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| 1 | Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC. | Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel | 2017 | World Journal of Stem Cells2017，9，9： | 4 |
| 2 | Product and environmental social performance: Varying effect on firm performance显示文摘 | Satish Jayachandran Kartik Kalaignanam Meike Eilert | 2013 | Strat. Mgmt. J2013，，10： | 3 |
| 3 | Customer response capability in a sense-and-respond era: The role of customer knowledge process显示文摘 | Satish Jayachandran Kelly Hewett Peter Kaufman | 2004 | Journal of the Academy of Marketing Science2004，，3： | 2 |
| 4 | Synthesis and characterization of copper substituted lithium manganate spinels 显示文摘 | MURALI K R SARAVANAN T JAYACHANDRAN M | 2008 | Mater Electron2008，19，： | 1 |
| 5 | Development of a Novel High Optical Quality ZnO Thin Films by PLD for III-V Optoelectronic Devices 显示文摘 | Ramamoorthy K Sanjeeviraja G Jayachandran M | 2006 | Current Applied Physics2006，6，1： | 1 |
| 6 | Transgelin is a direct target of TGF-beta/ Smad3-dependent epithelial cell mi- gration in lung fibrosis 显示文摘 | Yu H Konigshoff M Jayachandran A | 2008 | FASEB J2008，，22： | 1 |
| 7 | Induction of apoptosis and cell cycle arrest by Bis (2 ethylhexyl) phthalate produced by marine Bacilluspumilus MB40 显示文摘 | Moushumi Priya A Jayachandran S | 2012 | ChemBiolInteract2012，195，2： | 1 |
| 8 | Estrogen prevents destabiluzation of endothelial nitric oxide synthase mRNA induced by tumor necrosis factor alpha through estrogen receptor mediated system 显示文摘 | Sumi D Hayashi T Jayachandran M | 2001 | Life Sci2001，69，14： | 1 |
| 9 | Role of the Na+/H+ exchanger in short-term atrial electrophysiological remodeling显示文摘 | Jayachandran JV Zipes DP Weksler J | 2000 | Circulation2000，101，： | 1 |
| 10 | Diabetes and outcomes after radical prostatectomy:are results affected by obesity and race?Results from the shared equal-access regional cancer hospital database显示文摘 | Jayachandran J Aronson W Terris M | 2010 | Cancer Epidemiol Biomarkers Prev2010，1，19： | 1 |
| 11 | Market orientation: a meta- analytic review and assessment of its antecedents and impact on performance 显示文摘 | Kirca Jayachandran Bearden | 2005 | Journal of Marketing2005，69，2： | 1 |
| 12 | Phenotypic and genetic diversity of Bacillus thuringiensis strains isolated in India active against Spodoptera litura显示文摘 | ORABAGARAN S R NIMAL S J JAYACHANDRAN S | 2002 | Appl Biochem Biotechnol2002，，16： | 1 |
| 13 | BRAF - mutated,, microsatellite - stable adenocarcinoma of the proximal colon : an aggressive adenocarcinoma with poor survival, mucinous differentiation, and adverse morphologic features 显示文摘 | Pai R K Jayachandran P Koong A C | 2012 | Test Site2012，36，5： | 1 |
| 14 | Preliminary characterization of exopolysaccharides produced by a marine biofilmforming bacterium Pseudoalteromonas ruthenica (SBT 033 ) 显示文摘 | SARAVANAN P JAYACHANDRAN S | 2008 | Letters in Applied Microbiology2008，46，01： | 1 |
| 15 | Cathodic electrodeposition and analysis of SnS films for photoelectrochemical cells显示文摘 | Subramanian B Sanjeeviraja C Jayachandran M | 2001 | Materials Chemistry and Physics2001，71，： | 1 |
| 16 | Role of the Na^+/H^+ exchanger in short-term atrial electrophysiological remodeling 显示文摘 | Jayachandran JV Zipes DP Weksler J | 2000 | Circulation2000，101，15： | 1 |
| 17 | Theory of Multimarket Competition:a Synthesis and Implications for Marketing Strategy显示文摘 | Jayachandran S Gimeno J Varadarajan P.R | | 0，，： | 1 |
| 18 | Panickar,intramuscular triamcinolone for difficult asthma显示文摘 | Jayachandran R | 2005 | Pediatric Pulmonology2005，39，3： | 1 |
| 19 | Experimental studies on composite deck slabs to determine the shear-bond characteristic (m-k)values of the embossed profiled sheet显示文摘 | MARIMUTHU V SEETHARAMAN S ARUL JAYACHANDRAN S | 2007 | Contructional Steel Research2007，63，： | 1 |
| 20 | Pharma- cokinetic data on doxycycline and its distribution in different biological fluids in female goats 显示文摘 | Jha V K Jayachandran C Sinagh M K | 1989 | Veterinary Research Communications1989，，13： | 1 |
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