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| 1 | Recognition of high-specificity hERG K+ channel inhibitor-induced arrhythmia in cardiomyocytes by automated template matching显示文摘Cardiovascular disease(CVD)is the number one cause of death in humans.Arrhythmia induced by gene mutations,heart disease,or hERG K+channel inhibitors is a serious CVD that can lead to sudden death or heart failure.Conventional cardiomyocyte-based biosensors can record extracellular potentials and mechanical beating signals.However,parameter extraction and examination by the naked eye are the traditional methods for analyzing arrhythmic beats,and it is difficult to achieve automated and efficient arrhythmic recognition with these methods.In this work,we developed a unique automated template matching(ATM)cardiomyocyte beating model to achieve arrhythmic recognition at the single beat level with an interdigitated electrode impedance detection system.The ATM model was established based on a rhythmic template with a data length that was dynamically adjusted to match the data length of the target beat by spline interpolation.The performance of the ATM model under long-term astemizole,droperidol,and sertindole treatment at different doses was determined.The results indicated that the ATM model based on a random rhythmic template of a signal segment obtained after astemizole treatment presented a higher recognition accuracy(100%for astemizole treatment and 99.14%for droperidol and sertindole treatment)than the ATM model based on arrhythmic multitemplates.We believe this highly specific ATM method based on a cardiomyocyte beating model has the potential to be used for arrhythmia screening in the fields of cardiology and pharmacology. | Hao Wang Hongbo Li Xinwei Wei Tao Zhang Yuting Xiang Jiaru Fang Peiran Wu Xi Xie Ping Wang Ning Hu | 2021 | Microsystems & Nanoengineering2021，7，2： | 2 |
| 2 | Semi-Implantable Bioelectronics显示文摘Developing techniques to effectively and real-time monitor and regulate the interior environment of biological objects is significantly important for many biomedical engineering and scientific applications, including drug delivery, electrophysiological recording and regulation of intracellular activities. Semi-implantable bioelectronics is currently a hot spot in biomedical engineering research area, because it not only meets the increasing technical demands for precise detection or regulation of biological activities, but also provides a desirable platform for externally incorporating complex functionalities and electronic integration. Although there is less definition and summary to distinguish it from the well-reviewed non-invasive bioelectronics and fully implantable bioelectronics, semi-implantable bioelectronics have emerged as highly unique technology to boost the development of biochips and smart wearable device. Here, we reviewed the recent progress in this field and raised the concept of “Semi-implantable bioelectronics”, summarizing the principle and strategies of semi-implantable device for cell applications and in vivo applications, discussing the typical methodologies to access to intracellular environment or in vivo environment, biosafety aspects and typical applications. This review is meaningful for understanding in-depth the design principles, materials fabrication techniques, device integration processes, cell/tissue penetration methodologies, biosafety aspects, and applications strategies that are essential to the development of future minimally invasive bioelectronics. | Jiaru Fang Shuang Huang Fanmao Liu Gen He Xiangling Li Xinshuo Huang Hui-jiuan Chen Xi Xie | 2022 | Nano-Micro Letters2022，14，7： | 1 |
| 3 | Cardiomyocyte electrical-mechanical synchronized model for high-content, dose-quantitative and time-dependent drug assessment显示文摘Cardiovascular diseases have emerged as a significant threat to human health.However,drug development is a time-consuming and costly process,and few drugs pass the preclinical assessment of safety and efficacy.The existing patch-clamp,Ca2+imaging,and microelectrode array technologies in cardiomyocyte models for drug preclinical screening have suffered from issues of low throughput,limited long-term assessment,or inability to synchronously and correlatively analyze electrical and mechanical signals.Here,we develop a high-content,dose-quantitative and time-dependent drug assessment platform based on an electrical-mechanical