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| 1 | Research Progress of Medicinal Secondary Metabolites and Gene Cloning of Dendrobium officinale显示文摘Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient content and high cost,limiting the use of D. officinale.Studying the network structure and rate-limiting steps of secondary metabolites of medicinal components of D. officinale,analyzing the secondary metabolic synthesis process,mastering the production rules of its medicinal components and carrying out gene cloning or biosynthesis,etc.are of great significance for the rational development and utilization of D. officinale resources. This paper briefly reviews the progress of the research on the secondary metabolites of D. officinale,including the detection and identification of metabolites and the identification and cloning of key metabolic enzymes. | Ye LIANG Caihua ZOU Jindu LI Shilong LI Tianzi LI | 2019 | Medicinal Plant2019，10，3： | 8 |
| 2 | Somatostatin receptor subtype 2-mediated scintigraphy and localization using ^(99m)Tc-HYNIC-Tyr^3-octreotide in human hepatocellular carcinoma-bearing nude mice显示文摘AIM: To investigate the uptake of 99mTc-HYNIC-Tyr3-octreotide (99mTc-HYNIC-TOC) in human hepatocellular carcinoma (HCC), which can provide the localizable diagnosis in hepatic carcinoma.METHODS: The expression of somatostatin receptor 2(SSTR2) messenger RNA (mRNA) in human HCC cell line HepG2 was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). Uptake of 99mTc-HYNIC-TOC was evaluated in the human HCC implanted into BALB/c nude mice. ANMIS2000 nuclear medicine analysis system was used to calculate the ratio of 99mTC uptake between tumor tissue and vital organs.RESULTS: We demonstrated the expression of SSTR2mRNA in human HCC cell line HepG2 by RT-PCR. The size of the RT-PCR products was 364 bp detected by sequence analysis of the human SSTR2 mRNA. Scintigraphy proved that 99mTc-HYNIC-TOC was uptaken in the tumor tissue,liver and kidney of the tumor-bearing mice.CONCLUSION: Based on expression of the SSTR2 mRNA in human NCC, 99mTc-NYNIC-TOC can markedly bind with and be uptaken by human HCC tissues as compared with normal liver tissue. The significant retention of radionuclide in kidney and bladder is probably related to non-specific peptide uptake in the tubulus cells of kidney and possibly due to excretion by kidney. Our results show that localizable diagnosis and targeting radiotherapy with radionuclidelabeled somatostatin analog for HCC are of great value to be further studied. | YongLi Jian-MingSi JunZhang JinDu FanWang BingJia | 2005 | World Journal of Gastroenterology2005，11，25： | 2 |
| 3 | The hybridization between peptide nucleic acid containing azobenzene and DNA labeled nanoparticle on chip surfaces studied by atomic force microscopy显示文摘Peptide nucleic acid containing azobenzene (N-PNA) was covalently bound on chip surfaces. Complementary DNA probes labeled by gold nanoparticles hybridizated with PNA on chip surfaces. The hybridization was successfully detected with AFM by the microscopy mothed. The influences of the position and the cis-trans isomerization of azobenzene unit on hybridization were investigated with AFM. | LI JinDu CHEN Miao ZHANG HaoBo LIU Sheng LIU JianXi | 2008 | Chinese Science Bulletin2008，53，19： | 1 |
| 4 | Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences显示文摘Hybridization of peptide nucleic acids probe containing azobenzene (NH2-TNT4, N-PNAs) with DNA was performed by covalently immobilizing of NH2-TNT4 in sequence on the 3-mercaptopropionic acid self-assembled monolayer modified gold electrode with the helps of N-(3-dimethylaminopropy1)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and the hybrid was coded as N-PNAs/DNA. Using [Fe(CN)6]4-/3- (1:1) as the electrochemical indicator, the electrochemical properties of the N-PNAs self-assembled monolayer (N-PNAs-SAMs) and N-PNAs/DNA hybridization system under the conditions of before and after UV light irradiation were characterized with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectra (EIS). Results showed that the redox currents decreased with the increase of irradiation time, suggesting that the ability of the charge transfer on the electrode surface was weakened and the conformation of hybrid system had been changed, and the control of PNAs/DNA hybridization could be realized by UV light irradiation. | LIU Sheng CHEN Miao LI JinDu ZHANG HaoBo WANG JinQing YANG ShengRong | 2009 | Science China Chemistry2009，52，7： | 1 |
| 5 | Pizza sauce spread classifreation using colour vision and support vector meekain 显示文摘 | Cheng Jindu Da Wansun | 2006 | Food Engireering2006，6，2： | 1 |
| 6 | Shape extraction and classification of pizza base using computer vision 显示文摘 | Cheng Jindu Da Wensun | 2004 | Journal of Food Engineering2004，64，4： | 1 |
| 7 | Synthesis and photo-activity of peptide nucleic acids containing an azobenzene unit显示文摘We prepared the azobenzene PNA unit and synthesized various PNAs containing an azobenzene unit by a typical tBoc method. Both the azobenzene PNA unit and PNA containing an azobenzene unit (N-PNA) showed reversible photoisomerization with UV and visible light irradiation. The hybridization of N-PNA with complementary DNA resulted in a considerable decrease of the photoisomerization rate constant of the azobenzene. | YUE ShanShan LI JinDu ZHANG JunYan LU JinJun CHEN Miao | 2009 | Chinese Science Bulletin2009，54，24： | 0 |
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