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| 1 | Spatial pattern of grassland aboveground biomass and its environmental controls in the Eurasian steppe显示文摘Vegetation biomass is an important component of terrestrial ecosystem carbon stocks. Grasslands are one of the most widespread biomes worldwideplaying an important role in global carbon cycling. Thereforestudying spatial patterns of biomass and their correlations to environment in grasslands is fundamental to quantifying terrestrial carbon budgets. The Eurasian steppean important part of global grasslandsis the largest and relatively well preserved grassland in the world. In this studywe analyzed the spatial pattern of aboveground biomass(AGB)and correlations of AGB to its environment in the Eurasian steppe by meta-analysis. AGB data used in this study were derived from the harvesting method and were obtained from three data sources(literatureglobal NPP database at the Oak Ridge National Laboratory Distributed Active Archive Center(ORNL)some data provided by other researchers). Our results demonstrated that:(1) as for the Eurasian steppe overallthe spatial variation in AGB exhibited significant horizontal and vertical zonality. In detailAGB showed an inverted parabola curve with the latitude and with the elevationwhile a parabola curve with the longitude. In additionthe spatial pattern of AGB had marked horizontal zonality in the Black Sea-Kazakhstan steppe subregion and the Mongolian Plateau steppe subregionwhile horizontal and vertical zonality in the Tibetan Plateau alpine steppe subregion.(2) Of the examined environmental variablesthe spatial variation of AGB was related to mean annual precipitation(MAP)mean annual temperature(MAT)mean annual solar radiation(MAR)soil Gravel contentsoil p H and soil organic content(SOC) at the depth of 0–30 cm. NeverthelessMAP dominated spatial patterns of AGB in the Eurasian steppe and its three subregions.(3) A Gaussian function was found between AGB and MAP in the Eurasian steppe overallwhich was primarily determined by unique patterns of grasslands and environment in the Tibetan Plateau. AGB was significantly positively related to MAP in the Black Sea-Kazakhstan steppe subregion(elevation < 3000 m)the Mongolian Plateau steppe subregion(elevation < 3000 m) and the surface(elevation ≥ 4800 m) of the Tibetan Plateau. Neverthelessthe spatial variation in AGB exhibited a Gaussian function curve with the increasing MAP in the east and southeast margins(elevation < 4800 m) of the Tibetan Plateau. This study provided more knowledge of spatial patterns of AGB and their environmental controls in grasslands than previous studies only conducted in local regions like the Inner Mongolian temperate grasslandthe Tibetan Plateau alpine grasslandetc. | JIAO Cuicui YU Guirui HE Nianpeng MA Anna GE Jianping HU Zhongmin | 2017 | Journal of Geographical Sciences2017，27，1： | 7 |
| 2 | Quantitative Simulation on Soil Moisture Contents of Two Typical Vegetation Communities in Sanjiang Plain,China显示文摘Different types of vegetation occupy different geomorphology and water gradient environments in the San-jiang Plain,indicating that the soil moisture dynamics and water balance patterns of the different vegetation communi-ties might differ from each other.In this paper,a lowland system,perpendicular to the Nongjiang River in the Honghe National Nature Reserve(HNNR),was selected as the study area.The area was occupied by the non-wetland plant forest and the typical wetland plant meadow.The Microsoft Windows-based finite element analysis software package for simulating water,heat,and solute transport in variably saturated porous media(HYDRUS),which can quantita-tively simulate water,heat,and/or solute movement in variably-saturated porous media,was used to simulate soil moisture dynamics in the root zone(20-40 cm) of those two plant communities during the growing season in 2005.The simulation results for soil moisture were in a good agreement with measured data,with the coefficient of determi-nation(R2) of 0.44-0.69 and root mean square error(RMSE) ranging between 0.0291 cm3/cm3 and 0.0457 cm3/cm3,and index of agreement(d) being from 0.612 to 0.968.During the study period,the volumetric soil moisture content of meadow increased with the depth and its coefficient of variation decreased with the depth(from 20 cm to 40 cm),while under the forest the soil moisture content at different depths varied irregularly.The calculated result of water budget showed that the water budget deficit of the meadow was higher than that of the forest,suggesting that the meadow is more likely to suffer from water stress than the forest.The quantitative simulation by HYDRUS in this study did not take surface runoff and plant growth processes into account.Improved root water uptake and surface runoff models will be needed for higher accuracy in further researches. | LI Shanghua ZHOU Demin LUAN Zhaoqing PAN Yun JIAO Cuicui | 2011 | Chinese Geographical Science2011，21，6： | 7 |
