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| 1 | TGF-β promotes invasion and metastasis of gastric cancer cells by increasing fascinl expression via ERK and JNK signal pathways显示文摘 | Hua Fu Zhongliang Hu Jifang Wen Kuansong Wang Ying Liu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 19 |
| 2 | The cloning of Dmc1 cDNAs and a comparative study of its expression in different ploidy cyprinid fishes显示文摘Dmc1 (disrupted meiotic cDNA) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. In this investigation, we cloned the partial cDNAs of Dmc1 of diploid red crucian carp, Japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the DMC1 protein in yeast, mouse and human. The full length cDNAs were then obtained by rapid amplification of cDNA ends (RACE). Our data showed that the full length cDNAs of Dmc1 in the three diploid fishes are all 1375 bp long, while it is 1383 bp long in triploids and 1379 bp long in allotetraploids. And despite of the variation in length, all the cDNAs encode a protein of 342 amino acids. A high homology of 97.3% of the DMC1 protein can be drawn by comparing the amino acid sequences in the three diploids, which is also of 86%, 86% and 95% similarity to human, mouse and zebrafish, respectively. A comparative study of the expression pattern of Dmc1 was carried out by RT-PCR using specific primers against the same se-quences of coding regions in different ploidy cyprinid fishes, from which it was showed that Dmc1 was expressed only in gonads of these five kinds of fishes. The expression pattern of Dmc1 in both ovaries and testes from different ploidy fishes within breeding season was also studied by Real-time PCR, and the results showed that the expression of this gene was greatly different among the three different ploidy fishes, which was the highest of triploid and lowest of allotetraploids. The histological sections data showed matured gonads of both diploid red crucian carp and allotetraploids in breeding season, although the latter demonstrated a higher maturation, and no gonadal maturation could be observed in triploids. In conclusion, we suggest that Dmc1 is specifically expressed in the period of meiosis in all the ploidy cyprinid fishes and directly related with the development of gonad in a manner of ploidy-independent way. And further, the high expression of Dmc1 in female triploids might be associ-ated with abnormal meiosis and sterility. | TAO Min, LIU ShaoJun, LONG Yu, ZENG Chen, LIU JiFang, LIU LiangGuo, ZHANG Chun, DUAN Wei & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，1： | 13 |
| 3 | Enhanced Invasive and Metastatic Potential Induced by Transforming Growth Factor-β1 Might be Correlated with Glutathione-S-transferase-π, Cofilin and Heat Shock Protein 27 in SGC-7901 Gastric Cancer Cells显示文摘在我们的以前的学习,我们发现转变生长因素(TGF )-beta1 提高了胃的癌症房间的变形、侵略的潜力。Proteomics 在这研究被采用进一步说明内在的分子的机制。Aftertwo 维的电气泳动,图象分析,点鉴定,蛋白质鉴定和数据库分析,三蛋白质也就是, glutathione-S-transferase-pi (GST-pi ) , cofilin 和热震惊蛋白质 27 (HSP27 ) ,被发现在 TGF-beta1 起来调整对待的 SGC-7901 房间。Thefindings 被蛋白质印迹分析进一步证实。这些结果建议 GST-pi, cofilin 和 HSP27 可能参予 TGF-beta1 导致的提高的侵略潜力。 | Kuansong WANG Jinghe LI Changli ZHEN Jianhua ZHOU Desheng XIAO Jie LIU Ying LIU Haiying JIANG Chen CHEN Jifang WEN | 2007 | Acta Biochimica et Biophysica Sinica2007，39，7： | 11 |
| 4 | RESEARCH ON ZERO CLEARANCE CYCLOID BALL TRANSMISSION显示文摘RESEARCHONZEROCLEARANCECYCLOIDBALLTRANSMISSIONQuJifangAnZijunYanshanUniversityAbstract:Thesteelballtransmissionofzerogearclea... | Qu Jifang An Zijun Yanshan University | 1994 | Chinese Journal of Mechanical Engineering1994，7，1： | 9 |
