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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Decreased bone speed of sound in children with growing pains measured by quantitative ultrasound显示文摘 | Hashkes PJ Jaber L | 2005 | J Rheumatol2005，32，7： | 1 |
| 3 | Decreased pain threshold in children with growing pains 显示文摘 | Haskes PJ Friedland O Jaber L | 2004 | Rheumatol2004，31，3： | 1 |
| 4 | A new mutation in the growth hormone - releasing hormone receptor gene in two Israeli Arab families 显示文摘 | Haskin O Lazar L Jaber L | 2006 | J Endocrinot Invest2006，29，2： | 1 |
| 5 | Impact of short daily hemodialysis on restless legs symptoms and sleep disturbances显示文摘 | Jaber B L Schiller B Burkart J M | 2011 | Clin J Am Soc Nephrol2011，6，： | 1 |
| 6 | Hemodialysis in acute renal failure: Does the membrane matter? 显示文摘 | MODI G K PEREIRA B J G JABER B L | 2001 | Seminars in Dialysis2001，14，5： | 1 |
| 7 | Dose of erythropoiesis-stimulating agents and adverse outcomes in CKD: A Metaregression Analysis显示文摘 | Koulouridis I Alfayez M Trikalinos T A Balk E M Jaber B L | 2013 | Am J Kidney Dis2013，61，： | 1 |
| 8 | Pulmonary infectious mortality amongpatientswith end-stage renal disease显示文摘 | SAMAKM J JABER B L | 2001 | Chest2001，120，6： | 1 |
| 9 | A joint model for cashand inventory management for a retailer under delay inpayments显示文摘 | Moussawi-Haidar L Jaber M Y | 2013 | Computers & Industrial Engineering2013，66，4： | 1 |
| 10 | Mortality caused by sepsis m pa- tients with end-stage renal disease compared with the general population显示文摘 | SARNAK M J JABER B L | 2000 | Kidney Int2000，58，4： | 1 |
| 11 | Daily hemofiltration for end-stage renal disease:a feasibility and efficacy trial显示文摘 | JABER B L ZIMMERMAN D L TEEHAN G S | 2004 | Blood Purif2004，22，3： | 1 |
| 12 | Pulmonary infections mortality amongpatients with end stage renal disease显示文摘 | Eli Sarnak M J Jaber B L | 2001 | Chest2001，120，6： | 1 |
| 13 | Incidence and prevalence of hyponatremia 显示文摘 | UPADHYAY A JABER B L MADIAS N E | 2006 | Am J Med2006，119，71： | 1 |
| 14 | Sensorineural deafness in-herited as a tissue specific mitocbondrial disorder显示文摘 | Jaber L Shohat M Bu X | 1992 | JMed Genet1992，29，2： | 1 |
| 15 | Sensorineural deafness inherited as a tissue specific mitocbondrial disorder显示文摘 | Jaber L Shohat M Bu X | 1992 | J Med Genet1992，29，2： | 1 |
| 16 | Non - identical distribution pattern of epidermal growth factor and platelet - derived growth factor in the mouse uterus during the oestrous cycle and early pregnancy 显示文摘 | JABER L KAN F W | 1998 | Histochem J1998，30，10： | 1 |
| 17 | Extended Firewall for Re- gression Testing:an Experience Report 显示文摘 | White L Jaber K Robinson B | 2008 | Journal of Software Maintenance and Evolution2008，20，6： | 1 |
| 18 | C - reactive protein levels and outcomes after statin therapy显示文摘 | JABER B L MADIAS N E | 2005 | N Engl J Med2005，352，1： | 1 |
| 19 | Incidence and prevalence of hy- ponatremia 显示文摘 | UPADHYAY A JABER B L | 2009 | The American Journal of Medicine2009，1，7： | 1 |
| 20 | Fluid management and risk factors for renal dysfunction in patients with severe sepsis and/or septic shock显示文摘 | Muller L Jaber S Molinari N | 2012 | Crit Care2012，16，： | 1 |
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