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| 1 | Comprehensive screening and selection of okra (Abelmoschus esculentus) germplasm for salinity tolerance at the seedling stage and during plant ontogeny显示文摘The okra germplasm was screened for salinity tolerance at the seedling stage and during plant ontogeny.Substantial variation existed in okra for salinity tolerance at the seedling stage.An 80 mmol/L NaCl concentration was suitable for discriminating tolerant and non-tolerant okra genotypes.The pooled ranking of the genotypes,based on individual rankings for each trait(root and shoot length,germination percentage,and relative Na+ and K+)in individual NaCl concentrations,was effective for selecting tolerant genotypes.Genotypes selected at the seedling stage maintained their tolerance to NaCl during plant ontogeny,suggesting that screening of the germplasm entries and advanced breeding materials for salt tolerance at the seedling stage is effective.Among 39 okra genotypes,five were identified as the most tolerant genotypes and showed potential for use in breeding programs that focus on the development of salt-tolerant,high-yield okra cultivars. | Ikram-ul-HAQ Asif Ali KHAN Iqrar Ahmad KHAN Muhammad Abubakkar AZMAT | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，7： | 4 |
| 2 | Genetic effects conferring heat tolerance in upland cotton(Gossypium hirsutum L.)显示文摘Background:Climate change and particularly global warming has emerged as an alarming threat to the crop productivity of field crops and exerted drastic effects on the cropping patterns.Production of cotton has been dropped down to one million bales from 1.4 million bales since 2015 in Pakistan due to the increase in temperature at critical growth stages,i.e.,flowering and boll formation.Keeping in view the importance of cotton in the country,this study was conducted to investigate the genetic effects conferring heat tolerance in six populations(P1,P2,F1,F2,BC1 and BC2)developed from cross-1 and cross-2,i.e.,VH-282×FH-142 and DNH-40×VH-259.Results:The results revealed that cross-1 performed better in heat stress as compared with cross-2 for majority of the traits recorded.Boll weight and ginning outturn(GO-T)were highly effected under heat stress and had negative correlation with Relative cell injury(RCI).Boll weight,fiber length,fiber strength and fiber fineness were under the control of non-additive gene action,whereas RCI was controlled by additive gene effects.Lower values of genetic advance coupled with higher values of broad sense heritability for these traits except RCI confirmed the role of non-additive genetic effects.Duplicate types of epistasis were recorded for fiber strength in cross-1 in normal condition.However,complementary type of non-allelic interaction was recorded for fiber strength under normal condition,fiber fineness and RCI under heat stressed condition in cross-1.Likewise,boll weight,GOT and fiber length in populations derived from cross-2 in normal condition were also under the influence of complementary type of non-allelic interaction.Significant differences among values of mid parent and better parent heterosis for boll weight in both normal and heat stress condition provided the opportunity to cotton breeders for utilization of this germplasm for improvement of this trait through exploitation of heterosis breeding.Conclusion:Cross-1 performed better in heat stress and could be utilized for development of heat tolerant cultivar.RCI was under the influence of additive gene action,so one can rely on this trait for screening of large number of accessions of cotton for heat stress.While other traits were predominantly controlled by non-additive gene action and selection based on these should be delayed in later generations. | SALMAN Muhammad ZIA Zia Ullah RANA Iqrar Ahmad MAQSOOD Rana Haroon AHMAD Saghir BAKHSH Ali AZHAR Muhammad Tehseen | 2019 | Journal of Cotton Research2019，2，1： | 2 |
