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8篇 您的检索式:作者名="Johannah"
    题名 作者 年代 出处 被引量
1A simple comet assay for archived sperm correlates DNA fragmentation to reduced hyperactivation and penetration of zonafree hamster oocytes显示文摘Philip JC Johannah UC William CP 2001Fertil Steril2001,75,:1
2Temperature variable and the efficiency of sperm mediated transfection of HPV16 DNA into cells显示文摘To pretreat sperm at various temperatures before exposure to human papillomavirus(HPV)16DNA fragments and to assess the efficiency of HPV carrier sperm to transfect cumulus cells.Methods:Cumulus cells from follicular aspirates were obtained,pooled and divided into culture dishes containing Syby Gold-stainedHPVDNA carrying spern that were either pretreated at4℃,37℃or40℃(n=5).The cells were incubated in5%Co2in air mixture at 37℃for 24hours.The effeiciency of sperm to take up fluorescent HPVDNAwas determined at hour0.After incubation.cumulus cell viability was assessed using the eosin method and the percentages of fluorescent cumulus cells determined.Results:Ouver half of all the cumulus cells became fluorescent with the highest percentage in the 37℃group.Sperm pretreated at 4℃had the greatest amount of HPVDNAfragmen ts.Total spern motility was similar for the3pretreatment groups.There were no differences in cumulus viability among the groups.Conclusion:Sperm pretreated at37℃transferred the greatest amount of fluorescent HPVDNAfragments to the cumulus cells.The HPVDNAwas observed in the nuclear and cytoplasmic compartments.The data suggested the possibility of sperm as a vector for the transmission of HPVDNAto the cumulus cells surrounding ovulated oocytes,which might lead to early implantation failures.Ruslana Kadze Philip J. Chan John D. Jacobson Johannah U. Corselli Alan King 2002Asian Journal of Andrology2002,4,3:1
3Common genetic variation in eight genes of the GH/IGF1 axis does not contribute to adult height variation显示文摘Guillaume Lettre Johannah L. Butler Kristin G. Ardlie Joel N. Hirschhorn 2007Human Genetics2007,,2:1
4A growth maximizing tax structure for New Zealand 显示文摘JOHANNAH BRANSON C A KNOX LOVELL 2001International Tax and Public Fi- nance2001,18,2:1
5Isolation of plant DNA from fresh tissue显示文摘DOYLE J J JOHANNAH L D 1990Focus1990,12,1:1
6Muscle energy technique for chronic obstructive pulmonary disease:A feasibility study显示文摘Objective:This study aimed to investigate the feasibility of implementing a manual therapy technique(muscle energy technique,MET)protocol in a hospital pulmonary rehabilitation(PR)program for patients with moderate to severe chronic obstructive pulmonary disease(COPD).Methods:Participants aged 40 years and over,with moderate to severe COPD,were recruited into this 12-week study.The primary outcome measures were feasibility(acceptability of the intervention and attendance/adherence to the trial)and safety(adverse events,AEs).All participants received the MET and PR therapies.Participants and assessors were unblinded.Semi-standardized MET was delivered on 6 occasions(a maximum of once per week)at the hospital directly before a PR session.Participants undertook PR sessions as per the hospital program at a frequency of two days per week for 8 weeks.Participants were contacted 4 weeks after their final MET treatment via a telephone call to assess acceptability of the intervention.Results:Thirty-three participants were enrolled,with a median age of 74 years(range 45-89 years).The median number of MET sessions that participants attended was 5(range O-6)out of a possible 6 sessions(83%attendance).At follow-up,participants overwhelmingly enjoyed the MET treatment with some subjectively reporting improved breathing.There were no major AEs related to the intervention,with the majority of AEs classified as expected events related to COPD exacerbations.Conclusion:It is feasible to implement a manual therapy protocol using MET as an adjunct to PR in a hospital setting.Recruitment rates were satisfactory and there were no AEs related to the MET component of the intervention.Danielle A.Baxter Meaghan E.Coyle Catherine J Hill Christopher Worsnop Johannah L.Shergis 2023Journal of Integrative Medicine2023,21,3:0
7替代齿式简便和节约的至上选择显示文摘本文中我们将会探讨具体案例,并讨论铁姆肯公司Timken Quick-Flex 弹性联轴器如何在不利环境下成为替代选择,以及如何弥补普通齿轮式联接的不足。Matt Bamford Tyler Coffey Johannah Nelson 2016现代制造2016,0,14:0
8A picture scroll that never stops unfolding显示文摘The other day, I visited a museum in Shaoxing’s Dongpu town that displayed a scroll running around all four walls of the room. It represents the people of Shaoxing going about their daily lives. The guide said the15-meter-long work depicts the most human figures in a painting anywhere in the world and is considered to be the Shaoxing equivalent of the Qingming Scroll from the Song Dynasty (960-1279).Johannah Cook 2019Voice of Friendship2019,,1:0
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