|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Comparative transcriptional analysis provides insights of possible molecular mechanisms of wing polyphenism induced by postnatal crowding in Aphis gossypii显示文摘Background:Aphis gossypii is a worldwide sap-sucking pest with a variety of hosts and a vector of more than 50 plant viruses.The strategy of wing polyphenism,mostly resulting from population density increasing,contributes to the evolutionary success of this pest.However,the related molecular basis remains unclear.Here,we identified the effects of postnatal crowding on wing morph determination in cotton aphid,and examined the transcriptomic differences between wingless and wing morphs.Results:Effect of postnatal crowding on wing determination in A gossypii was evaluated firstly.Under the density of 5 nymphs·cm-2,no wing aphids appeared.Proportion of wing morphs rised with the increase of density in a certain extent,and peaked to 56.1% at the density of 20 nymphs·cm-2,and reduced afterwards.Then,transcriptomes of wingless and wing morphs were assembled and annotated separately to identify potentially exclusively or differentially expressed transcripts between these two morphs,in which 3 126 and 3 392 unigenes annotated in Nr(Non-redundant protein sequence) database were found in wingless or wing morphs exclusively.Moreover,3 187 up-and 1 880 down-regulated genes were identified in wing versus wingless aphid.Pathways analysis suggested the involvement of differentially expressed genes in multiple cellular signaling pathways involved in wing morphs determination,including lipid catabolic and metabolism,insulin,ecdysone and juvenile hormone biosynthesis.The expression levels of related genes were validated by the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR) soon afterwards.Conclusions:The present study identified the effects of postnatal crowding on wing morphs induction and demonstrated that the critical population density for wing morphs formation in A gossypii was 20 nymphs·cm-2.Comparative transcriptome analysis provides transcripts potentially expressed exclusively in wingless or wing morph,respectively.Differentially expressed genes between wingless and wing morphs were identified and several signaling pathways potentially involved in cotton aphid wing differentiation were obtained. | JI Jichao ZHANG Shuai LUO Junyu WANG Li ZHU Xiangzhen ZHANG Kaixin ZHANG Lijuan CUI Jinjie | 2019 | Journal of Cotton Research2019，2，3： | 3 |
| 2 | Insights into wing dimorphism in worldwide agricultural pest and hostalternating aphid Aphis gossypii显示文摘Background:The worldwide pest Aphis gossypii has three-winged morphs in its life cycle,namely,winged parthenogenetic female(WPF),winged gynopara(GP),and winged male,which are all produced by a wingless parthenogenetic female(WLPF).Most studies on A.gossypii have focused on WPF,while few have investigated GP and male.The shared molecular mechanism underlying the wing differentiation in the three wing morphs of A.gossypii remains unknown.The wing differentiation of WPF was explored in a previous study.Herein,GP and male were induced indoors.The characters of the body,internal genitals,wing veins,and fecundity of GP and male were compared with those of WPF or WLPF.Compared with WLPF,the shared and separate differentially expressed genes(DEGs)were identified in these three-wing morphs.Results:Newly-born nymphs reared in short photoperiod condition(8 L:16D,18°C)exclusively produced gynoparae(GPe)and males in adulthood successively,in which the sex ratio was GP biased.A total of 14 GPe and 9 males were produced by one mother aphid.Compared with WLPF,the three-wing morphs exhibited similar morphology and wing vein patterns but were obviously discriminated in the length of fore-and underwings,reproductive system,and fecundity.A total of 37090 annotated unigenes were obtained from libraries constructed using the four morphs via RNA sequencing(RNA-Seq).In addition,10867 and 19334 DEGs were identified in the pairwise comparison of GP versus WLPF and male versus WLPF,respectively.Compared with WLPF,the winged morphs demonstrated 2335 shared DEGs(1658 upregulated and 677 downregulated).The 1658 shared upregulated DEGs were enriched in multiple signaling pathways,including insulin,FoxO,MAPK,starch and sucrose metabolism,fatty acid biosynthesis,and degradation,suggesting their key roles in the regulation of wing plasticity in the cotton aphid.Forty-four genes that spanned the range of differential expression were chosen to validate statistical analysis based on RNA-Seq through the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR).The comparison concurred with the expression pattern(either up-or downregulated)and supported