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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Overexpression of cyctin D1 enhences gene amplification 显示文摘 | Zhou P Jiang W Weghorst CM | 1996 | Cancer Res1996，56，1： | 1 |
| 3 | Two-phase clustering process for outliers detection显示文摘 | JIANG MF TSENG SS SU CM | 2001 | Computational Statistics and Data Analysis2001，36，3： | 1 |
| 4 | Rapid cytochrome c re- lease, activation of caspase 3, 6,7 and 8 followed by Bap31 cleav- age in HeLa cells treated with photodynamic therapy 显示文摘 | Granville DJ Carthy CM Jiang H | 1998 | Febs Lett1998，437，12： | 1 |
| 5 | The immune factors involved in the pathogenesis, diagnosis, and treatment of Sjogren's syndrome 显示文摘 | Huang YF Cheng Q Jiang CM | 2013 | Clin Dev Immunol2013，2013，16： | 1 |
| 6 | Are current defimtiolts of sarcopenia applicable for older Chinese adults? 显示文摘 | Wen X Wang M Jiang CM etal | 2011 | J Nutr Hea- lthAging2011，15，10： | 1 |
| 7 | Safety and effi- cacy of sertraline for depression in patients with heart failure: re- suits of the SADHART-CHF (Sertraline Against Depression and Heart Disease in Chronic Heart Failure) trial显示文摘 | O'connor CM Jiang Wei Kuchibhatla M | 2010 | J Am Coil Cardi- ol2010，56，9： | 1 |
| 8 | Comparative genomesequencing for discovery of novel polymorphisms in Bacillus anthracis 显示文摘 | Read TD Salzberg SL Pop M Shumway M Umayam L Jiang L Holtzapple E Busch JD Smith KL Schupp JM Solomon D Keim P Fraser CM | 2002 | Science2002，296，5575： | 1 |
| 9 | Somatostatin receptors SSTR2 and SSTR5 are expressed in the human thoracic: duct 显示文摘 | Jiang H Deng XF Duan CM | 2011 | Lymphology2011，44，1： | 1 |
| 10 | System approach to structural topology optimization:designing optimal connections显示文摘 | Jiang T CM A | | 0，，12： | 1 |
| 11 | Observation of the nitrogen deposition in the lower Liaohe River Plain,Northeast China and assessing its ecological risk显示文摘 | Yu WT Jiang CM Ma Q | | 0，，： | 1 |
| 12 | A linear endothelin-1 an- alogue: solution structure of ET-1 by nuclear magnet ic resonance spectroscopy and molecular modelling 显示文摘 | Hewage CM Jiang L Parkinson JA | 1999 | Neurochem Int1999，35，1： | 1 |
| 13 | Caloric restriction and physical activity in zebrafish(Danio rerio)显示文摘 | Novak CM Jiang X Wang C | | 0，，： | 1 |
| 14 | Syntrophic growth on formate: a new microbial niche in anoxic environments 显示文摘 | Dolfing J Jiang B Henstra AM Stams AJ Plugge CM | 2008 | Appl Environ Microbiol2008，74，19： | 1 |
| 15 | Opposing effects of engagement of integrins and stimulation of cytokine receptors on cell cycle progression of normal human hematopoietic progenitors显示文摘 | Jiang YH Prosper F Verfaillie CM | 2000 | Blood2000，95，： | 1 |
| 16 | Stochastic state transitions give rise to phenotypic equilibrium in populations of cancer cells显示文摘 | Gupta PB Fillmore CM Jiang G | 2011 | Cell2011，146，4： | 1 |
| 17 | The associa- tion between depression and chronic kidney disease and mortality among patients hospitalized with congestive heart failure显示文摘 | Hedayati SS Jiang W O'Connor CM | 2004 | Am J Kidney Dis2004，44，： | 1 |
| 18 | Efect of dopamine receptor-1 on apoptosis of cultured neonatal rat eardiomyocytes in simulated ischemia reperfusion显示文摘 | Li HZ Han LP Jiang CM | 2008 | Basic Clini Pharmaeol Toxicol2008，102，3： | 1 |
| 19 | Many paths to alopeeia via compro- mised regeneration of hair follicle stem cells 显示文摘 | Li J Jiang TX Chuong CM | 2013 | J Invest Derma- tol2013，133，6： | 1 |
| 20 | Dimensions of acculturation and acculturative stress : associations with hazardous alcohol use among Asian American college students 显示文摘 | Zamboanga BL Broun CM Jiang Y | 2012 | Alcoh Clin Exp Res2012，36，： | 1 |
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