| 5 | Practices on rockburst prevention and control in headrace tunnels of Jinping II hydropower station显示文摘Rockburst problems induced by high in-situ stresses were prominent during construction of the headrace tunnels of Jinping II hydropower station. The rockbursts occurred in various forms, and it is necessary to take pertinent measures for integrated prevention and control of rockbursts. In view of the rockburst characteristics during tunnel construction of Jinping II hydropower station, the engineering geological conditions were presented, and the features, mechanisms and forms of rockbursts observed during construction were analyzed in detail. A large number of scientific researches, experiments and applications were conducted. Multiple measures were adopted to prevent and control rockbursts, including the prediction and early warning measures, stress relief by blasting in advance, optimized blasting design and optimized tunnel support in the tunnel sections prone to strong rockbursts. The effectiveness of these prevention and control measures was evaluated. Experiences have been accumulated through a great number of helpful explorations and practices for rockburst prevention and control. A comprehensive rockburst prevention and control system has been gradually established. | Jimin Wang Xionghui Zeng Jifang Zhou | 2012 | Journal of Rock Mechanics and Geotechnical Engineering2012，4，3： | 8 |
| 6 | Recent advances in designing conductive hydrogels for flexible electronics显示文摘Flexible electronics have emerged as an exciting research area in recent years,serving as ideal interfaces bridging biological systems and conventional electronic devices.Flexible electronics can not only collect physiological signals for human health monitoring but also enrich our daily life with multifunctional smart materials and devices.Conductive hydrogels(CHs)have become promising candidates for the fabrication of flexible electronics owing to their biocompatibility,adjustable mechanical flexibility,good conductivity,and multiple stimuli-responsive properties.To achieve on-demand mechanical properties such as stretchability,compressibility,and elasticity,the rational design of polymer networks via modulating chemical and physical intermolecular interactions is required.Moreover,the type of conductive components(eg,electron-conductive materials,ions)and the incorporation method also play an important role in the conductivity of CHs.Electron-CHs usually possess excellent conductivity,while ion-CHs are generally transparent and can generate ion gradients within the hydrogel matrices.This mini review focuses on the recent advances in the design of CHs,introducing various design strategies for electron-CHs and ion-CHs employed in flexible electronics and highlighting their versatile applications such as biosensors,batteries,supercapacitors,nanogenerators,actuators,touch panels,and displays. | Qiongyao Peng Jingsi Chen Tao Wang Xuwen Peng Jifang Liu Xiaogang Wang Jianmei Wang Hongbo Zeng | 2020 | InfoMat2020，2，5： | 8 |
| 7 | Clinical study of ultrasound and microbubbles for enhancing chemotherapeutic sensitivity of malignant tumors in digestive system显示文摘Objective: To explore the safety of ultrasound and microbubbles for enhancing the chemotherapeutic sensitivity of malignant tumors in the digestive system in a clinical trial, as well as its efficacy.Methods: From October 2014 to June 2016, twelve patients volunteered to participate in this study. Eleven patients had hepatic metastases from tumors of the digestive system, and one patient had pancreatic carcinoma.According to the mechanical index(MI) in the ultrasound field, patients were classified into four groups with MIs of 0.4, 0.6, 0.8 and 1.0. Within half an hour after chemotherapy, patients underwent ultrasound scanning with ultrasound microbubbles(SonoVue) to enhance the efficacy of chemotherapy. All adverse reactions were recorded and were classified in 4 grades according to the Common Terminology Criteria for Adverse Events version 4.03(CTCAE V4.03). Tumor responses were evaluated by the Response Evaluation Criteria in Solid Tumors version1.1 criteria. All the patients were followed up until progression.Results: All the adverse reactions recorded