| 3 | Scale Issues of Wetland Classification and Mapping Using Remote Sensing Images: A Case of Honghe National Nature Reserve in Sanjiang Plain, Northeast China显示文摘Wetland research has become a hot spot linking multiple disciplines presently. Wetland classification and mapping is the basis for wetland research. It is difficult to generate wetland data sets using traditional methods because of the low accessibility of wetlands, hence remote sensing data have become one of the primary data sources in wetland research. This paper presents a case study conducted at the core area of Honghe National Nature Reserve in the Sanjiang Plain, Northeast China. In this study, three images generated by airship, from Thematic Mapper and from SPOT 5 were selected to produce wetland maps at three different wetland landscape levels. After assessing classification accuracies of the three maps, we compared the different wetland mapping results of 11 plant communities to the airship image, 6 plant ecotypes to the TM image and 9 landscape classifications to the SPOT 5 image. We discussed the different characteristics of the hierarchical ecosystem classifications based on the spatial scales of the different images. The results indicate that spatial scales of remote sensing data have an important link to the hierarchies of wetland plant ecosystems displayed on the wetland landscape maps. The richness of wetland landscape information derived from an image closely relates to its spatial resolution. This study can enrich the ecological classification methods and mapping techniques dealing with the spatial scales of different remote sensing images. With a better understanding of classification accuracies in mapping wetlands by using different scales of remote sensing data, we can make an appropriate approach for dealing with the scale issue of remote sensing images. | GONG Huili | 2011 | Chinese Geographical Science2011，21，2： | 5 |
| 4 | Structural insights into SUN-KASH complexes across the nuclear envelope显示文摘nucleoskeleton 和细胞骨架(LINC ) 的连接器建筑群由阳光和 KASH 包含域的蛋白质组成并且衔接原子信封的内部、外部的膜。LINC 建筑群在原子放,房间极化和细胞的僵硬起关键作用。以前,我们报导了人的 SUN2 的 homotrimeric 结构。我们现在决定了人的 SUN2-KASH 建筑群的水晶结构。在复杂结构, SUN 领域 homotrimer 绑在三独立 “ hook”相似 KASH 肽。在建筑群的 SUN 领域的全面符合构造非常处于它的 apo 状态类似于 SUN 领域。一个主要 conformational 变化包含 KASH 固定的阳光领域的 AA'-loop,它重排形成微型 β与 KASH 肽交往的表。KASH 领域的磅主题在 SUN 领域的 homotrimeric 接口上紧适合进一个恐水病的衣袋,我们它称为双性人衣袋。而且, SUN 领域 homotrimer 三明治的二邻近的 protomers 由结合的恐水病的相互作用和氢的 KASH 领域。这些有约束力的地点的变化在 vitro 破坏或减少在 SUN 和 KASH 域之间的协会。另外, transfection 野类型,然而并非变异, SUN2 在 Ovcar-3 房间支持房间移植。这些结果提供 LINC 建筑群的一个结构的模型,它为另外的学习是必要的物理、功能在细胞质和核质之间联合。 | Wenjia Wang Zhubing Shi Shi Jiao Cuicui Chen Huizhen Wang Guoguang Liu Qiang Wang Yun Zhao Mark I Greene Zhaocai Zhou | 2012 | Cell Research2012，22，10： | 3 |
| 5 | Genetically engineered bacterial-like particles induced specific cellular and humoral immunity as effective tick-borne encephalitis virus vaccine显示文摘Tick-borne encephalitis(TBE)is a natural focal disease with fatal encephalitis induced by tick-borne encephalitis virus(TBEV),seriously threatening human and public health.Protection of TBE depends on vaccination with inactivated vaccine,which requires high cost and multiple immunizations.Here,we construct genetically engineered bacterial-like particles(BLPs)as an effective TBEV vaccine with simplified immunizations and improved immune efficacy.The TBEV BLPs involve the combination of the gram-positive enhancer matrix from Lactococcus lactis,and TBEV envelope(E)protein expressed by genetically engineered recombinant baculovirus.The prepared TBEV BLPs can effectively stimulate the activation of dendritic cells to present the TBEV E proteins to T and B cells,leading to strong and durable cellular and humoral immune responses in mice.Surprisingly,the serum levels of specific IgG antibodies in mice remain about 10^(6)at 6 months after the secondary immunization.Overall,the TBEV BLPs can be used as a potent vaccine candidate,laying the foundation for developing novel TBEV genetically engineered vaccines. | Mengyao Zhang Hongli Jin Yuanyuan Li Cuicui Jiao Pei Huang Yujie Bai Zhiyuan Gong Haili Zhang Shunjie Liu Hualei Wang | 2023 | Aggregate2023，4，3： | 1 |