synchronized(EMS)biosensing system.This microfabricated EMS can record both firing potential(FP)and mechanical beating(MB)signals from cardiomyocytes and extract a variety of characteristic parameters from these two signals(FP–MB)for further analysis.This system was applied to test typical ion channel drugs(lidocaine and isradipine),and the dynamic responses of cardiomyocytes to the tested drugs were recorded and analyzed.The high-throughput characteristics of the system can facilitate simultaneous experiments on a large number of samples.Furthermore,a database of various cardiac drugs can be established by heat map analysis for rapid and effective screening of drugs.The EMS biosensing system is highly promising as a powerful tool for the preclinical development of new medicines. | Jiaru Fang Xinwei Wei Hongbo Li Ning Hu Xingxing Liu Dongxin Xu Tao Zhang Hao Wan Ping Wang Xi Xie | 2021 | Microsystems & Nanoengineering2021，7，2： | 1 |
| 4 | Tumor-on-a-chip:from bioinspired design to biomedical application显示文摘Cancer is one of the leading causes of human death,despite enormous efforts to explore cancer biology and develop anticancer therapies.The main challenges in cancer research are establishing an efficient tumor microenvironment in vitro and exploring efficient means for screening anticancer drugs to reveal the nature of cancer and develop treatments.The tumor microenvironment possesses human-specific biophysical and biochemical factors that are difficult to recapitulate in conventional in vitro planar cell models and in vivo animal models.Therefore,model limitations have hindered the translation of basic research findings to clinical applications.In this review,we introduce the recent progress in tumor-on-a-chip devices for cancer biology research,medicine assessment,and biomedical applications in detail.The emerging tumor-on-a-chip platforms integrating 3D cell culture,microfluidic tech no logy,and tissue engineeri ng have successfully mimicked the pivotal structural and functional characteristics of the in vivo tumor microenvironment.The recent advances in tumor-on-a-chip platforms for cancer biology studies and biomedical applications are detailed and an a lyzed in this review.This review should be valuable for further understanding the mechanisms of the tumor evolution process,screening anticancer drugs,and developing cancer therapies,and it addresses the challenges and potential opportunities in predicting drug screening and cancer treatment. | Xingxing Liu Jiaru Fang Shuang Huang Xiaoxue Wu Xi Xie Ji Wang Fanmao Liu Meng Zhang Zhenwei Peng Ning Hu | 2021 | Microsystems & Nanoengineering2021，7，3： | 0 |
| 5 | Integrated Au-Nanoroded Biosensing and Regulating Platform for Photothermal Therapy of Bradyarrhythmia显示文摘Bradyarrhythmia is a kind of cardiovascular disease caused by dysregulation of cardiomyocytes,which seriously threatens human life.Currently,treatment strategies of bradyarrhythmia mainly include drug therapy,surgery,or implantable cardioverter defibrillators,but these strategies are limited by drug side effect,surgical trauma,and instability of implanted devices.Here,we developed an integrated Au-nanoroded biosensing and regulating platform to investigate the photothermal therapy of cardiac bradyarrhythmia in vitro.Au-nanoroded electrode array can simultaneously accumulate energy from the photothermal regulation and monitor the electrophsiological state to restore normal rhythm of cardiomyocytes in real time.To treat the cardiomyocytes cultured on Au-nanoroded device by near-infrared(NIR)laser irradiation,cardiomyocytes return to normal for long term after irradiation of suitable NIR energy and maintenance.Compared with the conventional strategies,the photothermal strategy is more effective and convenient to regulate the cardiomyocytes.Furthermore,mRNA sequencing shows that the differential expression genes in cardiomyocytes are significantly increased after photothermal strategy,which are involved in the regulation of the heart rate,cardiac conduction,and ion transport.This work establishes a promising integrated biosensing and regulating platform for photothermal therapy of bradyarrhythmia in vitro and provides reliable evidence of photothermal regulation on cardiomyocytes for cardiological clinical studies. | Jiaru Fang Dong Liu Dongxin Xu Qianni Wu Hongbo Li Ying Li Ning Hu | 2022 | Research2022，，3： | 0 |
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