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| 1 | Development of an oral DNA vaccine against MG7-Ag of gastric cancer using attenuated salmonella typhimurium as carrier显示文摘AIM: To develop an oral DNA vaccine against gastric cancer and evaluate its efficacy in mice.METHODS: The genes of the MG7-Ag mimotope and a universal Th epitope (Pan-DR epitope, PADRE) were included in the PCR primers. By PCR, the fusion gene of the two epitopes was amplified. The fusion gene was confirmed by sequencing and was then cloned into pcDNA3.1(+) plasmid. The pcDNA3.1 (+)-MG7/PADRE was used to transfect an attenuated Salrmonella typhimuriurm.C57BL/6 mice were orally immunized with 1x108 cfu Salrmonella transfectants. Salmonella harboring the empty pcDNA3.1(+) plasmid and phosphate buffer saline (PBS)were used as negative controls. At the 6th week, serum titer of MG7-Ag specific antibody was detected by ELtSA.At the 8th week cellular immunity was detected by an unprimed proliferation test of the spleenocytes by using a [3H]-thymidine incorporation assay. Ehrlich ascites carcinoma cells expressing MG7-Ag were used as a model in tumor challenge assay to evaluate the protective effect of the vaccine.RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than that in control groups (0.841 vs 0.347, P<0.01; 0.841 vs 0.298,P<0.01), while in vitro unprimed proliferation assay of the spleenocytes showed no statistical difference between those three groups. Two weeks after tumor challenge, 2 in 7 immunized mice were tumor free, while all the mice in the control groups showed tumor formation.
CONCLUSION: Oral DNA vaccine against the MG7-Ag momitope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumor in mice, and the vaccine has partially protective effects. | Quan-LiHan Fan-PingMeng NaLiu Dai-MingFan Chang-CurGuo JieDing Bo-RongPan Zhao-CaiYu | 2003 | World Journal of Gastroenterology2003，9，6： | 12 |
| 2 | Oral attenuated Salmonella typhimurium vaccine against MG7-Ag mimotope of gastric cancer显示文摘AIM: To develop an oral attenuated Salmonella typhimurium vaccine against gastric cancer and to evaluate its efficacy in mice.METHODS: A complementary sequence of Nco I site and a sequence coding for MG7-Ag mimotope were designed at the 5' terminus of forward primer. Using p1.2 Ⅱ-HBCAg plasmid as template, PCR was performed to get a fusion gene of the mimotope and a HBcAg gene. The fusion gene was then subcloned into the plasmid pYA3341complementary to Salmonella typhimurium X4550, and the recombinant plasmid was then transformed into attenuated Salmonella typhimurium X4550. Balb/c mice were orally immunized with the recombinant Salmonella typhimurium X4550. The mice were immunized every 2 wk to reinforce the immunity. At the 6th wk, serum titer of antibody was detected by ELISA, and at the 8th wk,cellular immunity was detected by 51Cr release test. Ehrlich ascites carcinoma cells expressing MG7-Ag were used in tumor challenge assay as a model to evaluate the protective effect of the vaccine.RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than in control groups (0.9538±0.043 vs0.6531±0.018,P<0.01; 0.9538±0.043 vs0.6915±0.012, P<0.01), while in vitro 51Cr release assay of the splenocytes showed no statistical difference in the three groups. Two weeks after tumor challenge, 1 in 5 immunized mice was tumor free, while all the mice in the control group presented tumor.CONCLUSION: Oral attenuated Salmonella typhimurium vaccine against the MG7-Ag mimotope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumors in mice, and has some protective effects. | Fan-PingMeng JieDing Zhao-CaiYu Quan-LiHan Chang-CunGuo NaLiu Dai-MingFan | 2005 | World Journal of Gastroenterology2005，11，12： | 6 |
| 3 | Postoperative endoscopic surveillance of human living-donor small bowel transplantations显示文摘AIM: To determine the significance of endoscopic surveillance in the diagnosis of acute rejection after human living-donor small bowel transplantations.METHODS: Endoscopic surveillance was performed through the ileostomy after human living-donor small bowel transplantations. The intestinal mucosa was observed and biopsies were performed for pathological observations.RESULTS: Acute rejection was diagnosed in time by endoscopic surveillance. The endoscopic and pathological manifestations of acute rejection were described. CONCLUSION: Endoscopic surveillance and biopsy are reliable methods to diagnose the acute rejection after human living-donor small bowel transplantations. | JieDing Chang-CunGuo Cai-NingLi An-HuaSun Xue-GangGuo Ji-YanMiao Bo-RongPan | 2003 | World Journal of Gastroenterology2003，9，3： | 2 |
| 4 | Meta‐analysis of laparoscopy‐assisted distal gastrectomy with D2 lymph node dissection for gastric cancer显示文摘 | JieDing Guo‐QingLiao He‐LiLiu ShengLiu JingTang | 2012 | J. Surg. Oncol2012，，3： | 2 |