| 6 | Expression and Regulatory Network Analysis of Function of Small Nucleolar RNA Host Gene 4 in Hepatocellular Carcinoma显示文摘Background and Aims:Long non-coding RNA small nucleolar RNA host genes(SNHGs)play a critical role in the occurrence and development of tumors.In this study,we aimed to investigate the role of SNHG4 in hepatocellular carcinoma(HCC)and its underlining mechanism.Methods:Datasets were acquired from The Cancer Genome Atlas(TCGA)database.lncLocator 2.0 was used to identify the distribution of SNHG4 in HCC cells.Gene expression,Kaplan-Meier survival,microRNA and transcription factor target analyses were performed with the University of Alabama Cancer(UALCAN)Database,Kaplan-Meier Plotter,LinkedOmics,WebGestalt and gene set enrichment analysis,respectively.Gene Ontology and pathway enrichment analyses and assessment of RNA binding proteins were performed by R software,circlncRNAnet and Encyclopedia of RNA Interactomes(EN-CORI).In addition,CirclncRNAnet and ENCORI were used to find the correlation between SNHG4 and important proteins,while the prognostic value was assessed with the Human Protein Atlas database and Kaplan-Meier Plotter.Results:Expression of SNHG4 in HCC is higher in HCC tissue than in normal healthy liver tissues and is mainly distributed in the nucleus.SNHG4 positively correlated with poor prognosis(p<0.01 for overall survival and recurrence-free survival).Functional enrichment analysis revealed SNHG4 involve-ment with regulation of ribosomal RNA synthesis and the RNA processing and surveillance pathway.SNHG4 is closely associated with miR-154 and miR-206,transcription factor target E2F family and the signaling pathway for MAPK/ERK and mTOR.U2 auxiliary factor 2(U2AF2)showed strong correlation with SNHG4,while low-expression of U2AF2 showed good prognosis.Conclusions:Based on our find-ings,we infer SNHG4 may play a role in the formation of HCC via regulation of tumor-related pathways. | Jing Cao Cuicui Xiao Christ-Jonathan Tsia Hin Fong Jiao Gong Danyang Li Xiangyong Li Yusheng Jie Yutian Chong | 2022 | Journal of Clinical and Translational Hepatology2022，10，2： | 1 |
| 7 | Incorporation of Escherichia coli heat-labile enterotoxin B subunit into rabies virus particles enhances its immunogenicity in mice and dogs显示文摘Although inactivated vaccines against rabies have the advantage of high safety,effective protection against rabies virus(RABV)infection often requires multiple,high-dose immunization.Incorporating a molecular adjuvant into the viral particles has been found to be a useful strategy to promote the immune effectiveness of inactivated vaccines.In this study,we constructed a recombinant virus,rCVS11-LTB,which chimerically expresses a molecular adjuvant heat-labile enterotoxin B subunit(LTB)protein on the surface of the RABV particles.Immunogenicity in vivo was found to be promoted by rCVS11-LTB through the activation of dendritic cells(DCs).Our results demonstrated that inactivated rCVS11-LTB was able to induce higher levels of virusneutralizing antibodies(VNAs)in both mice and dogs than the parent virus rCVS11,to enhance the cellular immune response and T cell immune memory in mice,and was also able to provide 100%protection in mice from lethal doses of rabies virus,indicating its potential as a safe and effective inactivated rabies vaccine candidate. | Zhiyuan Gong Hailun Li Meichen Qian Yujie Bai Hongli Jin Jingxuan Sun Mengyao Zhang Cuicui Jiao Pei Huang Yuanyuan Li Haili Zhang Hualei Wang | 2023 | Biosafety and Health2023，5，5： | 0 |
| 8 | An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV显示文摘Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and animals.There is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in animals.Rabies virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health threat.The present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and dogs.The results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in mice.Notably,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer periods.Inactivated SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals. | Haili Zhang Hongli Jin Feihu Yan Yumeng Song Jiaxin Dai Cuicui Jiao Yujie Bai Jingxuan Sun Di Liu Shen Wang Mengyao Zhang Jilong Lu Jingbo Huang Pei Huang Yuanyuan Li Xianzhu Xia Hualei Wang | 2023 | Virologica Sinica2023，38，2： | 0 |
| 9 | The Application of a Safe Neutralization Assay for Ebola Virus Using Lentivirus-Based Pseudotyped Virus显示文摘Dear Editor,Zaire Ebola virus(EBOV)is a negative-sense singlestranded RNA virus that is capable of causing acute hemorrhagic fever with a frightening fatality rate that can reach up to 90%.Due to its high lethality and frequent recurrence,EBOV is a substantial threat to public health.Research about live EBOV is restricted to BSL-4 laboratories;therefore,conventional serological detection methods do not provide a safe way to evaluate neutralizing activity,resulting in a bottleneck in antibody-based detection of viruses such as EBOV.Here,we aim to develop and apply a neutralization assay for EBOV using a generated lentivirus-based pseudotyped EBOV bearing GP on its surface. | Zengguo Cao Hongli Jin Gary Wong Ying Zhang Cuicui Jiao Na Feng Fangfang Wu Shengnan Xu Hang Chi Yongkun Zhao Tiecheng Wang Weiyang Sun Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang | 2021 | Virologica Sinica2021，36，6： | 0 |
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