were level 1 or level 2. No local pain occurred in any of the patients.Among all the adverse reactions, fever might be related to the treatment with ultrasound combined with microbubbles. Six patients had stable disease(SD), and one patient had a partial response(PR) after the first cycle of treatment. At the end of follow-up, tumor progression was restricted to the original sites, and no new lesions had appeared.Conclusions: Our preliminary data showed the potential role of a combined treatment with ultrasound and microbubbles in enhancing the chemotherapeutic sensitivity of malignant tumors of the digestive system. This technique is safe when the MI is no greater than 1.0. | Yanjie Wang Yan Li Kun Yan Lin Shen Wei Yang Jifang Gong Ke Ding | 2018 | Chinese Journal of Cancer Research2018，30，5： | 8 |
| 8 | Some Investigations on Early Organization of Karst System显示文摘Some investigations on the early organization of karst system are carried out through modeling the dissolution evolution processes of stochastic fracture networks in carbonate rock. It is assumed that water flow in fracture network is laminar, and the dissolution rate process can be described with an empirical equation, The results suggest that a karst system is a self-organization system. It can spontaneously create higher hierarchical level out of a relative homogeneous structure through amplifying the microscopic heterogeneity of initial flow field. Under given boundary conditions, a karst system with initial fracture network of varied hierarchical levels is likely to have stronger tendency to lead to a typical karst system featuring conduit passages than a system with initial network of unique hierarchical level. Through merging local flow systems, a karst system concentrates the limited flow into a few pathways to promote the formation of higher hierarchical level. | Yu Qingchun(Geofront Laboratory, Department of Civil Engineering, Kyoto University, Kyoto 606-8501, Japan)Shen Jifang Wan Junwei(Department of Hydrogeology and Environmental Engineering, China University of Geosciences,Wuhan 430074, China)Ohnishi Yuzo(Geo | 1999 | Journal of Earth Science1999，18，4： | 8 |
| 9 | Inhibition of fibroblast growth factor 2-induced apoptosis involves survivin expression, protein kinase Cα activation and subcellular translocation of Smac in human small cell lung cancer cells显示文摘为了调查(FGF-2 ) 成纤维细胞生长因素 2 在人的小房间肺癌症房间线 H446 由禁止 apoptosis 的机制,使遭到了到浆液饥饿, apoptosis 被流动 cytometry,染色的 Hoechst 33258, caspase-3 活动,和 DNA 破碎评估。FGF-2 和蛋白质 kinase C (PKC ) translocation 导致的 Survivin 表示被 subcellular 分别和西方的污点分析检测。另外,到细胞质的从线粒体的 Smac 的 FGF-2-in-duced 版本被西方的弄污和 immunofluorescence 分析。FGF-2 减少了浆液饥饿和起来调整的 survivin 表示在剂量依赖者 andtime-dependentmanner 在 H446 房间导致的 apoptosis, andinhibitedcaspase-3 活动。FGF-2 也从线粒体禁止了 Smac 的版本到浆液饥饿导致并且从细胞质增加了 PKC translocation 到房间膜的细胞质。另外, PKC 禁止者禁止了 survivin 的表示。FGF-2 起来调整在 H446 房间和块的 survivin 蛋白质的表示到细胞质的从线粒体的 Smac 的版本。PKC 调整了 FGF-2-induced survivin 表示。因此, survivin, Smac,和 PKC 可能在人的小房间肺癌症房间由 FGF-2 在 apoptosis 的抑制起重要作用。 | Desheng Xiao Kuansong Wang Jianhua Zhou Huiqiu Cao Zhenghao Deng Yongbin Hu Xiahui Qu Jifang Wen | 2008 | Acta Biochimica et Biophysica Sinica2008，40，4： | 7 |
| 10 | Aneuploidy of chromosome 8 in circulating tumor cells correlates with prognosis in patients with advanced gastric cancer显示文摘Objective: Previous work indicated that aneuploidy of chromosome 8 in circulating tumor cells(CTCs)correlated with therapeutic efficacy for advanced gastric cancer(AGC) patients. In this follow-up study performed on the same population of AGC patients, we investigated whether and how aneuploidy of chromosome 8 in CTCs correlates with patients' clinical prognosis.Methods: The prospective study was performed on 31 patients with newly diagnosed AGC. Previously established integrated subtraction enrichment(SE) and immunostaining-fluorescence in situ hybridization(i FISH)platform was applied to