|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Regression of primary gastric B-cell mucosa-associated lymphoid tissue (MALT) lymphoma after eradication of Helicobacter pylori显示文摘 | Falco Jover G Martinez EgeaA Sanchez Cuenca J | 1999 | Rev Esp Enferm Dig1999，91，8： | 1 |
| 3 | Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profile显示文摘 | Moya M Gómez-Lechón MJ Castell JV Jover R | 2010 | Chem Biol Interact2010，184，3： | 1 |
| 4 | Plant microRNAs and devdopment显示文摘 | JOVER G CANDELA H PONCE M R | 2005 | Int J Dev Biol2005，49，56： | 1 |
| 5 | Down-regulation of human CYP3A4 by the inflammatory signal interleukin 6: molecular mechanism and transcription factors involved 显示文摘 | Jover R Bort R Gómez-Lechón MJ | 2002 | FASEB J2002，16，13： | 1 |
| 6 | Additional work of breathing and breathing patterns in spontaneously breathing patients during pressure support ventilation, automatic tube compensation and amplified spontaneous pattern breathing显示文摘 | Aguilar G Jover JL Soro JM | 2005 | Eur J Anaesthesiol2005，22，4： | 1 |
| 7 | Potentiation of heroin and methadone hepatotoxicity by ethanol: an in vitro study using cultured human hepatocytes显示文摘 | R. Jover X. Ponsoda M. J. Gómez-lechón J. V. Castell | 1992 | Xenobiotica1992，，： | 1 |
| 8 | Transcriptional regulation of cytochrome p450 genes by the nuclear receptor hepatocyte nuclear factor 4-α显示文摘 | Jover R Moya M Gómez-Lechón M J | 2009 | Curr Drug Metab2009，10，5： | 1 |
| 9 | Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profile on nonalcoholic fatty liver disease显示文摘 | Moya M Gmez-Lechón MJ Castell JV Jover R | 2010 | Chem Biol Interact2010，184，3： | 1 |
| 10 | Laparoscopic cholecystectomy and incidental carcinoma of extrahepatic bilary tree 显示文摘 | Silecchia G Raparelli L Jover navalon JM | 2002 | JSLS2002，6，4： | 1 |
| 11 | Growth of mediterra-nean yellowtail(Seriola dumerilii)fed extruded diets con-taining different levels of protein and lipid显示文摘 | Jover M Garcia G A Tomas A | 1999 | Aquaculture1999，179，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员