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| 1 | miRNA-dysregulation associated with tenderness variation induced by acute stress in Angus cattle显示文摘miRNAs are a class of small, single-stranded, non-coding RNAs that perform post-transcriptional repression of target genes by binding to 3' untranslated regions. Research has found that miRNAs involved in the regulation of many metabolic processes. Here we uncovered that the beef quality of Angus cattle sharply diversified after acute stress. By performing miRNA microarray analysis, 13 miRNAs were significantly differentially expressed in stressed group compared to control group. Using a bioinformatics method, 135 protein-coding genes were predicted as the targets of significant differentially expressed miRNAs. Gene Ontology (GO) term and Ingenuity Pathway Analysis (IPA) mined that these target genes involved in some important pathways, which may have impact on meat quality and beef tenderness. | Chunping Zhao Fei Tian Ying Yu George Liu Linsen Zan M Scott Updike Jiuzhou Song | 2012 | Journal of Animal Science and Biotechnology2012，3，2： | 2 |
| 2 | Efficient grain refinement of Al alloys induced by in-situ nanoparticles显示文摘Grain refinement of Al and Al alloys during the solidification remains a long-term challenge.Here we report a general strategy for efficient grain refinement through instantaneously in-situ forming abundant nanoparticles with concentrated size distribution.The Al-Zn-Mg-Cu alloy and commercial purity Al are respectively refined by using this proposed strategy and the traditional refinement method(adding AlTi-B alloy into Al melt),and the experimental and numerical results indicate that the TiBnanoparticles instantaneously form in the Al melt during the in-situ refinement process.Compared to the traditional refinement treatment method,the new approach not only can promote the high number density of the nucleation sites and narrow their size distribution,but also tremendously attenuates the agglomeration and settlement of the heterogeneous nucleation sites.It exhibits much better grain refinement ability and inhibits the decline of the grain refinement efficiency.This approach may have broad application prospect not only for the grain refinement of Al and Al alloys,but also for the grain refinement of other alloy systems,e.g.steel,magnesium,copper,and so on. | Hongxiang Jiang Yan Song Lili Zhang Jie He Shixin Li Jiuzhou Zhao | 2022 | Journal of Materials Science & Technology2022，，29： | 1 |
| 3 | UBE2I stimulates female gonadal differentiation in chicken(Gallus gallus)embryos显示文摘Without known analogous sex-determining factors like SRY(sex determining region Y)in mammals,the chicken(Gallus gallus)sex determination mechanism still remains unclear,which highly restricts the biological research on chicken development and poultry single-sex reproduction.Here we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR,but also described the functional role of UBE2I in the gonadal development of chicken embryos.Results showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs(primordial germ cells)in embryonic gonads and robust expression in ovaries of newborn chickens.Most importantly,we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular injection.The qRT-PCR analysis showed that the sex-related genes(FOXL2,CYP19A1 and HINTW)in females were upregulated(P<0.05)under the overexpression of UBE2I and the sex-related genes(SOX9,DMRT1 and WT1)in females were downregulated(P<0.05)after interfering UBE2I.Furthermore,the change of UBE2I expression was associated with the level of estradiol and its receptors(AR and ESR),which suggests that UBE2I is necessary to initiate the female-specific development in chickens.In conclusion,this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens,which provides insights for further understanding the mechanism of sex determination in chickens. | JIN Kai ZHOU Jing ZUO Qi-sheng LI Jian-cheng Jiuzhou SONG ZHANG Ya-ni CHANG Guo-bing CHEN Guo-hong LI Bi-chun | 2021 | Journal of Integrative Agriculture2021，20，11： | 0 |