identify, enumerate and characterize CTCs. Quantification of CTCs and analysis of their aneuploidy of chromosome 8 were performed on patients before and after therapy.Results: CTCs were measured in 93.5% of AGC patients, and two CTC subtypes with diverse threshold values were identified, multiploid CTCs with the threshold of ≥2 per 7.5 m L and multiploid plus triploid CTCs with the threshold of ≥4, which were found to significantly correlate with poor progression-free survival(PFS) and overall survival(OS). In particular, patients with ≥10% increased multiploid CTCs after an initial 6 weeks of therapy had poor PFS and OS, whereas improved PFS and OS were observed on those who had ≥10% decreased multiploid CTCs. After adjusting for clinically significant factors, ≥10% increased post-therapy multiploid CTCs was the only independent predictor of PFS and OS.Conclusions: Aneuploidy of CTCs correlates with prognosis of AGC patients. Quantitative comparison monitoring multiploid CTCs before and after therapy may help predict improved or inferior prognosis and chemoresistance. | Yilin Li Xiaotian Zhang Jifang Gong Qiyue Zhang Jing Gao Yanshuo Cao Daisy Dandan Wang Peter Ping Lin Lin Shen | 2016 | Chinese Journal of Cancer Research2016，28，6： | 7 |
| 11 | Clinicopathologic features and treatment efficacy of Chinese patients with BRAF-mutated metastatic colorectal cancer: a retrospective observational study显示文摘Background: The prognostic role of the V600 E mutation of v-raf murine sarcoma viral oncogene homolog B1(BRAF)in metastatic colorectal cancer(mCRC) is well established, but the therapeutic regimen targeting this disease is lacking. This study aimed to analyze the clinicopathologic features of and treatment efficacy of commonly used regimens on BRAF-mutated mCRCs.Methods: We collected and reviewed the medical records of mCRC patients treated at Peking University Cancer Hospital & Institute(Beijing, China) between July 2011 and July 2016. Kirsten rat sarcoma viral oncogene homolog(KRAS),neuroblastoma RAS viral oncogene homolog(NRAS), and BRAF mutational status was assayed using direct sequencing. The details of clinicopathologic characteristics of patients and their responses to FOLFOXIRI regimen or standard therapy were obtained by reviewing the medical records. The progression-free survival(PFS) and overall survival(OS)were assessed using Kaplan-Meier analysis and compared using the log-rank test.Results: Of 1694 patients studied, 75 had BRAF exon 15 mutations. Of these 75 patients, 71 had V600 E mutation, 1 had D594 G mutation, 2 had K601 E mutation, and 1 had a novel T599_V600 insAGA alteration. No patients had KRAS or NRAS mutations. Of 63 patients with BRAFV600 E-mutated mCRC and sufficient clinical data, 27(42.9%) had right-sided colon tumors, 19(30.2%) had left-sided colon tumors, and 17(26.9%) had rectal tumors; 26(41.3%) had peritoneal metastases, and 50(79.4%) had distant lymph node metastases.The patients with BRAF K601 E-and T599_V600 insAGA-mutated tumors had similar clinicopathologic features to those with BRAFV600 E-mutated tumors. Patients with the BRAFV600 E mutation benefited more from FOLFOXIRI regimen compared with patients who underwent standard therapy(overall response rate 83.3% vs. 14.0%; median PFS 6.4 months vs. 2.8 months, P = 0.220; median OS11.0 months vs. 6.9 months, P = 0.048).Conclusions: BRAFV600 E mutations were commonly identified in right-sided tumors and showed a high incidence of peritoneal and distant lymph nodes metastases.This subtype of mCRC was characterized by short OS and unique patterns of metastasis. Compared with standard treatment regimens, the FOLFOXIRI regimen had acceptable and manageable toxicities and favorable efficacy on patients with BRAF-mutated mCRC. | Xicheng Wang Qing Wei Jing Gao Jian Li Jie Li Jifang Gong Yanyan Li Lin Shen | 2017 | Chinese Journal of Cancer2017，36，11： | 6 |
