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| 1 | Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products显示文摘The methylotrophic yeast Pichia pastoris(a.k.a.Komagataella phaffii)is one of the most commonly used hosts for industrial production of recombinant proteins.As a non-conventional yeast,P.pastoris has unique biological characteristics and its expression system has been well developed.With the advances in synthetic biology,more efforts have been devoted to developing P.pastoris into a chassis for the production of various high-value compounds,such as natural products.This review begins with the introduction of synthetic biology tools for the engineering of P.pastoris,including vectors,promoters,and terminators for heterologous gene expression as well as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated System(CRISPR/Cas)for genome editing.This review is then followed by examples of the production of value-added natural products in metabolically engineered P.pastoris strains.Finally,challenges and outlooks in developing P.pastoris as a synthetic biology chassis are prospected. | Jucan Gao Lihong Jiang Jiazhang Lian | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 9 |
| 2 | Coating thick MgB_(2) layer on stainless steel substrate显示文摘 | CHENChinping FENGQingrong GANZizhao XlONGGuangcheng XUJun LIUYufei KONGLingwen LILin JIAZhang GUOJingpu ZHUANGChenggang DINGLili CHENLiping LIFen ZHANGKaicheng | 2005 | Chinese Science Bulletin2005，50，7： | 5 |
| 3 | A reappraisal of the relationship between metatarsus adductus and hallux valgus显示文摘 | Chen Li Wang Chen Wang Xu Huang Jiazhang Zhang Chao Zhang Yijun Ma Xin | 2014 | Chinese Medical Journal2014，，11： | 4 |
| 4 | Identification of Peptides Inhibiting Adhesion of Monocytes to the Injured Vascular Endothelial Cells through Phage-displaying Screening显示文摘Using oxidized low-density lipoprotein (LDL)-injured vascular endothelial cells (ECs) as target cells, peptides specifically binding to the injured ECs were screened from a phage-displaying peptide library by using the whole-cell screening technique after three cycles of the ''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''adsorption-elution-amplification'''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''' procedure. Positive phage clones were identified by ELISA, and the inserted amino acid sequences in the displaying peptides were deduced from confirmation with DNA sequencing. The adhesion rate of ECs to monocytes was evaluated by cell counting. The activity of endothelial nitric oxide synthase (eNOS), and the expression levels of caveolin-1 and intercellular adhesion molecule-1 (ICAM-1) were determined by Western blotting. Six positive clones specifically binding to injured ECV304 endothelial cells were selected from fourteen clones. Interestingly, four phages had peptides with tandem leucine, and two of these even shared an identical sequence. Functional analysis demonstrated that the YCPRYVRRKLENELLVL peptide shared by two clones inhibited the expression of ICAM-1, increased nitric oxide concentration in the culture media, and upregulated the expression of caveolin- 1 and eNOS. As a result, the adhesion rate of monocytes to ECV304 cells was significantly reduced by 12.1%. These data suggest that the anti-adhesion effect of these novel peptides is related to the regulation of the caveolin- 1/nitric oxide signal transduction pathway, and could be of use in potential therapeutic agents against certain cardiovascular diseases initiated by vascular endothelial cell damage. | YuGUO JiaZHANG Ji-ChengWANG Feng-XiangYAN Bing-YangZHU Hong-LinHUANG Duan-FangLIAO | 2005 | Acta Biochimica et Biophysica Sinica2005，37，4： | 3 |
| 5 | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology显示文摘Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade.However,due to complexity of cellular metabolism,the optimization of metabolic pathways for maximal production represents a grand challenge and an unavoidable barrier for metabolic engineering.Recently,cell-free protein synthesis system(CFPS)has been emerging as an enabling alternative to address challenges in biomanufacturing.This review summarizes the recent progresses of CFPS in rapid prototyping of biosynthetic pathways and genetic circuits(biosensors)to speed up design-build-test(DBT)cycles of metabolic engineering and synthetic biology. | Lihong Jiang Jiarun Zhao Jiazhang Lian Zhinan Xu | 2018 | Synthetic and Systems Biotechnology2018，3，2： | 3 |
