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| 1 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Isolation and identification otriester-diterpenoid from the flowers ofAconitum kusnezoffiReichb显示文摘 | Huang ZH Jia SS | 1999 | Acta Pharm Sin1999，34，11： | 1 |
| 3 | Allolymphocyte apoptosis induced by soluble liver specific antigen 显示文摘 | Jia CK Wang Y Zheng SS | 2003 | Hepatobiliary Pancreat Dis Iht2003，2，2： | 1 |
| 4 | Down-regulation of hypoxia inducible fac- tor-la: a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance 显示文摘 | Jia SS Liu YH | 2010 | Eur J Oral Sci2010，118，2： | 1 |
| 5 | Down-regulation of hypoxia inducible factor-lalpha: a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance 显示文摘 | Jia SS Liu YH | 2010 | Eur J Oral Sci2010，118，2： | 1 |
| 6 | 17 beta-Estradiol accentuates contractility of rat genioglossal muscle via regulation of estrogen receptor alpha显示文摘 | Hou YX Jia SS Liu YH | 2010 | Arch Oral Biol2010，55，4： | 1 |
| 7 | Coordination compounds in nuclear medicine 显示文摘 | Jurisson SS Berning D Jia W | 1993 | Chem Rev1993，93，3： | 1 |
| 8 | Effects of ovariectomy on rat genioglossal muscle contractile properties and fiber-type distribution 显示文摘 | Liu YH Jia SS Hou YX | 2009 | Angle Orthod2009，79，3： | 1 |
| 9 | Seroprevalence of humanpapillomavirus types 6,11,16 and 18 in Chinese women显示文摘 | Jia J Jennifer SS He Wang et a I | 2012 | BMC InfectDis2012，12，1： | 1 |
| 10 | Treatment of maxillary sinus carcinoma: a preliminary evaluation显示文摘 | Jia SS | 1990 | Proceedings of the Fourth National Congress on Oncology1990，，： | 1 |
| 11 | Down-regulation of hypoxia inducible factor1 alpha:a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance显示文摘 | Jia SS Liu YH | 2010 | Eur J Oral Sci2010，118，2： | 1 |
| 12 | Down-regulation of hypoxia inducible factor-1alpha:a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance显示文摘 | Jia SS Liu YH | 2010 | Eur J Oral Sci2010，118，2： | 1 |
| 13 | Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cell显示文摘 | Jia SS Xi GP Zhang M | 2013 | Oncol Rep2013，29，1： | 1 |
| 14 | Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression显示文摘 | Li SS Wen JP Jia XP | 2011 | Applied Microbiology and Bioteehnology2011，91，3： | 1 |
| 15 | An improved on-line solid phase extraction coupled HPLC-MS/MS system for quantification of Sifuvirtide in human plasma 显示文摘 | Wang QQ Xiang SS Jia YB | 2010 | J ChromatogrB2010，878，21： | 1 |
| 16 | Acrolein,a toxicant in cigarette smoke,causes oxidative damage and mitochondrial dysfunction in RPE cells:protection by (R)-alpha-lipoic acid显示文摘 | Jia L Liu Z Sun L Miller SS Ames BN Cotman CW | | 0，，01： | 1 |
| 17 | Vertebrate heart growth is regulatedby functional antagonism between Gridlock and Gata5 显示文摘 | Jia H King IN Chopra SS | 2007 | Proc NatlAcad Sci U S A2007，104，14： | 1 |
| 18 | Increased expression of chondroitin sulphale proteoglycans in rat hepatocellular carcinoma tissues 显示文摘 | Jia XL Li SY Dang SS | 2012 | World J Gastroenterol2012，18，30： | 1 |
| 19 | A new silymarin preparation based on solid dispersion technique显示文摘 | Qiu MF Jia W Li SS | 2005 | Adv Ther2005，22，6： | 1 |
| 20 | The role of endothelin-1 in Binswanger,s disease显示文摘 | Chen CF Guo SS Jia HY | 1996 | Zhong hua yao li xue za zhi1996，58，3： | 1 |
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