| 12 | Identification of differentially expressed genes in rat silicosis model by suppression subtractive hybridization analysis显示文摘在肺的纤维变性的发展的批评分子的机制仍然保持未知,让有差的预后的病人诊断。孤立在肺的纤维变性明确地起来调整的基因,我们建立了一个老鼠矽肺模型在有水晶的硅石暂停的治疗以后的 360 d。胸的拍证明一些散布高密度的阴影出现在肺地里。典型显微镜的小谎 rosing silicotic 小瘤在肺,牙槽的上皮的房间和支气管的上皮的房间形成了,特别地在部分 fibrosing silicotic 小瘤附近;他们中的一些显示出建议了在矽肺和肺癌症之间的关联的不正常的增生。抑制减少性的杂交分析被执行在肺织物把基因表示与矽肺和正常的肺织物作比较。反向的抄写聚合酶链反应证明七个新奇 cDNA 序列的表情由在有矽肺的肺织物的减少性的杂交不同于的抑制识别了正常的肺织物。生物信息学分析证明 47 积极克隆代表了包含二通常认为的蛋白质的 35 基因,四预言了类似的蛋白质。分析也证明一些在矽肺屏蔽了基因,例如 prolyl 4-hydroxylases,肌动朊相关的 protein-2/3 建筑群和酸的哺乳动物的 chitinase,以前没被报导。这些基因可以为在肺的纤维变性的发展调查分子的机制提供新线索。 | Zhongyuan Jin Baoan Liu Deyun Feng Chen Chen Xiang Li Yongbin Hu Jinwu Peng Yu Liu Jing Du Chunyan Fu Jifang Wen | 2008 | Acta Biochimica et Biophysica Sinica2008，40，8： | 6 |
| 13 | Temporal and Spatial Variations of Abundance of Phycocyaninand Phycoerythrin-Rich Synechococcus in Pearl River Estuary and Adjacent Coastal Area显示文摘Three surveys were carried out in Pearl River Estuary and adjacent coastal area in May, August, and November, 2013, to investigate the temporal and spatial variations of abundance of phycoerythrin-rich Synechococcus(PE-rich SYN) and phycocyanin-rich Synechococcus(PC-rich SYN). The effects of environmental factors on the alternation of the different Synechococcus groups were also elucidated. PE-rich SYN was detected in three surveys, whereas PC-rich SYN was detected in May and August, but not in November. The highest abundances of PE-rich SYN and PC-rich SYN were recorded in August and May, with mean values of 74.17×103 and 189.92×103 cells m L-1, respectively. From May to November, the relative abundance of PE-rich SYN increased, whereas that of PC-rich SYN declined. PE-rich and PC-rich SYN presented similar horizontal distributions with high abundance in the southern estuary in May, and in the western estuary in August. The abundances of PE-rich and PC-rich SYN were high at 27–32℃and salinity of 10–20. PC-rich SYN was not detected at < 24℃, and PC:PE-rich SYN decreased in abundance with salinity increase. When less than 20 mg L-1, suspended particulate matter(SPM) was helpful for Synechococcus growth. PE-rich SYN decreased in abundance when the concentration of dissolved inorganic nitrogen increased in May and November, and the concentration of phosphate increased in November. However, PC-rich SYN abundance and nutrients showed no correlation. Principal component analysis and regression analysis indicated that PE-rich SYN significantly correlated with the principal components that were affected by environmental factors. | JIANG Tao CHAI Chao WANG Jifang ZHANG Ling CEN Jingyi LU Songhui | 2016 | Journal of Ocean University of China2016，15，5： | 6 |
| 14 | Dark matter direct search sensitivity of the PandaX-4T experiment显示文摘The Panda X-4T experiment, a 4-ton scale dark matter direct detection experiment, is being planned at the China Jinping Underground Laboratory. In this paper we present a simulation study of the expected background in this experiment. In a 2.8-ton fiducial mass and the signal region between 1-10 keV electron equivalent energy, the total electron recoil background is found to be 4.9 × 10^(-5) kg^(-1) d^(-1) keV^(-1). The nuclear recoil background in the same region is 2.8 × 10^(-7) kg^(-1) d^(-1) keV^(-1). With an exposure of 5.6 ton-years, the sensitivity of Panda X-4 T could reach a minimum spin-independent dark matter-nucleon cross section of 6 × 10^(-48) cm^2 at a dark matter mass of 40 Ge V/c^2. | HongGuang Zhang Abdusalam Abdukerim Wei Chen Xun Chen YunHua Chen XiangYi Cui BinBin Dong DeQing Fang ChangBo Fu Karl Giboni Franco Giuliani LinHui Gu XuYuan Guo ZhiFan Guo Ke Han ChangDa He ShengMing He Di Huang XingTao Huang Zhou Huang Peng Ji XiangDong Ji YongLin Ju ShaoLi Li Yao Li Heng Lin HuaXuan Liu JiangLai Liu YuGang Ma YaJun Mao KaiXiang Ni JinHua Ning XiangXiang Ren Fang Shi AnDi Tan AnQing Wang Cheng Wang HongWei Wang Meng Wang QiuHong Wang SiGuang Wang XiuLi Wang XuMing Wang Zhou Wang MengMeng Wu ShiYong Wu JingKai Xia MengJiao Xiao PengWei Xie BinBin Yan JiJun Yang Yong Yang ChunXu Yu JuMin Yuan JianFeng Yue Dan Zhang Tao Zhang Li Zhao QiBin Zheng JiFang Zhou Ning Zhou XiaoPeng Zhou | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，3： | 6 |