| 6 | Exo^+ proofreading polymerases mediate genetic analysis and its application in biomedical studies显示文摘Polymerases with a proofreading function in their internal 3' to 5' exonuclease possess high fidelity for DNA replication both in vivo and in vitro. The obstacle facing Exo^+ polymerases for single nucleotide polymorphism (SNP) detection could be bypassed by using primer-3'-termini modification. This hypothesis has been well tested using three types of modified allele specific primers with: 3' labeling, 3'to 5'exonuclease resistance, and 3'dehydroxylation. Accordingly, three new SNP assaying methods have been developed to carry out genome-wide genotyping, taking advantage of the enzymatic properties of Exo^+ polymerases. These new mutation detection assays are widely adaptable to a variety of platforms, including multi-well plate and microarray technologies. Application of Exo^+ polymerases to genetic analysis, including genotyping that is mostly relevant to pharmacogenetics, high-fidelity gene expression profiling, rare mutation detection and mutation load assay, will help to accelerate the pace of personalized medicine. In this review paper, we will first introduce three new assays that we have recently developed, and then describe a number of their applications in pharmacogenetics and in other biomedical studies. | Duan-fangLIAO Lin-lingCHEN Cui-yingPENG JiaZHANG KaiLI | 2005 | Acta Pharmacologica Sinica2005，26，3： | 2 |
| 7 | A unique deubiquitinase that deconjugates phosphoribosyl-linked protein ubiquitination显示文摘Ubiquitination 调整主人免疫的许多方面并且因此是为传染代理人的一个普通目标。最近的研究表明了细菌的病原体 Legionella pneumophila 的方面受动器家庭的成员攻击不要求主人 ubiquitination 机械的 E1 和 E2 酶的新奇 ubiquitination 机制与 endoplasmic 蜂窝胃联系的几小 GTPases。在这种情况中, ubiquitin 被一项 mono-ADP-ribosyltransferase 活动被 ADP-ribosylation 首先在 Arg 42 激活;中介然后被也住在 SdeA 以内的一项 phosphodiesterase 活动劈开,有到经由一个 phosphoribosyl 连接器的底层蛋白质的丝氨酸残余的 ubiquitin 的附件的伴随物。这里,我们证明方面的效果被 SidJ 反对,一个受动器由位于为方面家庭(SdeC, SdeB 和 SdeA ) 的三个成员编码的地点的基因编码了。SidJ 由劈开连接 phosphoribosylated ubiquitin 到蛋白质底层的 phosphodiester 契约颠倒修改方面的底层的 ubiquitination。SidJ 也显示古典 deubiquitinase 活动但是不要求催化半胱氨酸残余。进一步, SidJ 的这些 deubiquitinase 活动为它在 L 的角色是必要的。pneumophila 感染。最后, SidJ 的活动为高效地在细菌的举起以后在一些小时以内在感染的房间减少许多 ubiquitinated Rab33b 被要求。我们的结果作为工作在方面受动器的活动强加时间的规定的 ubiquitin-deconjugating 酶建立 SidJ。SidJ 可能在表明串联的未来研究是重要的由这唯一的 ubiquitination 调停了,潜在地也在真核细胞的房间调整细胞的过程的。 | Jiazhang Qiu Kaiwen Yu Xiaowen Fei Yao Liu Emesto S Nakayasu Paul D Piehowski Jared B Shaw Kedar Puva Chittaranjan Das Xiaoyun Liu Zhao-Qing Luo | 2017 | Cell Research2017，27，7： | 2 |
| 8 | Research Progress of Rice-Fish Integrated Farming显示文摘Rice-fish integrated farming( RFIF) can change the agricultural production pattern from mono-farming model to the co-farming model,and the stereoscopic and integrated farming system established by RFIF can change the flat production to the stereoscopic production. Therefore,RFIF is the important development direction for agriculture to change pattern and adjust structure. In order to further promote development of the research and application of RFIF,the general development situation of RFIF in China was reviewed; the effects of RFIF on increasing farmer' income and enhancing agricultural efficiency were clarified. The technologies matched with RFIF,such as rice paddy engineering technology,safe plant protection technology,security fertilization technology,fish disease prevention and control technology and water quality management technology,were summarized,and the supporting role of RFIF for agriculture was pointed out. In addition,the development prospect of RFIF was proposed,as well as the key research direction of RFIF. | Shunlong MENG Gengdong HU DANDan LI Liping QIU Chao SONG Limin FAN Yao ZHENG Wei WU Jiazhang CHEN Xuwen BING | 2018 | Agricultural Biotechnology2018，7，4： | 2 |