| 3 | Role of SNPs in determining QTLs for major traits in cotton显示文摘A single nucleotide polymorphism is the simplest form of genetic variation among individuals and can induce minor changes in phenotypic,physiological and biochemical characteristics.This polymorphism induces various mutations that alter the sequence of a gene which can lead to observed changes in amino acids.Several assays have been developed for identification and validation of these markers.Each method has its own advantages and disadvantages but genotyping by sequencing is the most common and most widely used assay.These markers are also associated with several desirable traits like yield,fibre quality,boll size and genes respond to biotic and abiotic stresses in cotton.Changes in yield related traits are of interest to plant breeders.Numerous quantitative trait loci with novel functions have been identified in cotton by using these markers.This information can be used for crop improvement through molecular breeding approaches.In this review,we discuss the identification of these markers and their effects on gene function of economically important traits in cotton. | MAJEED Sajid RANA Iqrar Ahmad ATIF Rana Muhammad ALI Zulfiqar HINZE Lori AZHAR Muhammad Tehseen | 2019 | Journal of Cotton Research2019，2，1： | 2 |
| 4 | Over Expression of Chitinase and Chitosanase Genes from Trichoderma harzianum under Constitutive and Inducible Promoters in order to Increase Disease Resistance in Wheat (Triticum aestivum L)显示文摘 | Iqrar AR Horst L Wilheim S | | 0，，04： | 1 |
| 5 | Surface and Solution Properties of Cationic Gemini Surfactants with Primary Linear Alkanols显示文摘 | Riyaj Mohammad Iqrar Ahmad Khan Kabir-ud-Din Pablo C. Schulz | 2013 | Journal of Solution Chemistry2013，，12： | 1 |
| 6 | Over Expression of Chitinase and Chitosanase Genes from Trichoderma harzianum under Constitutive and Inducible Promoters in order to Increase Disease Resistance in Wheat (Triticum aestivum L)显示文摘 | Iqrar A R Horst L Wilhelm S | 2012 | Molecular Plant Breeding2012，3，4： | 1 |
| 7 | Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L.显示文摘Good quality deoxyribonucleic acid (DNA) is the pre-requisite for its downstream applications. The presence of high concentrations of polysaccharides, polyphenols, proteins, and other secondary me- tabolites in mango leaves poses problem in getting good quality DNA fit for polymerase chain reaction (PCR) applications. The problem is exacerbated when DNA is extracted from mature mango leaves. A reliable and modified protocol based on the cetyl- trimethylammonium bromide (CTAB) method for DNA extraction from mature mango leaves is described here. High concentrations of inert salt were used to remove polysaccharides; Polyvinylpyrrolidone (PVP) and β-mercaptoethanol were employed to manage phenolic compounds. Extended chloroform-isoamyl alcohol treatment followed by RNase treatment yielded 950?1050 μg of good quality DNA, free of protein and RNA. The problems of DNA degradation,contamination, and low yield due to irreversible binding of phenolic compounds and coprecipitation of polysaccharides with DNA were avoided by this method. The DNA isolated by the modified method showed good PCR amplification using simple se- quence repeat (SSR) primers. This modified protocol can also be used to extract DNA from other woody plants having similar problems. | Muhammad Abubakkar AZMAT Iqrar Ahmad KHAN Hafiza Masooma Naseer CHEEMA Ishtiaq Ahmad RAJWANA Ahmad Sattar KHAN Asif Ali KHAN | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，4： | 1 |
| 8 | Genetics of biochemical attributes regulating morpho-physiology of upland cotton under high temperature conditions显示文摘Background Cotton is a strategically important fibre crop for global textile industry.It profoundly impacts several countries’industrial and agricultural sectors.Sustainable cotton production is continuously threatened by the unpre-dictable changes in climate,specifically high temperatures.Breeding heat-tolerant,high-yielding cotton cultivars with wide adaptability to be grown in the regions with rising temperatures is one of the primary objectives of modern cotton breeding programmes.Therefore,the main objective of the current study is to figure out the effective breed-ing approach to imparting heat tolerance as well as the judicious utilization of commercially significant and stress-tolerant attributes in cotton breeding.Initially,the two most notable heat-susceptible(FH-115 and NIAB Kiran)and tolerant(IUB-13 and GH-Mubarak)cotton cultivars were spotted to develop filial and backcross populations to accom-plish the preceding study objectives.The heat tolerant cultivars were screened on the basis of various morphological(seed cotton yield per plant,ginning turnout percentage),physiological(pollen viability,cell membrane thermostabil-ity)and biochemical(peroxidase activity,proline content,hydrogen peroxide content)parameters.Results The results clearly exhibited that heat stress consequently had a detrimental impact on every studied plant trait,as