| 1 | Specific targeted killing of ErbB2 positive breast cancer by retrovirus-mediated Immunocaspase-3 secreting T cells显示文摘In this study, lmmunocaspase-3 gene was transfected into Jurkat T lymphocytes and the targeted proapoptotic protein Immunocaspase-3 was stably secreted.Its entry to ErbB2 positive SKBr3 breast carcinoma cell line was observed by indirect immunofluorescence staining.Growth of SKBr3 cells was significantly inhibited when they were cultured with medium containing Immunocaspase-3.Next, lmmunocaspase-3 gene was cloned into retrovirus vector pLNCX, which was then transfected into PA317 cells to package. Packaged cells producing high titer pseudoviruses were acquired and the pseudoviruses were harvested to infect PBMCs, which had been stimulated to division. The latter were selected and administered to nude mice bearing SKBr3 tumors through tail vein. The results showed that the treatment contributed to an inhibition of tumor growth and prolonged the lifetime of nude mice bearing SKBr3 tumor.The efficiency of inhibition of tumor reached 73.25%, and the average lifetime of treated nude mice was 80.95% longerthan that of control group. Immunohistochemical examination revealed the exclusive distribution of Immunocaspase-3 proteins only in the tumor tissue samples; and TUNEL assay confirmed the occurrence of apoptosis in tumor calls. Thepresent study suggests that Immunocaspase-3 secreted by T lymphocytes can selectively bind and enter into ErbB2 positive breast cancer cells, where it exhibits a proapoptotic activity and causes tumor suppression in an in vivo tumor model. | ZHANGLihong JIALintao ZHAOJing XUYanming WENWeihong BAOWei CAOYunxin SUChenazhi WANGChengji YANGAn-gang | 2004 | Chinese Science Bulletin2004，49，13： | 5 |
| 2 | Adaptation of Cu/ZnO/Al_2O_3 to Temperature Change in Methanol Synthesis from CO_2 Hydrogenation显示文摘The induction behavior in CO2 hydrogenation was studied by varying the reaction temperature to investigate the adaptation of the Cu/ZnO/Al2O3 catalyst to the temperature change,The results indicated that a used catalyst had a tendency to keep the last running state in new reaction conditions for MeOH formation,and that this tendency was related to the difference in Cu/Cu^n+ ration caused by CO2 and CO produced at different reaction temperatures,However,the reverse water-gas shift reaction (BWGS) induced at four temperatures was completely different from that of methanol synthesis,It implied that the two so-called competitive reactions in CO2+H2,RWGS and methanol synthesis,have different, active centers. | JialinTao Ki-WonJun 等 | 2002 | Journal of Natural Gas Chemistry2002，11，1： | 0 |
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