| 9 | Environmental lead pollution threatens the children living in the Pearl River Delta region, China显示文摘 | Jianmin Chen Yongpeng Tong Jiazhang Xu Xiaoli Liu Yulan Li Mingguang Tan Yan Li | 2012 | Environmental Science and Pollution Research2012，，8： | 1 |
| 10 | Environmental lead pollution threatens the children living in the pearl fiver delta region 显示文摘 | JIANMIN C YONGPENG T JIAZHANG X | 2012 | China Environment Science and Pollution Research2012，19，8： | 1 |
| 11 | Eugenol Reduces the Expression of Virulence-Related Exopro- teins in Staphylococcus aureus 显示文摘 | Jiazhang Qiu Haihua Feng Jing Lu | 2010 | Applied And En- vironmental Microbiology2010，，9： | 1 |
| 12 | Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae显示文摘 | Jiazhang Lian Yanglin Li Mohammad HamediRad Huimin Zhao | 2014 | Biotechnol. Bioeng2014，，8： | 1 |
| 13 | Superconducting Magnetic Flux Transformer at 77K显示文摘Superconducting Quantum InterferenceDevice(SQUID)magnetometer is a kind ofmagnetic flux sensor with high sensitivity,andtherefore can be used in geological prospectand biomagnetic field etc..But the SQUIDmagnetometer used before has to be used atthe temperature of 4.2K provided by liquidhelium and thus its application is limited. | Ren, Hongtao Xiao, Ling He, Qing Li, Jiazhang | 1990 | Rare Metals1990，10，1： | 0 |
| 14 | Biomanufacturing ofγ-linolenic acid-enriched galactosyldiacylglycerols:Challenges in microalgae and potential in oleaginous yeasts显示文摘γ-Linolenic acid-enriched galactosyldiacylglycerols(GDGs-GLA),as the natural form ofγ-linolenic acid in microalgae,have a range of functional activities,including anti-inflammatory,antioxidant,and anti-allergic properties.The low abundance of microalgae and the structural stereoselectivity complexity impede microalgae extraction or chemical synthesis,resulting in a lack of supply of GDGs-GLA with a growing demand.At present,there is a growing interest in engineering oleaginous yeasts for mass production of GDGs-GLA based on their ability to utilize a variety of hydrophobic substrates and a high metabolic flux toward fatty acid and lipid(triacylglycerol,TAG)production.Here,we first introduce the GDGs-GLA biosynthetic pathway in microalgae and challenges in the engineering of the native host.Subsequently,we describe in detail the applications of oleaginous yeasts with Yarrowia lipolytica as the representative for GDGs-GLA biosynthesis,including the development of synthetic biology parts,gene editing tools,and metabolic engineering of lipid biosynthesis.Finally,we discuss the development trend of GDGs-GLA biosynthesis in Y.lipolytica. | Xiaosong Gu Lei Huang Jiazhang Lian | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
| 15 | A New Chemical Trick to Prevent House from Collapsing by Bacterial Pathogens显示文摘Metazoans often induce cell death as an ultimate strategy to prevent disastrous damage to the body under circumstances when insults,such as infection are impossible to clear.Inflammatory cell death or pyroptosis not only eliminates the replicative niche for the pathogen but also stimulates effective immune protection.A recent study by Shao,Liu and colleagues found that the Shigella flexneri effector OspC3 inactivates an inflammasome by a novel posttranslational modification termed ADP-riboxanation.This finding has greatly expanded the chemical tool box of pathogens in their manipulation of host function,which may be used to probe unrecognized signaling mechanisms. | QIU Jiazhang LUO Zhao-Qing | 2022 | Chemical Research in Chinese Universities2022，38，2： | 0 |
| 16 | Review on Pond Aquaculture Tail Water Treatment Technologies显示文摘Carrying out the treatment of aquaculture tail water and realizing the discharge and recycling of the aquaculture tail water are the inevitable trend in the development of the fishery industry at present and in the future.For this reason,this paper reviewed the characteristics of pond aquaculture tail water,the sources of pollutants in intensive aquaculture tail water,the important parameters in the water body that affect the growth of cultured organisms,and the water treatment methods in the process of aquaculture,and prospected from the technical level and industrial policy level,hoping to accumulate data for promoting the green development of aquaculture and cleaner production. | Shunlong MENG Lihua REN Xi CHEN Liping QIU Chao SONG Limin FAN Dandan LI Jiazhang CHEN Pao XU | 2022 | Agricultural Biotechnology2022，11，2： | 0 |
