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| 1 | 3D bioprinting of a biomimetic meniscal scaffold for application in tissue engineering显示文摘Appropriate biomimetic scaffolds created via 3D bioprinting are promising methods for treating damaged menisci.However,given the unique anatomical structure and complex stress environment of the meniscus,many studies have adopted various techniques to take full advantage of different materials,such as the printing combined with infusion,or electrospining,to chase the biomimetic meniscus,which makes the process complicated to some extent.Some researchers have tried to tackle the challenges only by 3D biopringting,while its alternative materials and models have been constrained.In this study,based on a multilayer biomimetic strategy,we optimized the preparation of meniscus-derived bioink,gelatin methacrylate(GelMA)/meniscal extracellular matrix(MECM),to take printability and cytocompatibility into account together.Subsequently,a customized 3D bioprinting system featuring a dual nozzle+multitemperature printing was used to integrate the advantages of polycaprolactone(PCL)and meniscal fibrocartilage chondrocytes(MFCs)-laden GelMA/MECM bioink to complete the biomimetic meniscal scaffold,which had the best biomimetic features in terms of morphology and components.Furthermore,cell viability,mechanics,biodegradation and tissue formation in vivo were performed to ensure that the scaffold had sufficient feasibility and functionality,thereby providing a reliable basis for its application in tissue engineering. | Zhou Jian Tian Zhuang Tian Qinyu Peng Liqing Li Kun Luo Xujiang Wang Diaodiao Yang Zhen Jiang Shuangpeng Sui Xiang Huang Jingxiang Liu Shuyun Hao Libo Tang Peifu Yao Qi Guo Quanyi | 2021 | Bioactive Materials2021，6，6： | 3 |
| 2 | 3D-printed cell-free PCL-MECM scaffold with biomimetic micro-structure and micro-environment to enhance in situ meniscus regeneration显示文摘Despite intensive effort was made to regenerate injured meniscus by cell-free strategies through recruiting endogenous stem/progenitor cells,meniscus regeneration remains a great challenge in clinic.In this study,we found decellularized meniscal extracellular matrix(MECM)preserved native meniscal collagen and glycosaminoglycans which could be a good endogenous regeneration guider for stem cells.Moreover,MECM significantly promoted meniscal fibrochondrocytes viability and proliferation,increased the expression of type II collagen and proteoglycans in vitro.Meanwhile,we designed 3D-printed polycaprolactone(PCL)scaffolds which mimic the circumferential and radial collagen orientation in native meniscus.Taken these two advantages together,a micro-structure and micro-environment dually biomimetic cell-free scaffold was manipulated.This cell-free PCL-MECM scaffold displayed superior biocompatibility and yielded favorable biomechanical capacities closely to native meniscus.Strikingly,neo-menisci were regenerated within PCL-MECM scaffolds which were transplanted into knee joints underwent medial meniscectomy in rabbits and sheep models.Histological staining confirmed neo-menisci showed meniscus-like heterogeneous staining.Mankin scores showed PCL-MECM scaffold could protect articular cartilage well,and knee X-ray examination revealed same results.Knee magnetic resonance imaging(MRI)scanning also showed some neo-menisci in PCL-MECM scaffold group.In conclusion,PCL-MECM scaffold appears to optimize meniscus regeneration.This could represent a promising approach worthy of further investigation in preclinical applications. | Weimin Guo Mingxue Chen Zhenyong Wang Yue Tian Jinxuan Zheng Shuang Gao Yangyang Li Yufeng Zheng Xu Li Jingxiang Huang Wei Niu Shuangpeng Jiang Chunxiang Hao Zhiguo Yuan Yu Zhang Mingjie Wang Zehao Wang Jiang Peng Aiyuan Wang Yu Wang Xiang Sui Wenjing Xu Libo Hao Xifu Zheng Shuyun Liu Quanyi Guo | 2021 | Bioactive Materials2021，6，10： | 1 |