| 15 | TGF-β1 alters microRNA profile in human gastric cancer cells显示文摘Objective: MicroRNAs (miRNAs) are important regulators that play a key role in tumorigenesis and tumor progression. Transforming growth factor-β1 (TGF-β1) is involved in invasion and metastasis in many tumors. In this study, we investigated the microRNAs (miRNA) profiles altered by TGF-β1 in gastric cancer (GC) cells. Methods: We detected the expression profiles of miRNA by miRNA microarray and quantitative real-time polymerase chain reaction. Migration and invasion, wound-healing assay, prediction of miRNA targets, Western blot and qRT-PCR analysis were carried out to determine the role of one selected miRNA, namely miR-193b, in affecting the biological behaviors of GC BGC823 cells. Results: Among 847 human miRNAs in the microarray, three miRNAs (miR-27a, miR-29b-1 and miR-194) were up-regulated and three (miR-574-3p, miR-193b and miR-130b) were down-regulated in BGC823 cells treated with TGF-β1 compared with control. miR-193b suppressed the invasion and metastasis of GC cells in vivo and in vitro, and down-regulated urokinase-type plasminogen activator (uPA) protein in GC cells. Conclusions: TGF-β1 altered miRNA expression profile in BGC823 cells. Among the altered miRNAs, TGF-β1 induced the down-regulation of miR-193b, which inhibited cell invasion and metastasis in vivo and in vitro, and down-regulated uPA protein in GC cells. | Haiyan Zhou Kuansong Wang Zhongliang Hu Jifang Wen | 2013 | Chinese Journal of Cancer Research2013，25，1： | 5 |
| 16 | A naturally occurring variation in the BrMAM-3 gene is associated with aliphatic glucosinolate accumulation in Brassica rapa leaves显示文摘Glucosinolate profiles significantly vary among Brassica rapa genotypes.However,the molecular basis of these variations is largely unknown.In this study,we investigated a major quantitative trait locus(QTL)controlling aliphatic glucosinolate accumulation in B.rapa leaves.The QTL,which encompasses three tandem MAM genes and two MYB genes,was detected in two BC2DH populations.Among the five-candidate genes,only the expression level of BrMAM-3(Bra013007)was significantly correlated with the accumulation of aliphatic glucosinolates in B.rapa leaves.We identified a naturally occurring insertion within exon 1 of BrMAM-3,which is predicted to be a loss-of-function mutation,as confirmed by qRT-PCR.We determined that the loss of function was associated with the low glucosinolate content in B.rapa accessions.Furthermore,overexpressing the BrMAM-3 gene resulted in an increase in total aliphatic glucosinolates in Arabidopsis transgenic lines.Our study provides insights into the molecular mechanism underlying the accumulation of aliphatic glucosinolates in B.rapa leaves,thereby facilitating in the manipulation of total aliphatic glucosinolate content in Brassica crops. | Jifang Zhang Hui Wang Zhiyuan Liu Jianli Liang Jian Wu Feng Cheng Shiyong Mei Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 5 |
| 17 | Expression Patterns of Cytochrome P450 Aromatase Genes During Ovary Development and Their Responses to Temperature Stress in Female Yellow Catfish (Pelteobagrus fulvidraco)显示文摘Cytochrome P450 aromatase(P450arom)plays a pivotal role in ovary development.In this study,we used semi-quanti-tative reverse transcription PCR(RT-PCR)to analyze spatiotemporal expressions of two P450arom genes(CYP19A and CYP19B)and their responses to temperature stress in female yellow catfish(Pelteobagrus fulvidraco).Tissue distribution pattern of CYP19 showed that CYP19B was abundantly expressed in fish