| 5 | Meta‐analysis of laparoscopy‐assisted distal gastrectomy with D2 lymph node dissection for gastric cancer显示文摘 | JieDing Guo‐QingLiao He‐LiLiu ShengLiu JingTang | 2012 | J Surg Oncol2012，，3： | 1 |
| 6 | Helicobacter pylori specific immune response induced by conservative flagellin linear B-cell epitope显示文摘AIM:To testify the immunogenicity of a conservative B-cell linear epitope of Helicobacter pylori ( H pylori) flagellin A. METHODS: Different programs were used to analyze the secondary structure, molecular hydropathy, and surface accessibility of Hpyloriflagellin A. Linear B-cell epitopes were estimated based on the structural and physiochemical information. Analysis of residue divergence was proposed to screen a conservative linear epitope. The 29-peptide (Pep29mer) synthesized by chemical method, including the predicted conservative B-cell epitope and a known K^2d compatible T-cell epitope, was used to immunize mice, and then H pylori-specific antibodies were detected by ELISA. RESULTS: Based on the analyses of divergent amino acid residues, structural and physiochemical characteristics, it was strongly suggested that the short fragment NDSDGR was the core of a conservative linear epitope in flagellin A. Animals immunized by Pep29mer acquired efficient immune response. In detail, serum Hpylori-specific IgA and IgGl increased significantly in immunized group, while IgG2a only had an insignificant change. Hpylori-specific IgA in gastrointestinal flushing fluid also increased significantly. CONCLUSION: The conservative short fragment NDSDGR is the core of a linear B-cell epitope of flagellin A. | Wan-ShengJi Jia-LuHu Kai-ChunWu Jun-WenQiu Zhe-YiHan JieDing Dai-MingFan | 2005 | World Journal of Gastroenterology2005，11，23： | 1 |
| 7 | An experimental study on downward slug flow in inclined pipes显示文摘 | JIEDE Yang CHEN X T BRILL J P | 1996 | SPE J1996，，8： | 1 |
| 8 | Black to Green显示文摘Devastated by years of unchecked llegal mining,western China's Muli coalfeld faces funding shortages and a slew of other challenges in restoring the local environment Wang Qiang watched as the convoy of coal mining trucks crisscrossed the pastures.They were his trucks,but instead of fossil fuel,they hauled loads of sheep manure. | Su Jiede | 2021 | China Weekly2021，，5： | 0 |
| 9 | An oral DNA vaccine against MG7—Ag of gastric cancer using attenuated Salmonella typhimurium as carrier显示文摘Aims:To develop an oral DNA vaccine against gastric cancer and evaluate its efficacy in mice.Methods:The gene of the MG7-Ag mimotope and a universal Th epitop (Pan-DR epitope,PADRE) were included in the PCR primers.By PCR,the fusion gene of the two epitopes was amplified.The fusion gene was confirmed by sequencing and then cloned into pcDNA3.1(+) plasmid.The pcDNA3.1(+)-MG7/PADRE was used to transfect an anttenuatedf Salmonella typhimurium.C57BL/6 mice were orally immunized uated Salmonella typhimurium.C57BL/6 micr were orally immunized with 1×10^8 cfu Salmonella transfectants.Salmonella harboring the empty pcDNA3.1(+) plasmid and phosphate buffer saline (PBS) were used as negative control.At the 6th week,serum titer of MG7-Ag specific antibody was detected by ELISA.at the 8th week cellular immunity was detected by an unprimed proliferation test of the spleenocytes by using a [^3H]-thymidine incorporation assay.Ehrlich ascites carcinoma cells expressing MG7-Ag were used as a model in tumor challenge assay to evaluate the protective of the vaccine.Results:Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than control groups (0.841 vs 0.374,P<0.01;0.841 vs 0.298,P<0.01),while exvivo unprimed proliferation assay of the spleenocytes showed no statistical difference between those three groups.Two weeks after tumor challenge,2 in 7 immunized mice were tumor free,while all the mice in the control groups showed tumor formation.Conclusions:Oral DNA vaccine against the MG7-Ag mimotope of gastric cancer is immunogenic.It can induce significant humoral immunity against tumor in mice,and the vaccine has partially protective effects. | ChangCunGuo JieDing 等 | 2002 | 胃肠病学2002，7，B11： | 0 |
| 10 | Small Good,Bingger Plans显示文摘Merchants in Yiwn,Zhejiang Province,a city known across the globe for its small commodity manufacturing and wholesales,seemed convinced that incumbent US President Donald Trump was going to win relection,judging by the way Trump merchandise like Aags,hats and T-shirts was flying off the shelves.According to media reports,orders for Trump branded merch outnumbered those for his opponent,former US vice president and now President elect Joe Biden,as much as five to one. | Su Jiede | 2021 | China Weekly2021，，1： | 0 |
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