the accuracy and reliability of RNA-Seq.Finally,the potential roles of DEGs related to the insulin signaling pathway in wing dimorphism were discussed in the cotton aphid.Conclusions:The present study established an efficiently standardized protocol for GP and male induction in cotton aphid by transferring newly-born nymphs to short photoperiod conditions(8 L:16D,18°C).The external morphological characters,especially wing vein patterns,were similar among WPFs,GPe,and males.However,their reproductive organs were strikingly different.Compared with WLPF,shared(2335)and exclusively(1470 in WLPF,2419 in GP,10774 male)expressed genes were identified in the three-wing morphs through RNA-Seq,and several signaling pathways that are potentially involved in their wing differentiation were obtained,including insulin signaling,starch and sucrose metabolism. | JI Jichao HUANGFU Ningbo LUO Junyu GAO Xueke NIU Lin ZHANG Shuai CUI Jinjie | 2021 | Journal of Cotton Research2021，4，1： | 2 |
| 3 | Effects of NaCl stress on the biochemical substances in Bt cotton as well as on the growth and development and adult oviposition selectivity of Helicoverpa armigera显示文摘Background:Recently,due to the development of food security strategies,cotton has been planted in inland saline-alkali dry soils or in coastal some saline-alkali soils in China.Under the condition,to comprehensively prevent and control Helicoverpa armigera in cotton fields with saline-alkali soils,it is important to study the larval growth and development of H.armigero and to study adult oviposition selectivity in H.armigera adults that feed on NaCI-stNaCled cotton plants.Results:In this study,Bt cotton GK19 was used for the experimental group and its nontransgenic parent Simian 3 was used for the control to study the effects of biochemical substances in cotton as well as larval growth and development and adult oviposition selectivity of H.armigera.The experiments were performed by growing cotton indoors under NaCl stress at concentrations of 0 mmol-L-1,75 mmol-L-1 and 150 mmol-L-1,respectively.The results showed that the expression of Bt protein was significantly inhibited for NaCI-stressed Bt cotNaClThe content of soluble protein and K+ in the leaves of cotton were decreased,while the content of gossypol and Na+were increased.In addition,the 5th instar H.armigera larvae exhibited shorten the life span in a 13-day trial period.Under enclosure treatments and at different female densities,the adult oviposition of H.armigera decreased on high NaCI-stressed nontransgenic coNaCl,while the oviposition on Bt cotton tended to first increase but then decrease under low,moderate and high NaCl stress treatments.Conclusions:Under certain content ranges of NaCl stress treatments,larval of H.armigera growth and development,and adult oviposition were no significant difference in the change for a certain period.However,under high NaCl stress,larval growth,development and adult oviposition were affected,which may provide insights for the prevention and control of H.armigero for Bt cotton in saline-alkali soils. | LUO Junyu ZHANG Shuai ZHU Xiangzhen JI Jichao ZHANG Kaixin WANG Chunyi ZHANG Lijuan WANG Li CUI Jinlie | 2019 | Journal of Cotton Research2019，2，1： | 1 |
| 4 | Lung epithelial branching program antagonizes alveolar differentiation显示文摘 | Daniel R. Chang Denise Martinez Alanis Rachel K. Miller Hong Ji Haruhiko Akiyama Pierre D. McCrea Jichao Chen | 2013 | Proceedings of the National Academy of Sciences2013，，45： | 1 |
| 5 | Dynamics and diversity of symbiotic bacteria in Apolygus lucorum at different developmental stages显示文摘Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic losses.Symbiotic bacteria living in insects play a key role in the nutrition,physiology,and behavior of hosts.Here,we present an experiment using Illumina HiSeq sequencing targeting the V3–V4 regions of bacteria’s 16S rRNA throughout the entire life cycle of A.lucorum.Results The first and second instar nymphs have the largest alpha diversity compared with other life stages of the insect.Bacterial phyla Proteobacteria(72.29%),Firmicutes(15.24%),Actinobacteria(7.76%)exhibit the largest relative abundance in all developmental stages.Erwinia(23.97%)and Lactococcus(10.62%)are the two genera with the high-est relative abundance.The relative abundance of Erwinia in the nymph stage is significantly greater than the adult stage,and the relative abundance of Lactococcus in 6-day-old and 9-day-old adult females is higher compared with adult males.Conclusions These results reveal that microbial community composition and relative abundance shift dynamically at different life stages,implying that different bacterial phyla and genera may have specific roles in specific life stages such as metabolism,nutrition absorption,detoxification,and reproduction.This study reveals for the first time the community composition and ecological dynamics of symbiotic bacteria throughout the life stages of A.lucorum,and thus may provide insight to new strategies for pest control. | XUE Hui ZHU Xiangzhen WANG Li ZHANG Kaixin LI Dongyang JI Jichao NIU Lin GAO Xueke LUO Junyu CUI Jinjie | 2023 | Journal of Cotton Research2023，6，1： | 0 |