revealed by the ability of crossing and their backcross populations to tolerate high temperatures.However,when considering overall yield,biochemical,and physiological traits,the IUB-13×FH-115 cross went over particularly well at both normal and high temperature conditions.Moreover,overall seed cotton yield per plant exhibited a posi-tive correlation with both pollen viability and antioxidant levels(POD activity and proline content).Conclusions Selection from segregation population and criteria involving pollen viability and antioxidant levels concluded to be an effective strategy for the screening of heat-tolerant cotton germplasms.Therefore,understanding acquired from this study can assist breeders identifying traits that should be prioritized in order to develop climate resilient cotton cultivars. | MAJEED Sajid CHAUDHARY Muhammad Tanees MUBARIK Muhammad Salman RANA Iqrar Ahmad SHABAN Muhammad TAN Daniel KY JIA Yinhua DU Xiongming HINZE Lori AZHAR Muhammad Tehseen | 2024 | Journal of Cotton Research2024，7，1： | 0 |
| 9 | Comparative analysis of SIMILAR to RCD ONE(SRO)family from tetraploid cotton species and their diploid progenitors depict their significance in cotton growth and development显示文摘Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental stresses.However,genome-wide identification and functional characteri-zation of SRO genes from cotton species have not been reported so far.Results A total of 36 SRO genes were identified from four cotton species.Phylogenetic analysis divided these genes into three groups with distinct structure.Syntenic and chromosomal distribution analysis indicated uneven distribu-tion of GaSRO,GrSRO,GhSRO,and GbSRO genes on A2,D5 genomes,Gh-At,Gh-Dt,Gb-At,and Gb-Dt subgenomes,respectively.Gene duplication analysis revealed the presence of six duplicated gene pairs among GhSRO genes.In promoter analysis,several elements responsive to the growth,development and hormones were found in GhSRO genes,implying gene induction during cotton growth and development.Several miRNAs responsive to plant growth and abiotic stress were predicted to target 12 GhSRO genes.Organ-specific expression profiling demonstrated the roles of GhSRO genes in one or more tissues.In addition,specific expression pattern of some GhSRO genes dur-ing ovule development depicted their involvement in these developmental processes.Conclusion The data presented in this report laid a foundation for understanding the classification and functions of SRO genes in cotton. | SHABAN Muhammad TABASSUM Riaz RANA Iqrar Ahmad ATIF Rana Muhammad AZMAT Muhammad Abubakkar IQBAL Zubair MAJEED Sajid AZHAR Muhammad Tehseen | 2024 | Journal of Cotton Research2024，7，1： | 0 |
| 10 | A discussion on cotton transformation during the last decade (2010–2021);an update on present trends and future prospects显示文摘The introduction of genetically modified(GM)cotton in 1996 in the US and its worldwide spread later rejuvenated cotton production in many parts of the world.The evolution is continued since then and currently,the 3rd and fourth generation of same GM cotton is grown in many parts of the world.The GM cotton introduced in 1996 was simple Bt cotton that expressed a single Cry1Ac gene,the later generation carried multiple Cry genes along with the genes controlling herbicide tolerance.Current day GM cotton does not only give stable resistance against lepidopteran insects but also facilitates the farmers to spray broad-spectrum herbicides without harming the crop.The evolution of GM cotton is continued both on the basic and applied side and interventions have been introduced during the last decade.Earlier the cotton transformation was limited to Cocker strains which are getting possible in many other varieties,too.It is successful with both gene gun,and Agrobacterium and inplanta transformation has made it a routine activity.Apart from overexpression studies for various purposes including biotic,abiotic,and quality traits,RNAi and genome editing are explored vigorously.Through this review,we have tried to explore and discuss various interventions for improving transformation protocols,the applications of cotton transformation,and future strategies being developed to get maximum benefits from this technology during the last decade. | QANDEEL‑E‑ARSH AZHAR Muhammad Tehseen ATIF Rana Muhammad ISRAR Mahwish KHAN Azeem Iqbal KHALID Shahzad RANA Iqrar Ahmad | 2021 | Journal of Cotton Research2021，4，4： | 0 |
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