| 4 | Genetic assessment of inbred chicken lines indicates genomic signatures of resistance to Marek's disease显示文摘Background: Marek's disease(MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. However, the mechanisms of genetic resistance for MD are complex and not fully understood. MD-resistant line 6_3 and MD-susceptible line 7_2 are two highly inbred progenitor lines of White Leghorn. Recombinant Congenic Strains(RCS) were developed from these two lines, which show varied susceptibility to MD.Results: We investigated genetic structure and genomic signatures across the genome, including the line 6_3 and line7_2, six RCSs, and two reciprocally crossed flocks between the lines 6_3 and 7_2(F1 6_3× 7_2 and F1 7_2× 6_3) using Affymetrix~? Axiom~? HD 600 K genotyping array. We observed 18 chickens from RCS lines were specifically clustered into resistance sub-groups distributed around line 6_3. Additionally, homozygosity analysis was employed to explore potential genetic components related to MD resistance, while runs of homozygosity(ROH) are regions of the genome where the identical haplotypes are inherited from each parent. We found several genes including SIK, SOX1, LIG4, SIK1 and TNFSF13B were contained in ROH region identified in resistant group(line 6_3 and RCS), and these genes have been reported that are contribute to immunology and survival. Based on F_(ST) based population differential analysis, we also identified important genes related to cell death and anti-apoptosis, including AKT1, API5, CDH13, CFDP and USP15,which could be involved in divergent selection during inbreeding process.Conclusions: Our findings offer valuable insights for understanding the genetic mechanism of resistance to MD and the identified genes could be considered as candidate biomarkers in further evaluation. | Lingyang Xu Yanghua He Yi Ding George E.Liu Huanmin Zhang Hans H.Cheng Robert L.Taylor Jr. Jiuzhou Song | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 0 |
| 5 | Diet induced the change of mtDNA copy number and metabolism in Angus cattle显示文摘Background: Grass-fed and grain-fed Angus cattle differ in the diet regimes. However, the intricate mechanisms of different beef quality and other phenotypes induced by diet differences are still unclear. Diet affects mitochondrial function and dynamic behavior in response to changes in energy demand and supply. In this study, we examined the mtDNA copy number, mitochondria-related genes expression, and metabolic biomarkers in grass-fed and grainfed Angus cattle.Results: We found that the grass-fed group had a higher mtDNA copy number than the grain-fed group. Among different tissues, the mtDNA copy number was the highest in the liver than muscle, rumen, and spleen. Based on the transcriptome of the four tissues, a lower expression of mtDNA-encoded genes in the grass-fed group compared to the grain-fed group was discovered. For the mitochondria-related nuclear genes, however, most of them were significantly down-regulated in the muscle of the grass-fed group and up-regulated in the other three tissues. In which, COX6 A2, POLG2, PPIF, DCN, and NDUFA12, involving in ATP synthesis, mitochondrial replication,transcription, and maintenance, might contribute to the alterations of mtDNA copy number and gene expression.Meanwhile, 40 and 23 metabolic biomarkers were identified in the blood and muscle of the grain-fed group compared to a grass-fed group, respectively. Integrated analysis of the altered metabolites and gene expression revealed the high expression level of MDH1 in the grain-fed group might contribute to the mitochondrial NADH oxidation and spermidine metabolism for adapting the deletion mtDNA copy number.Conclusions: Overall, the study may provide further deep insight into the adaptive and regulatory modulations of the mitochondrial function in response to different feeding systems in Angus cattle. | Ying Bai José A.Carrillo Yaokun Li Yanghua He Jiuzhou Song | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 0 |