| 3 | Diagenetic Sequence and Genetic Mechanism of Silurian Tight Sandstone Reservoirs in the Eastern Tarim Basin, Northwest China显示文摘The Silurian stratigraphic sequence has recently become one of the most important exploration targets in the Tarim Basin, with a considerable amount of profitable hydrocarbon pools discovered in the central Tarim Basin. Previous exploration activities indicate that the Silurian stratigraphic sequence in the eastern Tarim Basin has great hydrocarbon exploration potential. The Silurian reservoirs comprise a set of tight marine sandstones, whose diagenetic sequence and genetic mechanism are still poorly understood. The complex relationship of hydrocarbon generation, the timing of the peak expulsion of the source rocks and the evolution of the reservoirs remains unclear. An integrated description and analysis have been carried out on core samples from eleven wells selected from the eastern Tarim Basin. A range of petrographic and geochemical analyses were conducted. By using an integrated approach with thin-section petrography, scanning electron microscopy(SEM), cathodoluminescence(CL), carbon and oxygen isotope geochemistry, formation water analysis, X-ray diffractometry(XRD), electron probe microanalysis and fluid inclusion microthermometry, the genesis and occurrence of individual diagenetic events were documented to reconstruct the diagenetic sequence and diagenetic model for the Silurian sandstone. Additionally, the tight nature of the Silurian reservoirs can mainly be attributed to the compaction processes and cementation. In particular, the destructiveness of the compactional processes to the original porosity is far greater than that from the cementation. Furthermore, fluid inclusion analyses also indicate that the Silurian sandstone has experienced three phases of hydrocarbon charge. The first two phases occurred during the eodiagenesis stage(from the Late Silurian to the Early Devonian and from the Late Carboniferous to the end of the Late Permian), when the Silurian sandstone was not tight and had a porosity of greater than 20%. The third phase occurred during the stage B of mesodiagenesis(since the Late Cretaceous), when the Silurian sandstone was fully tight. | Jingxiang Guo Qiang Li Wenwen Wang Qian Zhang Junhui Wang Zhiteng Hou | 2017 | Journal of Earth Science2017，28，6： | 1 |
| 4 | A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells显示文摘 | Qiang Yang Jiang Peng Quanyi Guo Jingxiang Huang Li Zhang Jun Yao Fei Yang Shenguo Wang Wenjing Xu Aiyuan Wang Shibi Lu | 2008 | Biomaterials2008，，： | 1 |
| 5 | Reservoir Characteristics and Controlling Factors of Silurian Lower Kepingtage Formation in Tahe Area, Tarim Basin, NW China显示文摘With the breakthrough of exploration in Well TP16-1, the lower Kepingtage Formation becomes a key target for petroleum exploration of deep clastic reservoir in Tahe area. In this paper we focused on the research of the reservoir characteristics and its controlling factors in two sub-member formations(S_1k_1~1 and S_1k_1~3). Based on X-ray diffraction, conventional physical properties data(porosity and permeability) and reservoir storage space data(casting thin section and scanning electron microscope), we determined that the S_1k_1 Formation belongs to extra-low porosity and permeability reservoir, although the upper S_1k_1~3 Formation shows relative better physical characteristic than the lower S_1k_1~1 Formation. The development of storage space in the study area is controlled by sedimentary microfacies, diagenesis process. Reservoirs in S_1k_1 Formation are mainly located in channel(S_1k_1~1 sandstones) and sand flat(S_1k_1~3 sandstones). The sand flat sediments with a more coarse grain size compared with the channel. In diagenesis, compaction is the major controlling factor for reducing the porosity, followed by cementation. Dissolution of diagenesis is the major controlling factor in enhancing the reservoir porosities. Compared with channel(S_1k_1~1) sandstones, sand flat sandstones(S_1k_1~3) have better reservoir quality for its weaker compaction, cementation and stronger dissolution. On the basis of sedimentary characteristics(grain size and subfacies), physical property(porosity and permeability) and reservoir storage space, we divide the S_1k_1 reservoir into three categories(I, II and III). Type I reservoir is high quality reservoir. It is mainly distributed in the south area of S_1k_1~1 and S_1k_1~3 reservoir. Type II is moderate reservoir. It is located in the middle of S_1k_1~1 reservoir and in the north of S_1k_1~3 reservoir. Type III is the poor reservoir. It is only located in the north of S_1k_1~1 reservoir. | Ruohan Liu Zaixing Jiang Ming Wang Weili Yang Jingxiang Guo Minghao Wu Yi Gao Shanyazi Wei Zhihong Nie Hong He | 2017 | Journal of Earth Science2017，28，6： | 0 |
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