| 17 | Formation of Omega-like Nanocrystalline in the Melt-Spun Nd_(85)Al_(15) Alloy by Phase Transformation显示文摘Microstructure and subsequent phase transformations on heating of the melt-spun Nd85Al15 alloy have been studied by X-ray diffraction,transmission electron microscopy and differential scanning calorimetry.The melt-spun Nd85Al15 alloy shows two-stage transformation processes as follows:amorphous+72 nm supersaturated bcc-Nd(Al) solid solution→7 nm omega-like phase→AlNd3+hexagonal Nd.The activation energies for the first and second transformation were found to be 100kJ/mol and 188kJ/mol,respectively.The formation mechanism of nanoscale omega-like phase is discussed. | JiaZHANG WenshengSUN MingxiuQUAN ZhuangqiHU | 2002 | Journal of Materials Science & Technology2002，18，5： | 0 |
| 18 | Metabolic engineering:Tools and applications显示文摘Metabolic engineering plays a pivotal role in the development of mi-crobial cell factories for efficient production of biofuels,chemicals,and natural products,which facilitate the transition from fossil-resource de-pendent processes to green and sustainable bio-based processes.By do-ing so,we could address global challenges such as sustainability,human health,and climate change. | Yun Chen Jiazhang Lian Jin Hou | 2023 | Engineering Microbiology2023，3，4： | 0 |
| 19 | Testis of Male Tilapia Under Methomyl Stress: Transcriptome Changes and Signal Pathway Analysis显示文摘This study was conducted to find the differentially expressed genes and understand the transcriptome change characteristics in the testis of tilapia(Oreochromis niloticus) stressed by methomyl.The tilapia were exposed to 200 μg/L methomyl for 30 d,and then the testis samples were collected and used for the highthroughput sequencing.The results showed that 369 significant differentially expressed genes were obtained,including 160 significantly down-regulated genes,and209 significantly up-regulated genes.The enrichment of the differentially expressed genes in ECM-receptor interaction signaling pathway and MAPK signaling pathway was obvious.The adhesion of testis tissue cells was influenced by methomyl stress,which induced the expression of related gene in ECM-receptor interaction up-regulating,such as laminins,integrin α6 and integrin β1,in order to stabilize testis tissue structure.JNK,MAP3 K and MKK4 in the JNK pathway were significantly up-regulated,which enhanced transcription factor AP-1 to promote the expression of P53,Bax and other pro-apoptotic proteins.The expression of growth-related factors in the ERK pathway of MAPK signal pathway was influenced by methomyl stress.The apoptosis-related genes and pregnancy-related genes have significant differential expression under methomyl stress,and thereby,the proliferation of reproduction cells in the testis tissue may be affected. | Shunlong MENG Tao LIU Chao SONG Cong ZHANG Jiazhang CHEN | 2020 | Agricultural Biotechnology2020，9，2： | 0 |
| 20 | The Effects of Environmental Factors on the Growth and Competition of Algae显示文摘In order to study the effect of environmental factors on the algae growth and competition,the author summarized overseas and domestic related researches in recent years.Most of the researches are about the influence of single factor on growth of algae.However,there is insufficient investment on the interaction of different factors and the competition between algae growth.This paper briefly introduced the classification of algae and the role they played in ecological system and focused on the influence which included temperature,illumination,nitrogen,phosphorus and pH on the growth and competition of algal.In the end,the author proposed key questions which were still needed to be studied in order to know more about the relationship between environment effects and growth and competition of algae.Therefore,people could better improve the community structure of algae and water ecological environment,and improve water primary productivity. | Jing WANG Jiazhang CHEN Shunlong MENG | 2013 | Asian Agricultural Research2013，5，12： | 0 |
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