| 6 | gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭显示文摘以马立克病病毒(MDV)感染SPF白来航鸡,收集脾和肝来源的淋巴瘤,利用实时荧光定量PCR(qRTPCR)进行检测,结果显示gga-miR-140-3p在感染MDV的肿瘤化脾和肝淋巴瘤中低表达。为揭示该miRNA在马立克病(MD)肿瘤转化过程中的作用,以该miRNA为研究对象,MDV转化细胞系MDCC-MSB1为试验材料,分别转染miRNA激动剂或阴性对照,检测细胞增殖和迁移,并检测与细胞侵袭密切相关的基因MMP2和MMP9mRNA表达情况。结果显示,与阴性对照组相比,转染gga-miR-140-3p激动剂后,细胞增殖在24、36和48h后发生显著(P<0.05)抑制,在60和72h后发生极显著(P<0.01)抑制。转染激动剂后,细胞迁移数也显著(P<0.05)减少。转染激动剂后,MMP2在转染后24h转录显著下调(P<0.05),转染后48和72h转录极显著(P<0.01)下调,MMP9基因在转染后48h转录显著(P<0.05)下调。gga-miR-140-3p作为在MDV感染组织中异常表达的miRNA,能够影响MD肿瘤淋巴细胞的特征——增殖、迁移和侵袭,提示该miRNA可能参与MD肿瘤转化过程。 | 赵春芳 李新 韩博 曲鲁江 刘长军 Jiuzhou Song 连玲 杨宁 | 2018 | 畜牧兽医学报2018，49，5： | 0 |
| 7 | A Model Describing Solidification Microstructure Evolution in an Inoculated Aluminum Alloys显示文摘A population dynamics-cellular automaton(PD-CA)model is developed to describe the microstructure formation in an inoculated Al alloys.The model involves the dynamics behaviors of the inoculated particles and the nucleation,initial spherical growth of nuclei as well as the subsequent dendritic growth.The model was validated by using the experimental results for the Al-Cu alloys inoculated by Al–Ti–C refiner first,and then used to simulate the detailed solidification process in an inoculated Al–Cu alloy.The results indicate that the TiC is not stable in Al melt.The heterogeneous nucleation process consists of two stages:a very short initial stage dominated by the cooling rate and the later stage dominated by the number of the active TiC.It stops at the very moment the recalescence occurs.The average grains size d of the aluminum alloys aw))inoculated by the Al–Ti–C refiner can be calculated by d(μm)=a·exp(t/60·In(C_(0)/w)/(v_cool·^(t)^(1/3)w(1/3)+b·Int/Q·v^(1/2))cool where Q is the cool factor,C0(%)is the initial solutes composition,w(%)is the additive amount of Al–Ti–C refiner.t(min)is the holding temperature time since the Al–Ti–C refiner is added into the melt.a and b are the constants. | Yan Song Hongxiang Jiang Lili Zhang Shixin Li Jiuzhou Zhao Jie He | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，6： | 0 |
| 8 | The eQTL colocalization and transcriptome‑wide association study identify potentially causal genes responsible for economic traits in Simmental beef cattle显示文摘Background A detailed understanding of genetic variants that affect beef merit helps maximize the efficiency of breeding for improved production merit in beef cattle.To prioritize the putative variants and genes,we ran a com-prehensive genome-wide association studies(GWAS)analysis for 21 agronomic traits using imputed whole-genome variants in Simmental beef cattle.Then,we applied expression quantitative trait loci(eQTL)mapping between the genotype variants and transcriptome of three tissues(longissimus dorsi muscle,backfat,and liver)in 120 cattle.Results We identified 1,580 association signals for 21 beef agronomic traits using GWAS.We then illuminated 854,498 cis-eQTLs for 6,017 genes and 46,970 trans-eQTLs for 1,903 genes in three tissues and built a synergistic network by integrating transcriptomics with agronomic traits.These cis-eQTLs were preferentially close to the transcription start site and enriched in functional regulatory regions.We observed an average of 43.5%improvement in cis-eQTL discovery using multi-tissue eQTL mapping.Fine-mapping analysis revealed that 111,192,and 194 variants were most likely to be causative to regulate gene expression in backfat,liver,and muscle,respectively.The transcriptome-wide association studies identified 722 genes significantly associated with 11 agronomic traits.Via the colocalization and Mendelian randomization analyses,we found that eQTLs of several genes were associated with the GWAS signals of agronomic traits in three tissues,which included genes,such as NADSYN1,NDUFS3,LTF and KIFC2 in liver,GRAMD1C,TMTC2 and ZNF613 in backfat,as well as TIGAR,NDUFS3 and L3HYPDH in muscle that could serve as the candidate genes for economic traits.Conclusions The extensive atlas of GWAS,eQTL,fine-mapping,and transcriptome-wide association studies aid in the suggestion of potentially functional variants and genes in cattle agronomic traits and will be an invaluable source for genomics and breeding in beef cattle. | Wentao Cai Yapeng Zhang Tianpeng Chang Zezhao Wang Bo Zhu Yan Chen Xue Gao Lingyang Xu Lupei Zhang Huijiang Gao Jiuzhou Song Junya Li | 2023 | Journal of Animal Science and Biotechnology2023，14，5： | 0 |
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