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您的检索式：作者名="Joseph Horwitz"
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| 1 | PPA与出血性中风相关性显示文摘背景:PPA(phenylpropanolamine,苯丙醇胺)常见于食欲抑制药和咳嗽感冒药中,以往病例报告表明含PPA的药物与出血性中风有关,通常发生在首次使用后。为阐明二者相关性,我们设计了一项病例对照研究。方法:18~49岁的男、女性研究病例来自美国43所医院,其合格标准包括在参与项目前30天内发生蛛网膜下腔出血或颅内出血,而既往并无确诊的脑损伤史。采用随机电话拨号,为每个病例安排2例对照进行面访。结果:调查人群包括702名病例和1376名对照。女性服用含PPA食欲抑制药和出血性中风危险之间相关联的调整比值比为16.58(95%可信区间1.51~182.21,P=0.02);女性首次服用含PPA药物与出血性中风危险之间关联的调整比值比3.13(95%可信区间0.86~11.46,P=0.08),所有首次服用的PPA皆为咳嗽感冒药。男、女性调查人群服用含PPA药物与出血性中风危险二者关联的调整比值比为1.49(95%可信区间0.84~2.64.P=0.17);男、女性调查人群服用含PPA的咳嗽感冒药与出血性中风危险二者关联的调整比值比为1.23(95%可信区间0.68~2.24,P=0.49),服用舍PPA的食欲抑制药与出血性中风危险关联的调整比值比为15.92(95%可信区间1.38~184.13,p=0.03)。对男性的分析结果表明:服用含PPA的咳嗽感冒药未引起出血性中风危险增加,男性无一例使用? | Walter N. Kernan Catherine M. Viscoli Lawrence M. Brass Joseph P. Broderick Thomas Brott Edward Feldmann Lewis B. Morgenstern Janet Lee Wilterdink Ralph I. Horwitz 陈关君 | 2001 | 药物不良反应杂志2001，3，1： | 1 |
| 2 | Major Risk Factors for Intracerebral Hemorrhage in the Young Are Modifiable显示文摘 | Edward Feldmann Joseph P. Broderick Walter N. Kernan Catherine M. Viscoli Lawrence M. Brass Thomas Brott Lewis B. Morgenstern Janet Lee Wilterdink Ralph I. Horwitz | 2005 | Stroke2005，，9： | 1 |
| 3 | Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production显示文摘The generation of stable clones for biomolecule production is a common but lengthy and labor-intensive process.For complex molecules,such as viruses or virus-like particles(VLPs),the timeline becomes even more cumbersome.Thus,in the early stages of development,transient production methods serve as a reasonable alternative to stable clone construction.In this work,an investigation of a polyethylenimine-(PEI-)based transfection method for the transient production of Chikungunya(Chik)VLPs,a vaccine candidate molecule,was undertaken.This effort focuses on tracking cell population responses during transfection,understanding how process changes affect these responses,and monitoring patterns in cell performance over the culture duration.Plasmid labeling and VLP staining were employed to comprehensively track cells via flow cytometry and to draw correlations between plasmid DNA(pDNA)uptake and the resulting VLP expression.The method detected high transfection efficiency(≥97%)in all samples tested and demonstrated the capability to track kinetics of plasmid-cell binding.With varied transfection cell concentrations,the pDNA binding kinetics are altered and saturation binding is observed in the lowest cell concentration sample tested in less than 3 hours of incubation.Interestingly,in all samples,the flow cytometry analysis of relative pDNA amount versus VLP expression staining showed that cells which contained fewer pDNA complexes resulted in the highest levels of VLP stain.Finally,to determine the potential breadth of our observations,we compared daily expression patterns of ChikVLP with a reporter,monomeric GFP molecule.The similarities detected suggest the interpretations presented here to likely be more broadly informative and applicable to PEI-based transient production of additional biological products as well. | Daniel J.Blackstock Alvenne Goh Shamitha Shetty Giulia Fabozzi Rong Yang Vera BIvleva Richard Schwartz Joseph Horwitz | 2020 | Research2020，，1： | 0 |
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