| 6 | Comparative transcriptional analysis and identification of hub genes associated with wing differentiation of male in Aphis gossypii显示文摘Background:Aphis gossypii Glover(Hemiptera:Aphididae),a worldwide polyphagous phloem-feeding agricultural pest,has three wing morphs(winged parthenogenetic female,gynopara,and male)in the life cycle.The exclusive males could fly from summer hosts to winter hosts,which are essential for gene exchanges of cotton aphid populations from different hosts or regions.However,the molecular mechanism of wing differentiation of male in A.gossypii remains unclear.Results:Morphological observation of male A.gossypii showed that there is no distinct difference in the external morphologies of the 1st and 2nd instar nymphs.The obvious differentiation of wing buds started in the 3rd instar nymph and was visible via naked eyes in the 4th instar nymphal stage,then adult male emerged with full wings.According to morphological dynamic changes,the development of wings in males were divided into four stages:preliminary stage(the 1st instar to 2nd instar),prophase(the 3rd instar),metaphase(the 4th instar),anaphase(the 5th instar).Results of feeding behavior monitoring via EPG(electrical penetration graph)technology indicated that although the male cotton aphids had strong desire to feed(longer duration of C 55.24%,F 5.05%and Pd waves 2.56%),its feeding efficiency to summer host cotton was low(shorter E13.56%and E2 waves 2.63%).Dynamic transcriptome analysis of male aphid at 5 different developmental periods showed that in the 3rd instar nymph,the number of up-regulated DEGs was significant increased,and time-course gene transcriptional pattern analyses results also showed that numerous genes categorized in clusters 3,5,and 8 had the highest expressed levels,which were consistent with morphological changes of wing buds.These results indicate that the 3rd instar nymph is the critical stage of wing bud differentiation in males.Furthermore,through pathway enrichment analysis of DEGs and WGCNA,it revealed that the neuroactive ligand-receptor interaction,Ras signaling pathway,dopaminergic synapse,circadian entrainment and the corresponding hub genes of PLK1,BUB1,SMC2,TUBG,ASPM,the kinesin family members(KIF23,KIF20,KIF18-19)and the novel subfamily of serine/threonine(Aurora kinase A and Aurora kinase B)probably played an important role in the critical stage of wing bud differentiation.Conclusion:This study explored morphological changes and genes transcriptional dynamics males in cotton aphid,revealed the phenomenon of low feeding efficiency of winged males on summer host cotton,and identified key signaling pathways and potential hub genes potentially involved in wing bud differentiation of male in A.gossypii. | HUANGFU Ningbo SHI Qingyu CHEN Lulu MA Xiaoyan ZHANG Kaixin LI Dongyang WANG Li ZHU Xiangzhen JI Jichao LUO Junyu CUI Jinjie | 2022 | Journal of Cotton Research2022，5，3： | 0 |
| 7 | An overview of efficient development practices at low permeability sandstone reservoirs in China显示文摘Low permeability sandstone reservoirs in China typically have more complicated geological conditions, pore structures, and flow characteristics as compared to medium-to-high-permeability sandstone reservoirs. Traditional geological and seepage theories, and engineering methods are not applicable to the development of these low permeability reservoirs, and wells drilled into them often produce oil and gas at very low rates. Recent breakthroughs in reservoir exploitation technology have greatly improved the productivity of low permeability reservoirs, making them the primary target for oil exploration and extraction in China. The development theories and practices applied to low permeability reservoirs in China are reviewed in this study— based on relevant geological and engineering practices, including drilling, fracturing, recovery, and surface engineering. A unique series of technological advances that aid the development of low permeability reservoirs in China are summarized here. This study may serve as a meaningful guide in achieving scale efficiency for the development of low permeability reservoirs. | Bingyu Ji Jichao Fang | 2023 | Energy Geoscience2023，4，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员