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| 1 | 施用有机肥对侵蚀黑土玉米苗期根内生细菌多样性的影响显示文摘为了解根内生细菌群落结构在土壤侵蚀条件下的变化,明确有机肥施用对其多样性的影响,本研究采用LNA-PCR和454高通量测序技术相结合的方法对土壤表层剥离30 cm和施用有机肥处理的玉米苗期根内生细菌群落结构进行研究,以表层土没有剥离和单施化肥处理为对照.试验共获得37820条16S r DNA有效序列,主要分布在4个门35个纲214个属和782个OTU,其中,变形菌门、厚壁菌门、放线菌门和拟杆菌门为优势菌门,但不同样品各菌门分布比例存在差异.土壤侵蚀降低了玉米苗期根内生细菌群落多样性,但增施有机肥可以增加根内生细菌群落多样性,这种作用以表层土剥离30 cm处理表现得更为突出. | 喻江 于镇华 IKENAGA Makoto SAKAI Masao 刘晓冰 王光华 | 2016 | 应用生态学报2016，27，8： | 9 |
| 2 | Characterization of root-associated bacterial community structures in soybean and corn using locked nucleic acid(LNA) oligonucleotide-PCR clamping and 454 pyrosequencing显示文摘The community structure and diversity of root-associated bacteria have been tentatively investigated using polymerase chain reaction(PCR) amplification methods in several studies.However,the homology between small submit ribosomal(SSU) rRNA genes of plant plastids and mitochondria and that of bacteria have hindered in these studies.To address this issue,in this paper,we adopted the methods of locked nucleic acid(LNA) oligonucleotide-PCR clamping with 454 pyrosequencing to analysis the root-associated bacterial community compositions in soybean and corn.Results showed that plant chloroplast and mitochondria genes were effectively inhibited from PCR amplification in the root samples with LNA oligonucleotides(LNA(+)),and PCR amplicons with LNA(+) had higher bacterial operational taxonomic unit(OTU)numbers and ACE,Chao1,and Shannon indices,as well as a lower Simpson index than the corresponding samples without LNA oligonucleotides(LNA(-)).Those findings suggested that the methods of this study provide a much more detail description of root-associated bacterial communities.In the soybean LNA(+) sample,Pseudomonas,Bradyrhizobium and Flavobactehum were the three most abundant genera,whereas the top two predominant genera in corn LNA(+) samples were Streptomyces and Niastella.The presence and absence of major genera varied between soybean and corn,suggesting the root-associated bacterial communities differed between two crops.The rare phylotypes and uncultured root-associated bacterial members detected in this study inferred that the root-associated bacterial communities are highly complex and information on their taxonomic affiliates potentially gives the clues for selecting the optimal medium and method to isolate the novel bacteria for further functional analysis. | YU Zhen-hua YU Jiang Makoto Ikenaga Masao Sakai LIU Xiao-bing WANG Guang-hua | 2016 | Journal of Integrative Agriculture2016，15，8： | 6 |
| 3 | A heat-gearating bioactive glass-ceramic for hyperthermia显示文摘 | Ohura K Ikenaga M Nakamura T | 1991 | J Appl Biomater1991，2，： | 2 |
| 4 | Production of hydrogen by steam reforming of glycerin on ruthenium catalyst 显示文摘 | Hirai T Ikenaga N O Miyake T | 2005 | Energy and fuels2005，9，： | 1 |
| 5 | Co-hquefaction of MicroAlgae with Coal Using Coal Liquefaction Catalysts显示文摘 | IKENAGA N UEDA C MATSUI T | | 0，，02： | 1 |
| 6 | 显示文摘 | Okamura M Ikenaga N | 1998 | Chem Commun1998，1025，： | 1 |
| 7 | Production of hydrogen by steam reforming of glycerin on ruthenium catalyst显示文摘 | Hirai T Ikenaga N O Miyake T | 2005 | Energy & Fuels2005，19，4： | 1 |
| 8 | Radiculopathy of Cs after anterior decompression for cervical myelopathy 显示文摘 | Ikenaga M Shikata J Tanaka C | 2005 | J Neurosurg Spine2005，3，： | 1 |
| 9 | Dehydrogenation of Ethylbenzene with an Activated Carbon-supported Vanadium Catalyst显示文摘 | Sakurai Y Suzaki T Ikenaga N | 2000 | Appl Catal A2000，192，： | 1 |
| 10 | Production of choleretic substances in the capitulum, leaf and stem of Artemisia capillaris during plant growth 显示文摘 | Ikenaga T Hizako M Tajima M | 1994 | Biol Pharm Bull1994，17，1： | 1 |
| 11 | Value of general surgical risk models for predicting postoperative liver failure and mortality following liver surgery显示文摘 | Yoshio Haga Koji Ikejiri Hitoshi Takeuchi Masakazu Ikenaga Yasuo Wada | 2012 | J Surg Oncol2012，，7： | 1 |
| 12 | De- hydrogenation of isobutane to isobutene with iron-loaded activated carbon catalyst显示文摘 | SHIMADA H AKAZAWA T IKENAGA N | 1998 | Appl Catal A1998，168，： | 1 |
| 13 | Preparation of zinc ferrite in the presence of carbon material and its application to hot-gas cleaning 显示文摘 | Ikenaga N Ohgaito Y Matsushima H | 2004 | Fuel2004，83，6： | 1 |
| 14 | Effect of space flight on the frequency of micronuclei and expression of stress-responsive proteins in cultured mammalian cells显示文摘 | IKENAGA I HIRAYAMA J KATO T | 2002 | J Radiate Res2002，43，： | 1 |
| 15 | Coal hydroliquefaction using highly dispersed catalyst precursors显示文摘 | IKENAGA N KAN-NAN S SAKODA T SUZUKI T | 1997 | Catal Today1997，39，1： | 1 |
| 16 | CD10+ pancreatic stellate cells enhance the progression of pancreatic cancer显示文摘 | Ikenaga N Ohuchida K Mizumoto K | 2010 | Gastroenterology2010，139，3： | 1 |
| 17 | microRNA-203 expression as a new prognostic marker of pancreatic adenocarcinoma显示文摘 | Ikenaga N Ohuchida K Mizumoto K | 2010 | Ann Surg Oncol2010，17，12： | 1 |
| 18 | A contour-based robust algorithm for text detection in color images 显示文摘 | Yangxing L I U Ikenaga T | 2006 | IEICE Transactions on Information and Systems2006，89，3： | 1 |
| 19 | 显示文摘 | Kajita C Ikenaga N | 2003 | J Phys Chem B2003，107，： | 1 |
| 20 | Development-inhibiting of some tropical plants against Aitophilus zeamais Motschulsky(Coleoptera:Curulionidae)显示文摘 | Haque MA Nakakita H Ikenaga H | 2000 | Journal of Stored Products Research2000，36，： | 1 |
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