brain and ovary(brain > ovary),but weakly in intestines,whereas CYP19A was exclusively expressed in ovary.Semi-quantitative RT-PCR analyses showed high transcript abundance of both CYP19A and CYP19B in the ovarian reproductive cycle,corresponding with serum estradiol-17β(E2)levels.Increases in aromatases,serum E2 and testosterone(T)levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages Ⅱ and Ⅴ during the ovarian cycle,whereas associated decreases in stage Ⅲ suggested vitellogenesis inhibition by heat stress.Gene expression of CYP19 was closely related to levels of serum E2.Results demonstrated CYP19 played a crucial role in the reproductive cycle of female yellow catfish.Different temperature stress affected CYP19 gene expression in the fish ovarian re-productive cycle.Associated P450arom genes could be useful for studying physiological aspects of yellow catfish. | LIU Miao WEN Haishen HE Feng LI Jifang SHI Dan HU Jian ZHANG Yuanqing MA Ruiqin MU Weijie QI Baoxia | 2011 | Journal of Ocean University of China2011，10，4： | 5 |
| 18 | Selection of reference genes for quantitative gene expression studies in the house fly (Musca domestica L.) using reverse transcription quantitative real-time PCR显示文摘 | Ming Zhong XiangWang Jifang Wen Jifeng Cai Chang Wu Sanaa Mohamed Ay | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 4 |
| 19 | Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)显示文摘Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P<0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P<0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder. | HE Feng WEN Haishen YU Dahui LI Jifang SHI Bao CHEN Caifang ZHANG Jiaren JIN Guoxiong CHEN Xiaoyan Shi Dan Yang Yanping | 2010 | Journal of Ocean University of China2010，9，4： | 4 |
| 20 | Analysis of DNA Methylation Level by Methylation-Sensitive Amplification Polymorphism in Half Smooth Tongue Sole(Cynoglossus semilaevis)Subjected to Salinity Stress显示文摘Increasingly arisen environmental constraints may contribute to heritable phenotypic variation including methylation changes,which can help the animals with development,growth and survival.In this study,we assessed the DNA methylation levels in three tissues(gonad,kidney and gill) of half smooth tongue sole under the salinity stress.The methylation-sensitive amplification polymorphism(MSAP) technique was applied to illustrate the regulation of epigenetic mechanism in environmental stimuli.Fish were subjected to 15 salinity treatment for 7 and 60 days,respectively.A total of 11259 fragments were amplified with 8 pairs of selective primers.The levels of methylated DNA in different tissues of females and males without salinity stress were analyzed,which were 32.76% and 47.32% in gonad;38.13% and 37.69% in kidney;37.58% and 34.96% in gill,respectively.In addition,the significant difference was observed in gonad between females and males,indicating that discrepant regulation in gonadal development and differentiation may involve sex-related genes.Further analysis showed that total and hemi-methylation were significantly decreased under 15 salinity for 7 days,probably resulting in up-regulating salt-tolerance genes expression to adjust salt changing.With the adjustment for 60 days,total and hemi-methylation prominently went back to its normal levels to obtain equilibrium.Particularly,full methylation levels were steady along with salinity stress to maintain the stability of gene expression.Additionally,the data showed that gonads in females and gills in males were superior in adaptability.As a result,DNA methylation regulates tissue-specific epiloci,and may respond to salinity stress by regulating gene expression to maintain animal survival and activity. | LI Siping HE Feng WEN Haishen LI Jifang SI Yufeng LIU Mingyuan HE Huiwen HUANG Zhengju | 2017 | Journal of Ocean University of China2017，16，2： | 4 |
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