| 1 | Implication of snoRNA U50 in human breast cancer显示文摘Deletion of chromosome 6q is frequent in breast cancer,and the deletion often involves a region in 6q14-q16.At present,however,the underlying tumor suppressor gene has not been established.Based on a recent study identifying snoRNA U50 as a candidate for the 6q14-16 tumor suppressor gene in prostate cancer,we investigated whether U50 is also involved in breast cancer.PCR-based approaches showed that U50 underwent frequent genomic deletion and transcriptional downregulation in cell lines derived from breast cancer.Muta-tion screening identified the same 2-bp deletion of U50 as in prostate cancer in both cell lines and primary tumors from breast cancer,and the deletion was both somatic and in germline.Genotyping of a cohort of breast cancer cases and controls for the mutation demonstrated that,while homozygous genotype of the mutation was rare,its heterozygous genotype occurred more frequently in women with breast cancer.Functionally,re-expression of U50 resulted in the inhibition of colony formation in breast cancer cell lines.These results suggest that noncoding snoRNA U50 plays a role in the development and/or progression of breast cancer. | Xue-Yuan Dong Peng Guo Jeff Boyd Xiaodong Sun Qunna Li Wei Zhou Jin-Tang Dong | 2009 | Journal of Genetics and Genomics2009，36，8： | 11 |
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