (88.5–94.5%), and the vegetation carbon pool was secondary (5.5–11.5%). At the same time, their ecosystem net carbon balances were positive (0.1–0.6 t ha^(−1) a^(−1)) because the annual net carbon sequestration of vegetation (4.0–4.5 t ha^(−1) a^(−1)) were higher than the annual carbon emissions of soil heterotrophic respiration (CO_(2) and CH_(4)) (3.8–4.4 t ha^(−1) a^(−1)) in four wetlands, (the alder swamp being the exception), so all four were carbon sinks while only the alder swamp was a source of carbon emissions (− 2.1 t ha^(−1) a^(−1)) due to a degraded tree layer. Our results demonstrate that these forested wetlands were generally carbon sinks in the Xiaoxing’anling, and there was obvious spatial variation in carbon storage of ecosystems along the moisture gradient. | Biao Wang Changcheng Mu Huicui Lu Na Li Yan Zhang Li Ma | 2022 | Journal of Forestry Research2022，33，3： | 2 |
| 4 | Versatile synthesis of functional biodegradable polymers by combining ring-opening polymerization and postpolymerization modification via michael-type addition reaction显示文摘 | Chen Wei Yang Huicui Wang Rong | 2010 | Macromolecules2010，43，1： | 1 |
| 5 | Human Elongator complex is involved in cell cycle and suppresses cell growth in 293T human embryonic kidney cells显示文摘Elongator 建筑群与 hyperphosphorylated RNA 聚合酶 II 被联系了并且被知道在 transcriptional 延伸,以及在 tRNA 修正和 exocytosis 起关键作用。然而,人的 Elongator 建筑群怎么调整细胞生长和细胞周期的特定的机制仍然保持不清楚。在房间生长上调查人的 Elongator 建筑群和它的效果的作文, 293T 房间被建立那稳定地 overexpressed Flag-Elp3 和 Flag-Elp4。由使用反旗帜 M2 抗体界限树脂,核心 Elongator 建筑群从房间被净化那稳定地 overexpressed Flag-Elp3。没有 Elongator 建筑群稳定地从房间被净化有 pFlagCMV4-Elp4 的 transfected。有趣地,细胞生长与 pFlagCMV4-Elp3 在 293T 细胞 transfected 被禁止。流动 cytometry 分析证明大多数稳定地, overexpressing Flag-Elp3 在 G1 被发现的房间上演,显示在为房间周期的规定的 G1 检查点的核心 Elongator 的一个角色。我们观察到增加的基础抄写和显著地提高的抄写在 293T 房间 overexpressing Flag-Elp3 由 VP16 刺激了。抄写能被 overexpressing synergistically 也激活 Elp3 和 Elp4。一起拿,而 Elp4, Elp5,和 Elp6 可能泛泛地联系并且和核心 Elongator 工作调整房间功能,我们的结果建议 Elp1, Elp2,和 Elp3 形成的核心 Elongator 建筑群是相当稳定的。 | Junxia Gu Dongmei Sun Qiping Zheng Xiaochun Wang Huicui Yang Jingcheng Miao Jingting Jiang Wenxiang Wei | 2009 | Acta Biochimica et Biophysica Sinica2009，41，10： | 1 |
| 6 | Variable length LMS adaptive filter for pseudorange multipath mitigation 显示文摘 | LIU HUICUI LI XIAOJING GE LINLIN | 2011 | GPS Solutions2011，15，1： | 1 |
| 7 | Changes in glyoxal and methylglyoxal content in the fried dough twist during frying and storage显示文摘 | Huicui Liu Juxiu Li | 2014 | European Food Research and Technology2014，，2： | 1 |
| 8 | Hypoxia-inducible factor upregulation by roxadustat attenuates drug reward by altering brain iron homoeostasis显示文摘Substance use disorder remains a major challenge,with an enduring need to identify and evaluate new,translational targets for effective treatment.Here,we report the upregulation of Hypoxia-inducible factor-1α(HIF-1α)expression by roxadustat(Rox),a drug developed for renal anemia that inhibits HIF prolyl hydroxylase to prevent degradation of HIF-1α,administered either systemically or locally into selected brain regions,suppressed morphine(Mor)-induced conditioned place preference(CPP). | Pengju Yan Ningning Li Ming Ma Zhaoli Liu Huicui Yang Jinnan Li Chunlei Wan Shuliu Gao Shuai Li Longtai Zheng John LWaddington Lin Xu Xuechu Zhen | 2023 | Signal Transduction and Targeted Therapy2023，8，10： | 0 |
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