| 1 | Genomic and Phenotypic Diversity of Carbapenemase-Producing Enterobacteriaceae Isolates from Bacteremia in China: A Multicenter Epidemiological, Microbiological, and Genetic Study显示文摘Carbapenemase-producing Enterobacteriaceae(CPE) isolates are recognized as one of the most severe threats to public health. However, the population structure and genetic characteristics of CPE isolates among bloodstream infections(BSIs) are largely unknown. To address this knowledge gap, in this study,we included patients with clinically significant BSIs due to Enterobacterales isolates, recruited from 26 sentinel hospitals in China(2014–2015). CPE isolates were microbiologically and genomically characterized,including their susceptibility profiles, molecular typing, phylogenetic features, and genetic context analysis of carbapenemase-encoding genes. Of the 2569 BSI Enterobacterales isolates enrolled, 42(1.6%) were carbapenemase-positive. Moreover, among the 2242 investigated isolates, 1111(49.6%) extendedspectrum β-lactamase(ESBL)-producing isolates were identified in Escherichia coli(E. coli), Klebsiella pneumoniae(K. pneumoniae), Proteus mirabilis(P. mirabilis), and Klebsiella oxytoca. Whole genome sequencing analysis showed the clonal spread of K. pneumoniae carbapenemase(KPC)-2-producing K. pneumoniae sequence type(ST) 11 and New Delhi metallo-β-lactamase(NDM)-5-producing E. coli ST167 in our collection. Plasmid analysis revealed that carbapenemase-encoding genes were located on multiple plasmids. A high prevalence of biofilm-encoding type 3 fimbriae clusters and yesiniabactin-associated genes was observed in K. pneumoniae isolates. This work demonstrates the high prevalence of ESBLs and the wide dissemination of CPE among BSI isolates in China, which represent real clinical threats. Moreover, our findings first illustrate a more comprehensive genome scenario of CPE isolates among BSIs. The clonal spread of KPC-2-producing K. pneumoniae ST11 and NDM-5-producing E. coli ST167 needs to be closely monitored. | Beiwen Zheng Hao Xu Lihua Guo Xiao Yu Jinru Ji Chaoqun Ying Yunbo Chen Ping Shen Huiming Han Chen Huang Shuntian Zhang Tao Lv Yonghong Xiao | 2022 | Engineering2022，8，5： | 2 |
| 2 | M1 macrophage-related gene model for NSCLC immunotherapy response prediction显示文摘Patients diagnosed with non-small cell lung cancer(NSCLC)have a limited lifespan and exhibit poor immunotherapy outcomes.M1 macrophages have been found to be essential for antitumor immunity.This study aims to develop an immunotherapy response evaluation model for NSCLC patients based on transcription.RNA sequencing profiles of 254 advanced-stage NSCLC patients treated with immunotherapy are downloaded from the POPLAR and OAK projects.Immune cell infiltration in NSCLC patients is examined,and thereafter,different coexpressed genes are identified.Next,the impact of M1 macrophage-related genes on the prognosis of NSCLC patients is investigated.Six M1 macrophage coexpressed genes,namely,NKX2-1,CD8A,SFTA3,IL2RB,IDO1,and CXCL9,exhibit a strong association with the prognosis of NSCLC and serve as effective predictors for immunotherapy response.A response model is constructed using a Cox regression model and Lasso Cox regression analysis.The M1 genes are validated in our TD-FOREKNOW NSCLC clinical trial by RT-qPCR.The response model shows excellent immunotherapy response prediction and prognosis evaluation value in advanced-stage NSCLC.This model can effectively predict advanced NSCLC prognosis and aid in identifying patients who could benefit from customized immunotherapy as well as sensitive drugs. | Sifan Wu Qiqi Sheng Pengjun Liu Zhe Jiao Jinru Lv Rong Qiao Dongkun Xie Zanhan Wang Jiamei Ge Penghui Li Tiaoxia Wei Jie Lei Jieyi Fan Liang Wang | 2024 | Acta Biochimica et Biophysica Sinica2024，56，3： | 0 |
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