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| 1 | A regeneration system using cotyledons and cotyledonary node explants of Toona ciliata显示文摘We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants. | Huiyun Song Wenmai Mao Yuanyuan Shang Wei Zhou Pei Li Xiaoyang Chen | 2021 | Journal of Forestry Research2021，32，3： | 2 |
| 2 | Extensive expansion of the chemical diversity of fusidane-type antibiotics using a stochastic combinational strategy显示文摘Fusidane-type antibiotics,represented by helvolic acid,fusidic acid and cephalosporin P1,are fungi-derived antimicrobials with little cross-resistance to commonly used antibiotics.Generation of new fusidane-type derivatives is therefore of great value,but this is hindered by available approaches.Here,we developed a stochastic combinational strategy by random assembly of all the post-tailoring genes derived from helvolic acid,fusidic acid,and cephalosporin P1 biosynthetic pathways in a strain that produces their common intermediate.Among a total of 27 gene combinations,24 combinations produce expected products and afford 58 fusidane-type analogues,of which 54 are new compounds.Moreover,random gene combination can induce unexpected activity of some post-tailoring enzymes,leading to a further increase in chemical diversity.These newly generated derivatives provide new insights into the structure-activity relationship of fusidane-type antibiotics.The stochastic combinational strategy established in this study proves to be a powerful approach for expanding structural diversity of natural products. | Xiaojun Song Jianming Lv Zhiqin Cao Huiyun Huang Guodong Chen Takayoshi Awakawa Dan Hu Hao Gao Ikuro Abe Xinsheng Yao | 2021 | Acta Pharmaceutica Sinica B2021，11，6： | 1 |
| 3 | CmMLO17 and its partner CmKIC potentially support Alternaria alternata growth in Chrysanthemum morifolium显示文摘The Mildew Resistance Locus O(MLO)gene family has been investigated in many species.However,there are few studies on chrysanthemum MLO genes.We report in this study that CmMLO17 in Chrysanthemum morifolium was upregulated after Alternaria alternata infection.Silencing of CmMLO17 by artificial microRNA resulted in reduced susceptibility of chrysanthemum to A.alternata infection.Genes in the abscisic acid(ABA)and Ca 2+signaling pathways were upregulated in the CmMLO17-silenced line R20 compared to the wild-type plants.We speculated that CmMLO17-silenced plants had a faster and stronger defense response that was mediated by the ABA and Ca 2+signaling pathways,resulting in reduced susceptibility of chrysanthemum to A.alternata infection.In addition,a candidate gene,CmKIC,that may interact with CmMLO17 was discovered by the yeast two-hybrid assay.The interaction between CmMLO17 and CmKIC was confirmed using the yeast two-hybrid assay and bimolecular fluorescence complementation(BiFC)analysis.CmMLO17 and CmKIC were both located on the plasma membrane,and CmKIC was also located on the nucleus.CmKIC overexpression increased the susceptibility of chrysanthemum to A.alternata,whereas CmKIC silencing resulted in reduced susceptibility.Therefore,CmMLO17 and CmKIC may work together in C.morifolium to support the growth of A.alternata.The results of this study will provide insight into the potential function of MLO and improve the understanding of plant defense responses to necrotrophic pathogens. | Jingjing Xin Ye Liu Huiyun Li Sumei Chen Jiafu Jiang Aiping Song Weimin Fang Fadi Chen | 2021 | Horticulture Research2021，8，1： | 1 |
| 4 | Nonfatal unintentional injuries and related factors among male construction workers in Central China显示文摘 | Lei Zheng Huiyun Xiang Xiaoqin Song | | American Journal of Industrial Medicine0，，： | 1 |
| 5 | Variation for Resistance to Alternaria tenuissima and Potential Structural Mechanism among Different Cultivars of Chrysanthemum morifolium显示文摘Black spot disease,caused by the necrotrophic fungus Alternaria tenuissima(Fr.)Wiltsh(A.tenuissima),is considered a highly destructive disease of Chrysanthemum(Chrysanthemum morifolium Ramat.).A set of 17 accessions of commercial chrysanthemum cultivars were evaluated for resistance to A.tenuissima by seedling artificial inoculation.It was found that the reaction of the accessions to artificial inoculation ranged from resistant to highly susceptible.Five varieties of chrysanthemum(‘Zhongshan Taogui’,‘Jinba’,‘Zhongshan Jinguan’,‘Jinling Wanhuang’and‘Jinling Yangguang’)were resistant;two varieties of chrysanthemum(‘Zhongshan Xinggui’and‘Zhongshan Jinkui’)were moderately resistant;and others were susceptible to various degrees,four varieties of chrysanthemum(‘Zhongshan Zihe’,‘Zhongshan Jiuhong’,‘Zaoyihong’and‘Jinling Jiaohuang’)were highly susceptible,especially.Some leaf morphological features of two resistant and two highly susceptible cultivars were further researched.Trichome density,length,height,gland size and stomata density were found to be associated with plant passive resistance.Resistant varieties that were identified in present study will be promising germplasm for exploitation of breeding programmes aimed at developing A.tenuissima-resistant cultivars and increasing genetic diversity. | Huiyun Li Ye Liu Sumei Chen Jiafu Jiang Aiping Song Weimin Fang Fadi Chen | 2020 | Phyton-International Journal of Experimental Botany2020，89，4： | 0 |
| 6 | Geographic variation in growth and wood traits of Neolamarckia cadamba in China显示文摘Neolamarckia cadamba is an indigenous,timber-producing tree species in Southern China that plays an important role in the sustainable development of the local forestry industry.However,the geographic genetic variation across its natural distribution area in Southern China has yet to be characterized for best utilization.Here,we report the geographic genetic variation in growth and wood properties of N.cadamba from 10 provenances that represent the entire natural distribution of N.cadamba in Southern China.There was significant geographic variation in diameter breast height(DBH),height(H),volume(V),vessel length(VL),vessel diameter(VD),VL/VD,and wood basic density(WBD).The variation in tree volume across provenances was greater than that of other growth traits,indicating that volume has a greater potential for selection in provenance trials.The provenance heritabilities of growth traits and wood properties ranged from 0.59 to 0.67 and from 0.40 to 0.45,respectively.Trend surface analysis revealed that patterns of geographic variation associated with growth traits were weakly negatively correlated with those of wood properties.The pattern of geographic variation in growth traits showed a gradual increase from the periphery to the central region,whereas wood properties showed the opposite pattern,and latitude had the greatest effect on both.Wood property measurements suggested that the YNMS provenance produced superior timber wood,whereas the GXLZ,GXFCG,and GXNN provenances produced the best pulpwood.These provenances could potentially provide more valuable breeding materials for the genetic improvement of N.cadamba. | Qingmin Que Kunxi Ouyang Chunmei Li Buye Li Huiyun Song Pei Li Ruiqi Pian Huaqing Li Xiaoyang Chen Changcao Peng | 2022 | Forestry Research2022，2，1： | 0 |
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